Quantifying the Proteolytic Cleavage of Plasma Membrane Proteins in Living Cells

2018 ◽  
Vol 81 (1) ◽  
pp. e58
Author(s):  
Martino Calamai ◽  
Francesco Saverio Pavone
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Proteolytic Cleavage ◽  
Living Cells ◽  
Plasma Membrane Proteins

scholarly journals Micropatterning of Plasma Membrane Proteins to Analyze Raft Localization in Living Cells

2010 ◽  
Vol 98 (3) ◽  
pp. 425a
Author(s):  
Julian Weghuber ◽  
Stefan Sunzenauer ◽  
Mario Brameshuber ◽  
Stefan Wieser ◽  
Lawrence Rajendran ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Living Cells ◽  
Plasma Membrane Proteins

Microfluidic fabrication of cell-derived nanovesicles as endogenous RNA carriers

Lab on a Chip ◽  
2014 ◽  
Vol 14 (7) ◽  
pp. 1261-1269 ◽  
Author(s):  
Wonju Jo ◽  
Dayeong Jeong ◽  
Junho Kim ◽  
Siwoo Cho ◽  
Su Chul Jang ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Living Cells ◽  
Plasma Membrane Proteins ◽  
Biological Signal

Artificial exosomes of ~100 nm diameter, enclosed with lipid bilayers, are fabricated from living cells and transfer biological signal components such as encapsulated RNAs and proteins, plasma membrane proteins, or both.

Detergent fractionation with subsequent subtractive suppression hybridization as a tool for identifying genes coding for plasma membrane proteins

2009 ◽  
Vol 18 (6) ◽  
pp. 527-535 ◽  
Author(s):  
Andreas Lange ◽  
Claudia Kistler ◽  
Tanja B. Jutzi ◽  
Alexandr V. Bazhin ◽  
Claus Detlev Klemke ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins ◽  
Subtractive Suppression Hybridization

scholarly journals pH-dependent Cargo Sorting from the Golgi

2011 ◽  
Vol 286 (12) ◽  
pp. 10058-10065 ◽  
Author(s):  
Chunjuan Huang ◽  
Amy Chang
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Atpase Activity ◽  
Secretory Pathway ◽  
Ph Dependence ◽  
Glucose Deprivation ◽  
Ph Homeostasis ◽  
Plasma Membrane Proteins ◽  
Cytosolic Ph ◽  
Cargo Sorting

The vacuolar proton-translocating ATPase (V-ATPase) plays a major role in organelle acidification and works together with other ion transporters to maintain pH homeostasis in eukaryotic cells. We analyzed a requirement for V-ATPase activity in protein trafficking in the yeast secretory pathway. Deficiency of V-ATPase activity caused by subunit deletion or glucose deprivation results in missorting of newly synthesized plasma membrane proteins Pma1 and Can1 directly from the Golgi to the vacuole. Vacuolar mislocalization of Pma1 is dependent on Gga adaptors although no Pma1 ubiquitination was detected. Proper cell surface targeting of Pma1 was rescued in V-ATPase-deficient cells by increasing the pH of the medium, suggesting that missorting is the result of aberrant cytosolic pH. In addition to mislocalization of the plasma membrane proteins, Golgi membrane proteins Kex2 and Vrg4 are also missorted to the vacuole upon loss of V-ATPase activity. Because the missorted cargos have distinct trafficking routes, we suggest a pH dependence for multiple cargo sorting events at the Golgi.

scholarly journals 5-Iodonaphthyl-1-azide labeling of plasma membrane proteins adjacent to specific sites via energy transfer

1997 ◽  
Vol 1324 (2) ◽  
pp. 320-332 ◽  
Author(s):  
Bruce I Meiklejohn ◽  
Noorulhuda A Rahman ◽  
Deborah A Roess ◽  
B.George Barisas
Keyword(s):  
Plasma Membrane ◽  
Energy Transfer ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins

scholarly journals Characterization of Plasma Membrane Proteins in Mammalian Kidney

1969 ◽  
Vol 244 (13) ◽  
pp. 3561-3569
Author(s):  
D F Fitzpatrick ◽  
G R Davenport ◽  
L Forte ◽  
E J Landon
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Plasma Membrane Proteins ◽  
Mammalian Kidney
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