scholarly journals Real-Time Nanomicroscopy via Three-Dimensional Single-Particle Tracking

ChemPhysChem ◽  
2009 ◽  
Vol 10 (14) ◽  
pp. 2458-2464 ◽  
Author(s):  
Yoshihiko Katayama ◽  
Ondrej Burkacky ◽  
Martin Meyer ◽  
Christoph Bräuchle ◽  
Enrico Gratton ◽  
...  
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Three Dimensional ◽  
Single Particle Tracking

Adaptive optics enables three-dimensional single particle tracking at the sub-millisecond scale

2013 ◽  
Vol 102 (17) ◽  
pp. 173702 ◽  
Author(s):  
Manuel F. Juette ◽  
Felix E. Rivera-Molina ◽  
Derek K. Toomre ◽  
Joerg Bewersdorf
Keyword(s):  
Adaptive Optics ◽  
Particle Tracking ◽  
Single Particle ◽  
Three Dimensional ◽  
Single Particle Tracking

Three-Dimensional Single Particle Tracking and Its Applications in Confined Environments

2020 ◽  
Vol 13 (1) ◽  
pp. 381-403
Author(s):  
Yaning Zhong ◽  
Gufeng Wang
Keyword(s):  
Mass Transport ◽  
Particle Tracking ◽  
Single Particle ◽  
Three Dimensional ◽  
Surface Interactions ◽  
Single Particle Tracking ◽  
Confined Space ◽  
Design And Optimization ◽  
Chemical Recognition ◽  
Molecule Surface

Single particle tracking (SPT) has proven to be a powerful technique in studying molecular dynamics in complicated systems. We review its recent development, including three-dimensional (3D) SPT and its applications in probing nanostructures and molecule-surface interactions that are important to analytical chemical processes. Several frequently used 3D SPT techniques are introduced. Especially of interest are those based on point spread function engineering, which are simple in instrumentation and can be easily adapted and used in analytical labs. Corresponding data analysis methods are briefly discussed. We present several important case studies, with a focus on probing mass transport and molecule-surface interactions in confined environments. The presented studies demonstrate the great potential of 3D SPT for understanding fundamental phenomena in confined space, which will enable us to predict basic principles involved in chemical recognition, separation, and analysis, and to optimize mass transport and responses by structural design and optimization.

scholarly journals Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2826 ◽  
Author(s):  
Shangguo Hou ◽  
Courtney Johnson ◽  
Kevin Welsher
Keyword(s):  
Fluorescence Spectroscopy ◽  
Real Time ◽  
Temporal Resolution ◽  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fluorescence Spectroscopy ◽  
Active Feedback

Single molecule fluorescence spectroscopy has been largely implemented using methods which require tethering of molecules to a substrate in order to make high temporal resolution measurements. However, the act of tethering a molecule requires that the molecule be removed from its environment. This is especially perturbative when measuring biomolecules such as enzymes, which may rely on the non-equilibrium and crowded cellular environment for normal function. A method which may be able to un-tether single molecule fluorescence spectroscopy is real-time 3D single particle tracking (RT-3D-SPT). RT-3D-SPT uses active feedback to effectively lock-on to freely diffusing particles so they can be measured continuously with up to photon-limited temporal resolution over large axial ranges. This review gives an overview of the various active feedback 3D single particle tracking methods, highlighting specialized detection and excitation schemes which enable high-speed real-time tracking. Furthermore, the combination of these active feedback methods with simultaneous live-cell imaging is discussed. Finally, the successes in real-time 3D single molecule tracking (RT-3D-SMT) thus far and the roadmap going forward for this promising family of techniques are discussed.

Robust real-time 3D single-particle tracking using a dynamically moving laser spot

2017 ◽  
Vol 42 (12) ◽  
pp. 2390 ◽  
Author(s):  
Shangguo Hou ◽  
Xiaoqi Lang ◽  
Kevin Welsher
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Laser Spot

Photoinduced mass transfer of azo polymers from micrometer to submillimeter studied by a real-time single particle strategy

Soft Matter ◽  
2020 ◽  
Vol 16 (42) ◽  
pp. 9746-9757
Author(s):  
Hao Huang ◽  
Chen Zhang ◽  
Jiaxing Lan ◽  
Zenan Wang ◽  
Xiaogong Wang
Keyword(s):  
Mass Transfer ◽  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Azo Polymers ◽  
Tracking Strategy

This article reports a real-time single particle tracking strategy to investigate the photoinduced mass transfer of azo polymers and the results.

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