In situ hybridization analysis of vasopressin gene transcription in the paraventricular and supraoptic nuclei of the rat: Regulation by stress and glucocorticoids

1995 ◽  
Vol 363 (1) ◽  
pp. 15-27 ◽  
Author(s):  
James P. Herman
Keyword(s):  
In Situ Hybridization ◽  
Gene Transcription ◽  
Hybridization Analysis ◽  
Vasopressin Gene ◽  
Supraoptic Nuclei

Effects of acute hypotensive stimuli on arginine vasopressin gene transcription in the rat hypothalamus

2000 ◽  
Vol 279 (4) ◽  
pp. E886-E892 ◽  
Author(s):  
Satoshi Kakiya ◽  
Hiroshi Arima ◽  
Hisashi Yokoi ◽  
Takashi Murase ◽  
Yuko Yambe ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mean Arterial Pressure ◽  
Arterial Pressure ◽  
Gene Transcription ◽  
Arginine Vasopressin ◽  
The Other ◽  
Paraventricular Nuclei ◽  
Vasopressin Gene ◽  
Avp Gene

We investigated the baroregulation of arginine vasopressin (AVP) gene transcription in the supraoptic (SON) and paraventricular nuclei (PVN) in conscious rats by use of intronic in situ hybridization. Hemorrhage of 16 ml/kg body wt decreased mean arterial pressure (MAP) by 57% and increased both plasma AVP (control, 1.2 ± 0.3 pg/ml; 16 ml/kg body wt, 38.9 ± 3.2 pg/ml) at 10 min and AVP heteronuclear (hn)RNA levels (SON, 150%; PVN, 140% of control values) at 20 min. On the other hand, hemorrhage of 7 ml/kg body wt had no significant effect on MAP, plasma AVP, or the AVP hnRNA levels. To better understand the baroregulation, we also examined the effects of sodium nitroprusside (SNP), which induces hypotension without a change in blood volume. The subcutaneous injection of 2 mg/kg body wt SNP, which decreased the MAP by 60%, increased both plasma AVP (control, 1.6 ± 0.4 pg/ml; 2 mg/kg body wt, 8.1 ± 0.4 pg/ml) at 10 min and AVP hnRNA levels (SON, 150%; PVN, 140% of control values) at 30 min. The injection of 0.1 mg/kg body wt SNP, which reduced the MAP by 10%, failed to increase either the plasma AVP or AVP hnRNA levels. These results indicate that AVP gene transcription increases rapidly after both hypotensive hemorrhage and normovolemic hypotension. In addition, it is suggested that the set point for AVP synthesis in the baroregulation is similar to that for AVP release.

scholarly journals Fluorescence in situ hybridization analysis of hindgut bacteria associated with the development of equine laminitis

2008 ◽  
Vol 10 (10) ◽  
pp. 2901-2901 ◽  
Author(s):  
Gabriel J. Milinovich ◽  
Darren J. Trott ◽  
Paul C. Burrell ◽  
Emma L. Croser ◽  
Rafat A. M. Al Jassim ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Hybridization Analysis

Fluorescence In Situ Hybridization Analysis of Sex-Chromosome Mosaicism in Azoospermic Men

2001 ◽  
Vol 22 (6) ◽  
pp. 970-972 ◽  
Author(s):  
HIROSHI OKADA ◽  
MASAKI DOBASHI ◽  
TAKAFUMI YAMAZAKI ◽  
MASATO FUJISAWA ◽  
SOICHI ARAKAWA ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sex Chromosome ◽  
Hybridization Analysis ◽  
Chromosome Mosaicism

scholarly journals Locked Nucleic Acid In situ Hybridization Analysis of miR-21 Expression during Colorectal Cancer Development

2009 ◽  
Vol 15 (12) ◽  
pp. 4009-4016 ◽  
Author(s):  
Nobutake Yamamichi ◽  
Ryoichi Shimomura ◽  
Ken-ichi Inada ◽  
Kouhei Sakurai ◽  
Takeshi Haraguchi ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Hybridization Analysis ◽  
Locked Nucleic Acid ◽  
Cancer Development

Diagnosis of aneuploidy by in situ hybridization: Analysis of interphase nuclei

1991 ◽  
Vol 112 (4) ◽  
pp. 1480-1483 ◽  
Author(s):  
S. G. Vorsanova ◽  
Yu. B. Yurov ◽  
G. V. Deryagin ◽  
I. V. Solov'ev ◽  
G. A. Bytenskaya
Keyword(s):  
In Situ Hybridization ◽  
Hybridization Analysis ◽  
Interphase Nuclei

scholarly journals P3.03-017 Fluorescent in situ Hybridization Analysis of MET Gene Status in Malignant Mesothelioma

2017 ◽  
Vol 12 (1) ◽  
pp. S1354
Author(s):  
Antonella Vigani ◽  
Sandra Salvi ◽  
Serena Varesano ◽  
Simona Boccardo ◽  
Paola Ferro ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Malignant Mesothelioma ◽  
Fluorescent In Situ Hybridization ◽  
Hybridization Analysis ◽  
Gene Status
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