Effect of Bin Time on the Photon Counting Histogram for One-Photon Excitation

ChemPhysChem ◽  
2005 ◽  
Vol 6 (5) ◽  
pp. 905-912 ◽  
Author(s):  
Thomas D. Perroud ◽  
Bo Huang ◽  
Richard N. Zare
Keyword(s):  
Photon Counting ◽  
Photon Excitation ◽  
Photon Counting Histogram

scholarly journals Photon Counting Histogram for One-Photon Excitation

ChemPhysChem ◽  
2003 ◽  
Vol 4 (12) ◽  
pp. 1280-1280 ◽  
Author(s):  
Thomas D. Perroud ◽  
Bo Huang ◽  
Mark I. Wallace ◽  
Richard N. Zare
Keyword(s):  
Photon Counting ◽  
Photon Excitation ◽  
Photon Counting Histogram

Photon Counting Histogram: One-Photon Excitation

ChemPhysChem ◽  
2004 ◽  
Vol 5 (10) ◽  
pp. 1523-1531 ◽  
Author(s):  
Bo Huang ◽  
Thomas D. Perroud ◽  
Richard N. Zare
Keyword(s):  
Photon Counting ◽  
Photon Excitation ◽  
Photon Counting Histogram

Photon Counting Histogram for One-Photon Excitation

ChemPhysChem ◽  
2003 ◽  
Vol 4 (10) ◽  
pp. 1121-1123 ◽  
Author(s):  
Thomas D. Perroud ◽  
Bo Huang ◽  
Mark I. Wallace ◽  
Richard N. Zare
Keyword(s):  
Photon Counting ◽  
Photon Excitation ◽  
Photon Counting Histogram

scholarly journals Exploring early time points of vimentin assembly in flow by fluorescence fluctuation spectroscopy

Lab on a Chip ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 735-745
Author(s):  
Eleonora Perego ◽  
Sarah Köster
Keyword(s):  
Reaction Kinetics ◽  
Photon Counting ◽  
Early Time ◽  
Fluorescence Fluctuation Spectroscopy ◽  
Microfluidic Mixing ◽  
Time Points ◽  
Photon Counting Histogram ◽  
Kinetics Of

The combination of photon counting histogram and microfluidic mixing reveals early time points in reaction kinetics of biomolecule aggregation.

A custom confocal and two-photon digital laser scanning microscope

2000 ◽  
Vol 278 (6) ◽  
pp. H2150-H2156 ◽  
Author(s):  
W. Gil Wier ◽  
C. William Balke ◽  
Jeffrey A. Michael ◽  
Joseph R. H. Mauban
Keyword(s):  
Laser Scanning ◽  
Photon Counting ◽  
Objective Lens ◽  
Scanning Microscope ◽  
Fluorescence Detector ◽  
Laser Scanning Microscope ◽  
Ti:Sapphire Laser ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

We describe a custom one-photon (confocal) and two-photon all-digital (photon counting) laser scanning microscope. The confocal component uses two avalanche photodiodes (APDs) as the fluorescence detector to achieve high sensitivity and to overcome the limited photon counting rate of a single APD (∼5 MHz). The confocal component is approximately nine times more efficient than our commercial confocal microscope (fluorophore fluo 4). Switching from one-photon to two-photon excitation mode (Ti:sapphire laser) is accomplished by moving a single mirror beneath the objective lens. The pulse from the Ti:sapphire laser is 109 fs in duration at the specimen plane, and average power is ∼5 mW. Two-photon excited fluorescence is detected by a fast photomultiplier tube. With a ×63 1.4 NA oil-immersion objective, the resolution of the confocal system is 0.25 μm laterally and 0.52 μm axially. For the two-photon system, the corresponding values are 0.28 and 0.82 μm. The system is advantageous when excitation intensity must be limited, when fluorescence is low, or when thick, scattering specimens are being studied (with two-photon excitation).

scholarly journals Dual-Color Photon Counting Histogram Analysis of mRFP1 and EGFP in Living Cells

2006 ◽  
Vol 91 (11) ◽  
pp. 4273-4284 ◽  
Author(s):  
Lindsey N. Hillesheim ◽  
Yan Chen ◽  
Joachim D. Müller
Keyword(s):  
Photon Counting ◽  
Living Cells ◽  
Histogram Analysis ◽  
Dual Color ◽  
Photon Counting Histogram

scholarly journals Amyloid Beta Oligomer Formation Analysis by Photon Counting Histogram

2009 ◽  
Vol 96 (3) ◽  
pp. 91a
Author(s):  
Naofumi Terada ◽  
Tamotsu Zako ◽  
Masafumi Sakono ◽  
Mizuo Maeda
Keyword(s):  
Amyloid Beta ◽  
Photon Counting ◽  
Oligomer Formation ◽  
Photon Counting Histogram
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