Analysis of Cellular DNA Content by Flow Cytometry

2017 ◽  
Vol 82 (1) ◽  
Author(s):  
Zbigniew Darzynkiewicz ◽  
Xuan Huang ◽  
Hong Zhao
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Cellular Dna

Flow cytometry, cellular DNA content, and prognosis in human malignancy.

1987 ◽  
Vol 5 (10) ◽  
pp. 1690-1703 ◽  
Author(s):  
D E Merkel ◽  
L G Dressler ◽  
W L McGuire
Keyword(s):  
Flow Cytometry ◽  
Data Base ◽  
Dna Content ◽  
Dna Analysis ◽  
Clinical Decision Making ◽  
Risk Groups ◽  
Clinical Decision ◽  
Tumor Type ◽  
Cellular Dna ◽  
Bladder Carcinomas

The use of flow cytometry to analyze the cellular DNA content of human malignancies has become increasingly commonplace. The relationship between abnormalities in DNA content or proliferative characteristics and prognosis is becoming clear for a variety of malignancies in part through new techniques that permit analysis of archival material. High- and low-risk groups of patients with early breast and bladder carcinomas, non-small-cell lung cancer, and colorectal, ovarian, and cervical carcinoma can be distinguished on the basis of abnormal stemline DNA content. In several hematologic and common pediatric malignancies, the prognostic relevance of DNA content flow cytometry has been similarly established. Though the interpretation of tumor cell cycle analyses is less certain, this characteristic may also be prognostically important. However, generalizations cannot be made when applying flow cytometric DNA analysis to clinical decision making. The prognostic importance of an abnormal DNA histogram for an individual patient must be assessed on the basis of the relevant data base for that particular tumor type. The current extent of this data base for various malignancies is reviewed.

scholarly journals Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.

1983 ◽  
Vol 31 (11) ◽  
pp. 1333-1335 ◽  
Author(s):  
D W Hedley ◽  
M L Friedlander ◽  
I W Taylor ◽  
C A Rugg ◽  
E A Musgrove
Keyword(s):  
Flow Cytometry ◽  
Retrospective Study ◽  
Clinical Outcome ◽  
Dna Content ◽  
Prognostic Significance ◽  
Distilled Water ◽  
Archival Material ◽  
Cellular Dna ◽  
Pathological Material ◽  
Dna Histograms

A method has been developed that allows flow cytometry to be used for measuring the cellular DNA content of paraffin-embedded human tumors. Thick (i.e., 30 micron) sections were cut from tissue blocks using a microtome and dewaxed in xylene. The sections were then rehydrated by sequentially immersing them in 100, 95, 70, and 50% ethanol before finally washing in distilled water. Single cell suspensions were then prepared by incubation in 0.5% pepsin, pH 1.5, at 37 degrees C for 30 min. The cells were counted, washed, and stained with 1 microgram/ml 4',6'-diamidino-2-phenylindole for 30 min, and DNA content was measured using an ICP 22 flow cytometer. There was a good correlation between the DNA histograms produced using this method and those obtained using unfixed tissue from the same tumor stained with ethidium bromide plus mithramycin. This method allows the retrospective study of archival material where the clinical outcome is already known, and it should, therefore, be particularly useful for determining the prognostic significance of abnormal DNA content measured by flow cytometry.

scholarly journals The coexistence of acute myeloblastic leukemia and diffuse histiocytic lymphoma in the same patient as demonstrated by multiparameter analysis

Blood ◽  
1979 ◽  
Vol 54 (6) ◽  
pp. 1428-1433
Author(s):  
DJ Straus ◽  
M Andreeff ◽  
HJ Hansen ◽  
R Mertelsmann ◽  
B Koziner ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Lymph Node ◽  
Dna Content ◽  
Acute Myeloblastic Leukemia ◽  
Histiocytic Lymphoma ◽  
Cellular Dna ◽  
Multiparameter Analysis ◽  
Diploid Cells ◽  
Diffuse Histiocytic Lymphoma

Measurement of cellular DNA content by flow cytometry demonstrated presence of two distinct aneuploid neoplasms in a patient who developed acute myeloblastic leukemia (AML) 4 mo after diagnosis of a diffuse histiocytic lymphoma (DHL). A lymph node aspirate contained peroxidase- negative, “null,” hyperdiploid (2.6C) DHL cells, while the bone marrow (BM) contained 84% primitive peroxidase-positive tetraploid AML cells (4.0C). Minor populations of hyperdiploid HDL and normal diploid cells could be detected by flow-cytometry in the BM, and all three populations were also seen in the peripheral blood.

Variation in DNA content among age classes of broad whitefish (Coregonus nasus) from the Sagavanirktok River delta

1991 ◽  
Vol 69 (5) ◽  
pp. 1335-1338 ◽  
Author(s):  
Samuel F. Lockwood ◽  
Brent T. Seavey ◽  
Robert E. Dillinger Jr. ◽  
John W. Bickham
Keyword(s):  
Flow Cytometry ◽  
Population Structure ◽  
Dna Content ◽  
River Delta ◽  
The Arctic ◽  
Age Classes ◽  
Entire Sample ◽  
Normal Distributions ◽  
Karyotypic Variability ◽  
Cellular Dna

Variation in cellular DNA content between age-classes was measured in 111 individuals of the Sagavanirktok River delta population of the broad whitefish, Coregonus nasus, using flow cytometry. Mean DNA content for the species was 7.07 pg. Deviations from normal distributions were found within age-class samples and for the entire sample. Intraspecific variation exceeding 75% of mean genome size, nearly twice that found in studies of other vertebrates, was most similar to that found in a study of coastal samples of a congener, the Arctic cisco (Coregonus autumnalis). This study illustrates the need for large sample sizes to account for karyotypic variability and population structure when reporting DNA content values for individual taxa.

Optimization of the conditions for simultaneous analysis of MYC nuclear oncoprotein expression and cellular DNA content by flow cytometry

1992 ◽  
Vol 76 (2) ◽  
pp. 243-243
Author(s):  
Pelisson Isabelle ◽  
Lizard Gérard ◽  
Mutin Mirielle ◽  
Chardonnet Yvette
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Simultaneous Analysis ◽  
Cellular Dna
Sign in / Sign up

Export Citation Format

Share Document