scholarly journals Photophysical Aspects of Single-Molecule Detection by Two-Photon Excitation with Consideration of Sequential Pulsed Illumination

ChemPhysChem ◽  
2004 ◽  
Vol 5 (7) ◽  
pp. 921-921 ◽  
Author(s):  
R. Niesner ◽  
W. Roth ◽  
Karl-Heinz Gericke
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Single-molecule detection by two-photon excitation of fluorescence

1997 ◽  
Author(s):  
Christoph Zander ◽  
Leif Brand ◽  
C. Eggeling ◽  
Karl-Heinz Drexhage ◽  
Claus A. M. Seidel
Keyword(s):  
Single Molecule ◽  
Single Molecule Detection ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

3D single-molecule tracking using one- and two-photon excitation microscopy

2014 ◽  
Author(s):  
Cong Liu ◽  
Evan P. Perillo ◽  
Quincy Zhuang ◽  
Khang T. Huynh ◽  
Andrew K. Dunn ◽  
...  
Keyword(s):  
Single Molecule ◽  
Single Molecule Tracking ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity

2018 ◽  
Vol 20 (45) ◽  
pp. 28487-28498 ◽  
Author(s):  
Rachel S. Fisher ◽  
David Nobis ◽  
Anders F. Füchtbauer ◽  
Mattias Bood ◽  
Morten Grøtli ◽  
...  
Keyword(s):  
Single Molecule ◽  
Ultrasensitive Detection ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Ultrasensitive detection of DNA is achieved via two-photon excitation of a fluorescent base analogue.

Two-photon fluorescence excitation in detection of biomolecules

2000 ◽  
Vol 28 (2) ◽  
pp. 70-74 ◽  
Author(s):  
E. Soini ◽  
N. J. Meltola ◽  
A. E. Soini ◽  
J. Soukka ◽  
J. T. Soini ◽  
...  
Keyword(s):  
Single Molecule ◽  
High Throughput Screening ◽  
Focal Point ◽  
Single Photons ◽  
Fluorescence Excitation ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Two Photon Excitation

Two-photon fluorescence excitation has been found to be a very powerful method for enhancing the sensitivity and resolution in far-field light microscopy. Two-photon fluorescence excitation also provides a substantially background-free detection on the single-molecule level. It allows direct monitoring of formation of labelled bio-molecule complexes in solution. Two-photon excitation is created when, by focusing an intensive light source, the density of photons per unit volume and per unit time becomes high enough for two photons to be absorbed into the same chromophore. In this case, the absorbed energy is the sum of the energies of the two photons. In two-photon excitation, dye molecules are excited only when both photons are absorbed simultaneously. The probability of absorption of two photons is equal to the product of probability distributions of absorption of the single photons. The emission of two photons is thus a quadratic process with respect to illumination intensity. Thus in two-photon excitation, only the fluorescence that is formed in the clearly restricted three-dimensional vicinity of the focal point is excited. We have developed an assay concept that is able to distinguish optically between the signal emitted from a microparticle in the focal point of the laser beam, and the signal emitted from the surrounding free labelled reagent. Moreover, the free labels outside the focal volume do not contribute any significant signal. This means that the assay is separation-free. The method based on two-photon fluorescence excitation makes possible fast single-step and separation-free immunoassays, for example, for whole blood samples. Since the method allows a separation-free assay in very small volumes, the method is very useful for high-throughput screening assays. Consequently we believe that two-photon fluorescence excitation will make a remarkable impact as a research tool and a routine method in many fields of analysis.

scholarly journals Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy

2016 ◽  
Vol 22 (21) ◽  
pp. 7281-7287 ◽  
Author(s):  
Stefania Impellizzeri ◽  
Sabrina Simoncelli ◽  
Gregory K. Hodgson ◽  
Anabel E. Lanterna ◽  
Christopher D. McTiernan ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation ◽  
Single Molecule Fluorescence Microscopy
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