Fluorescence Spectroscopy of Single Molecules under Ambient Conditions: Methodology and Technology

ChemPhysChem ◽  
2003 ◽  
Vol 4 (8) ◽  
pp. 792-808 ◽  
Author(s):  
Martin Böhmer ◽  
Jörg Enderlein
Keyword(s):  
Fluorescence Spectroscopy ◽  
Single Molecules ◽  
Ambient Conditions

Multidimensional fluorescence spectroscopy of single molecules

2001 ◽  
Author(s):  
Claus Seidel ◽  
S. Berger ◽  
C. Eggeling ◽  
J. Schaffer ◽  
E. Schweinberger ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Single Molecules ◽  
Multidimensional Fluorescence

Characterization of Plasma-Modified Fluoropolymer Surfaces Using Steady-State and Time-Resolved Fluorescence Spectroscopy

1994 ◽  
Vol 48 (5) ◽  
pp. 630-637 ◽  
Author(s):  
Ming Li ◽  
Michaeleen L. Pacholski ◽  
Frank V. Bright
Keyword(s):  
Steady State ◽  
Fluorescence Spectroscopy ◽  
Sulfonyl Chloride ◽  
Sodium Dodecylsulfate ◽  
Covalent Immobilization ◽  
Ambient Conditions ◽  
Time Resolved Fluorescence ◽  
Decay Kinetics ◽  
Time Resolved

Poly(hexafluoropropylene-co-tetrafluoroethylene) (FEP) has been widely used in biotechnology because of its unique surface properties and biocompatibility. Recent work from our group has shown that plasma discharge-modified FEP can be used as the substratum for development of a very stable immunosensor. This result has prompted us to study further this new surface under ambient conditions. In this paper, we report on the covalent immobilization of a pyrene residue (-Py) onto FEP-APS (FEP-aminopropyl silane) surfaces and the characterization of FEP-APS-Py using steady-state and time-resolved fluorescence spectroscopy. Among the immobilization schemes tested, we found that the covalent coupling of pyrene-sulfonyl chloride to FEP-APS is the easiest and yields the most photostable FEP-APS-Py derivative. Steady-state emission spectra of FEP-APS-Py in contact with H2O, β-cyclodextrin (β-CD), and sodium dodecylsulfate (SDS) aqueous solutions differ considerably from those of Py-SO3 in solution. Time-resolved fluorescence spectroscopy of FEP-APS-Py demonstrates that the decay kinetics are strongly affected by the presence of ionic quenchers and molecular oxygen, as well as β-CD and SDS. The results are consistent with the suggestion that the APS-Py moiety undergoes a slow time-dependent reconfiguration at the FEP/APS interface.

Ultrafast dynamics of single molecules

2014 ◽  
Vol 43 (8) ◽  
pp. 2476-2491 ◽  
Author(s):  
Daan Brinks ◽  
Richard Hildner ◽  
Erik M. H. P. van Dijk ◽  
Fernando D. Stefani ◽  
Jana B. Nieder ◽  
...  
Keyword(s):  
Quantum Dynamics ◽  
Room Temperature ◽  
Organic Dyes ◽  
Single Molecules ◽  
Ultrafast Dynamics ◽  
Ambient Conditions ◽  
Vibrational Dynamics ◽  
Detailed Observation ◽  
Photosynthetic Complexes ◽  
And Control

Room-temperature studies of single molecules at femtosecond timescales provide detailed observation and control of ultrafast electronic and vibrational dynamics of organic dyes and photosynthetic complexes, probing quantum dynamics at ambient conditions and elucidating its role in chemistry and biology.

Quantitative Identification of Different Single Molecules by Selective Time-Resolved Confocal Fluorescence Spectroscopy

1998 ◽  
Vol 102 (33) ◽  
pp. 6601-6613 ◽  
Author(s):  
Joachim R. Fries ◽  
Leif Brand ◽  
Christian Eggeling ◽  
Malte Köllner ◽  
Claus A. M. Seidel
Keyword(s):  
Fluorescence Spectroscopy ◽  
Single Molecules ◽  
Time Resolved ◽  
Confocal Fluorescence ◽  
Quantitative Identification
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