scholarly journals Characterization of RNA‐binding motif 3 (RBM3) protein levels and nuclear architecture changes in aggressive and recurrent prostate cancer

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Neil M. Carleton ◽  
Guangjing Zhu ◽  
M. Craig Miller ◽  
Christine Davis ◽  
Prakash Kulkarni ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Rna Binding ◽  
Nuclear Architecture ◽  
Binding Motif ◽  
Recurrent Prostate Cancer ◽  
Protein Levels

Characterization of fibroblast-free CWR-R1ca castration-recurrent prostate cancer cell line

The Prostate ◽  
2016 ◽  
Vol 76 (12) ◽  
pp. 1067-1077 ◽  
Author(s):  
Mojgan Shourideh ◽  
Adam DePriest ◽  
James L. Mohler ◽  
Elizabeth M. Wilson ◽  
Shahriar Koochekpour
Keyword(s):  
Prostate Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Line ◽  
Prostate Cancer Cell Line ◽  
Recurrent Prostate Cancer

High RNA-binding motif protein 3 expression is an independent prognostic marker in operated prostate cancer and tightly linked to ERG activation and PTEN deletions

2014 ◽  
Vol 50 (4) ◽  
pp. 852-861 ◽  
Author(s):  
Katharina Grupp ◽  
Julia Wilking ◽  
Kristina Prien ◽  
Claudia Hube-Magg ◽  
Hüseyin Sirma ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Prognostic Marker ◽  
Rna Binding ◽  
Binding Motif ◽  
Independent Prognostic Marker

scholarly journals Systematic characterization of branch point binding protein, splicing factor 1, gene family in plant development and stress responses

2019 ◽  
Author(s):  
Kai-Lu Zhang ◽  
Zhen Feng ◽  
Jing-Fang Yang ◽  
Tian Yuan ◽  
Di Zhang ◽  
...  
Keyword(s):  
Gene Family ◽  
Plant Species ◽  
Stress Responses ◽  
Messenger Rna ◽  
Rna Binding ◽  
Binding Protein ◽  
Splicing Factor ◽  
Binding Motif ◽  
Splicing Factor 1

Abstract Background: Among eukaryotic organisms, the splicing of nuclear precursor messenger RNA (pre-mRNA) is a process of introns excision and sequentially joining of exons, leading multi-exonic genes to generate multiple splicing isoforms at transcription level. This process is carried out by a super-protein complex defined as spliceosome. Specifically, splicing factor 1/branchpoint binding protein (SF1/BBP) is a single protein that can bind to the intronic branchpoint sequence (BPS), connecting 5’ and 3’ splice site binding complexes during early spliceosome assembly. The molecular function of this protein has been extensively investigated in yeast, metazoan and mammals. However, their counterparts in plants are seldomly reported. Results: Here, we conducted a systematic characterization of SF1 gene family across plant lineage. In this work, a total of 92 sequences from 59 plant species were identified. Phylogenetic relationships of these sequences were constructed and subsequent bioinformatic analysis suggested that this family is likely originated from an ancient gene transposition duplication event. Most plant species were shown to maintain a single copy of this gene. Furthermore, an additional RNA binding motif (RRM) existed in most members of this gene family in comparison to their animal and yeast counterparts, indicating their potential role conserved in plant lineage. Conclusion: Our comprehensive analysis presents general feature of gene and protein structure of this splicing factor family and will provide fundamental information for further functional studies in plants.

scholarly journals Comprehensive Characterization of Androgen-Responsive circRNAs in Prostate Cancer

Life ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1096
Author(s):  
Zhe Kong ◽  
Yali Lu ◽  
Xuechao Wan ◽  
Jun Luo ◽  
Dujian Li ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Molecular Mechanisms ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Circular Rnas ◽  
Transcriptional Level ◽  
The Novel ◽  
Cancer Tissues ◽  
Posttranscriptional Level

The androgen receptor (AR) signaling pathway plays an important role in the initiation and progression of prostate cancer. Circular RNAs (circRNAs), the novel noncoding RNAs without 5′ to 3′ polarity or 3′ poly (A), play an important role in multiple diseases. However, the potential roles of androgen-responsive circRNAs in prostate cancer remain unclear. In this study, we identified 3237 androgen-responsive circRNAs and 1954 androgen-responsive mRNAs after dihydrotestosterone (DHT) stimulation using microarray. Among them, the expression of 1296 androgen-responsive circRNAs was consistent with that of their parent genes, and we thought AR might regulate the expression of these circRNAs at the transcriptional level. In addition, 1941 circRNAs expression was not consistent with their parent genes, and we speculated that AR may regulate the expression of those circRNAs at the posttranscriptional level through affecting alternative splicing. Analyzing the androgen-responsive circRNAs regulated at the posttranscriptional level, we identified two key RNA binding proteins (RBPs), WTAP and TNRC6, using the circInteractome database, which may play important role in the biogenesis of androgen-responsive circRNAs. Furthermore, we explored the potential biological functions and predicted the molecular mechanisms of two dysregulated circRNAs (circNFIA and circZNF561) in prostate cancer. In this study, we revealed that circNFIA was upregulated in prostate cancer tissues and plasma samples from patients with prostate cancer; circNFIA may play an oncogenic role in prostate cancer. In contrast, circZNF561 was downregulated and may act as a tumor suppressor in prostate cancer. Our results suggest that androgen-responsive circRNAs might regulate the progression of prostate cancer and could be novel diagnostic biomarkers.

scholarly journals Processing Body Formation Limits Proinflammatory Cytokine Synthesis in Endotoxin-Tolerant Monocytes and Murine Septic Macrophages

2015 ◽  
Vol 7 (6) ◽  
pp. 572-583 ◽  
Author(s):  
Clara McClure ◽  
Laura Brudecki ◽  
Zhi Q. Yao ◽  
Charles E. McCall ◽  
Mohamed El Gazzar
Keyword(s):  
Protein Synthesis ◽  
Rna Binding ◽  
Mrna Translation ◽  
Endotoxin Tolerance ◽  
Translational Repression ◽  
Binding Motif ◽  
Body Formation ◽  
Translational Repressor ◽  
Protein Levels ◽  
Repressor Complex

An anti-inflammatory phenotype with pronounced immunosuppression develops during sepsis, during which time neutrophils and monocytes/macrophages limit their Toll-like receptor 4 responses to bacterial lipopolysaccharide (LPS/endotoxin). We previously reported that during this endotoxin-tolerant state, distinct signaling pathways differentially repress transcription and translation of proinflammatory cytokines such as TNFα and IL-6. Sustained endotoxin tolerance contributes to sepsis mortality. While transcription repression requires chromatin modifications, a translational repressor complex of Argonaute 2 (Ago2) and RNA-binding motif protein 4 (RBM4), which bind the 3′-UTR of TNFα and IL-6 mRNA, limits protein synthesis. Here, we show that Dcp1 supports the assembly of the Ago2 and RBM4 repressor complex into cytoplasmic processing bodies (p-bodies) in endotoxin-tolerant THP-1 human monocytes following stimulation with LPS, resulting in translational repression and limiting protein synthesis. Importantly, this translocation process is reversed by Dcp1 knockdown, which restores TNFα and IL-6 protein levels. We also find this translational repression mechanism in primary macrophages of septic mice. Because p-body formation is a critical step in mRNA translation repression, we conclude that Dcp1 is a major component of the translational repression machinery of endotoxin tolerance and may contribute to sepsis outcome.

scholarly journals The master transcription factor SOX2, mutated in anophthalmia/microphthalmia, is post-transcriptionally regulated by the conserved RNA-binding protein RBM24 in vertebrate eye development

2019 ◽  
Vol 29 (4) ◽  
pp. 591-604 ◽  
Author(s):  
Soma Dash ◽  
Lindy K Brastrom ◽  
Shaili D Patel ◽  
C Anthony Scott ◽  
Diane C Slusarski ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Control ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Eye Development ◽  
Ocular Tissue ◽  
Binding Motif ◽  
Mobility Shift ◽  
Developmental Defects ◽  
Protein Levels

Abstract Mutations in the key transcription factor, SOX2, alone account for 20% of anophthalmia (no eye) and microphthalmia (small eye) birth defects in humans—yet its regulation is not well understood, especially on the post-transcription level. We report the unprecedented finding that the conserved RNA-binding motif protein, RBM24, positively controls Sox2 mRNA stability and is necessary for optimal SOX2 mRNA and protein levels in development, perturbation of which causes ocular defects, including microphthalmia and anophthalmia. RNA immunoprecipitation assay indicates that RBM24 protein interacts with Sox2 mRNA in mouse embryonic eye tissue. and electrophoretic mobility shift assay shows that RBM24 directly binds to the Sox2 mRNA 3’UTR, which is dependent on AU-rich elements (ARE) present in the Sox2 mRNA 3’UTR. Further, we demonstrate that Sox2 3’UTR AREs are necessary for RBM24-based elevation of Sox2 mRNA half-life. We find that this novel RBM24–Sox2 regulatory module is essential for early eye development in vertebrates. We show that Rbm24-targeted deletion using a constitutive CMV-driven Cre in mouse, and rbm24a-CRISPR/Cas9-targeted mutation or morpholino knockdown in zebrafish, results in Sox2 downregulation and causes the developmental defects anophthalmia or microphthalmia, similar to human SOX2-deficiency defects. We further show that Rbm24 deficiency leads to apoptotic defects in mouse ocular tissue and downregulation of eye development markers Lhx2, Pax6, Jag1, E-cadherin and gamma-crystallins. These data highlight the exquisite specificity that conserved RNA-binding proteins like RBM24 mediate in the post-transcriptional control of key transcription factors, namely, SOX2, associated with organogenesis and human developmental defects.

scholarly journals Prognostic value and oncogenic effect of increased RBM8A expression in colon adenocarcinoma

2021 ◽  
Author(s):  
Zhongpeng Xie ◽  
Yu Wu ◽  
Yahui Huang ◽  
Yuxiang Cai ◽  
Yanyan Wang ◽  
...  
Keyword(s):  
Rna Binding ◽  
Colon Adenocarcinoma ◽  
Clinicopathological Characteristics ◽  
Survival Prediction ◽  
Binding Motif ◽  
Depth Of Invasion ◽  
Protein Levels

RNA Binding Motif Protein 8A (RBM8A) is a component of the spliceosome, which couples pre- and post-mRNA splicing events. Dysregulated expression of RBM8A has been recently discovered in hepatocellular carcinoma and gastric cancer. This study aimed to investigate the expression pattern of RBM8A protein in colon adenocarcinoma and to explore its correlation with patients’ clinicopathological characteristics as well as clinical outcomes. Accordingly, we initially found that RBM8A was significantly higher in colon adenocarcinoma tissues compared to adjacent tissues on both mRNA and protein levels. Meanwhile, RBM8A protein was positively correlated with tumor size, depth of invasion, and lymph node metastasis. Furthermore, patients with increased RBM8A expression or positive lymph nodes exhibited a poorer overall survival. Consistently, immunoblotting data showed that RBM8A was higher in SW480 and SW620 colon adenocarcinoma cell lines compared to nontumorous cells. Finally, in vitro and in vivo assays elucidated the role of RBM8A on promoting colon cancer growth by using knockdown strategy. Taken together, our study demonstrated that RBM8A may act as a proto-oncogene, which could be a promising biomarker and therapeutic target in the survival prediction and treatment of colon adenocarcinoma.

Sign in / Sign up

Export Citation Format

Share Document