Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood

2020 ◽  
Vol 93 (1) ◽  
Author(s):  
Jorge Bardina ◽  
Laura G. Rico ◽  
Michael D. Ward ◽  
Jolene A. Bradford ◽  
Jordi Juncà ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Whole Blood ◽  
Flow Cytometric

scholarly journals Effect of starvation and sampling time on plasma alkaline phosphatase activity and calcium homeostasis in the rat

1989 ◽  
Vol 23 (1) ◽  
pp. 53-58 ◽  
Author(s):  
C. S. Thompson ◽  
D. P. Mikhailidis ◽  
D. S. Gill ◽  
J. Y. Jeremy ◽  
J. L. Bell ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Whole Blood ◽  
Calcium Concentration ◽  
Sampling Time ◽  
Ionized Calcium ◽  
Plasma Calcium Concentration ◽  
Plasma Alkaline Phosphatase ◽  
25 Oh Vitamin D

The effect of starvation and sampling time on plasma alkaline phosphatase activity, total plasma calcium concentration and whole blood ionized calcium concentration was determined in the rat. Starvation caused a significant fall in total and ionized calcium concentrations as well as in alkaline phosphatase activity. These changes were accompanied by a fall in whole blood pH and an increase in the anion gap and a decrease in urinary excretion of calcium. These indices were restored to normal following refeeding. There was no change in serum 25-OH vitamin D concentrations following starvation for 3 days. Alkaline phosphatase activity showed a pattern compatible with the presence of a circadian rhythm when sampling took place between 0800 and 1800 h. Total and ionized calcium concentrations did not show such a rhythm when animals were fed the present diet.

scholarly journals Flow cytometric significance of cellular alkaline phosphatase activity in acute myeloid leukemia

Oncotarget ◽  
2019 ◽  
Vol 10 (65) ◽  
pp. 6969-6980
Author(s):  
Laura G. Rico ◽  
Jordi Juncà ◽  
Michael D. Ward ◽  
Jolene A. Bradford ◽  
Jordi Petriz
Keyword(s):  
Alkaline Phosphatase ◽  
Acute Myeloid Leukemia ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Myeloid Leukemia ◽  
Flow Cytometric ◽  
Acute Myeloid

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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