In situ hybridization detection of trka mRNA in brain: Distribution, colocalization with p75NGFR and up-regulation by nerve growth factor

1994 ◽  
Vol 341 (3) ◽  
pp. 324-339 ◽  
Author(s):  
Robert B. Gibbs ◽  
D. W. Pfaff
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Brain Distribution ◽  
Nerve Growth ◽  
Hybridization Detection

scholarly journals Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth Factor

1997 ◽  
Vol 45 (6) ◽  
pp. 795-804 ◽  
Author(s):  
Hooman Farhadi ◽  
Sangeeta Pareek ◽  
Robert Day ◽  
Weijia Dong ◽  
Michel Chrétien ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Proteolytic Enzymes ◽  
Mrna Levels ◽  
Prohormone Convertases ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Ductal Cells

Nerve growth factor (NGF) in mouse submandibular glands (SGs) is generated from a 35-kD precursor by proteolytic enzymes that have yet to be identified. Prohormone convertases (PCs) cleave the NGF precursor in vitro, and in this study we questioned whether PCs could process salivary NGF in vivo. mRNA coding for PC2 (but not PC1) was detected on Northern blots of SG mRNA and also by in situ hybridization within parasympathetic neurons of intralobular ganglia. Northern blot and in situ hybridization analyses also detect mRNA coding for furin. In SGs of male mice, furin mRNA levels are high at birth and remain high throughout development. In glands from female mice, levels decline during postnatal development and are lower in adults than in newborns. Immunocytochemistry detects furin immunoreactivity in pro-acinar and ductal cells of glands from newborn and pubescent mice. In glands of adults, furin immunoreactivity is detectable in acinar cells but highest levels are present in NGF-containing granular convoluted tubule cells. These data, taken together with those from previous studies, suggest that furin is a candidate processing enzyme for NGF in mouse submandibular glands.

scholarly journals Detection of nerve growth factor mRNA in rodent salivary glands with digoxigenin- and 33P-labeled oligonucleotides: effects of castration and sympathectomy.

1993 ◽  
Vol 41 (5) ◽  
pp. 703-708 ◽  
Author(s):  
C Humpel ◽  
E Lindqvist ◽  
L Olson
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Male Mouse ◽  
Male Mice ◽  
Nerve Growth ◽  
Submandibular Glands

Nerve growth factor (NGF) is a protein highly expressed in the male mouse submandibular gland. We have applied a non-radioactive in situ hybridization method using digoxigenin-labeled NGF oligonucleotides, and have found the highest amounts of NGF mRNA in the secretory striated ducts of the male mouse submandibular gland. Scattered strongly positive cells were found in male mouse sublingual glands. Weakly labeled cells were seen in female mouse and in male rat submandibular gland striated duct cells. Using 33P as an alternative to 32P and 35S, we demonstrated a 1.3 KB NGF mRNA in salivary glands of male mice by Northern blot hybridization. Using 33P we detected NGF mRNA in male mouse submandibular glands by in situ hybridization but with a signal that, compared with the non-radioactive method, had a very low resolution. Castration of male mice almost abolished both the 1.3 KB NGF mRNA seen with Northern blots and the NGF mRNA labeling in submandibular glands 4 weeks after the operation, whereas levels were increased 6 hr and 2 days after sympathectomy. We conclude that hybridization with digoxigenin-labeled NGF oligonucleotides is a good tool to study the expression and regulation of NGF mRNA in male mouse submandibular glands.

Assignment of the beta-nerve growth factor (NGFB) to bovine chromosome 3 band q23 by in situ hybridization

1997 ◽  
Vol 77 (3-4) ◽  
pp. 306-307 ◽  
Author(s):  
C. Elduque ◽  
P. Laurent ◽  
H. Hayes ◽  
C. Rodellar ◽  
H. Levéziel ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Bovine Chromosome ◽  
Chromosome 3 ◽  
Nerve Growth

scholarly journals Nerve growth factor- and epidermal growth factor-stimulated phosphorylation of a PC12 cytoskeletally associated protein in situ.

1985 ◽  
Vol 100 (3) ◽  
pp. 677-683 ◽  
Author(s):  
G E Landreth ◽  
G D Rieser
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Pc12 Cells ◽  
Receptor Occupancy ◽  
Hormone Action ◽  
Intact Cells ◽  
Nerve Growth ◽  
Epidermal Growth

Nerve growth factor (NGF) and epidermal growth factor (EGF) produce stable alterations in PC12 cells that persist in the detergent-insoluble cytoskeleton, resulting in the phosphorylation of a 250,000-mol-wt cytoskeletally associated protein in situ. Treatment of PC12 cells with NGF or EGF, followed by detergent lysis of the cells and incubation of the resulting cytoskeletons with gamma-32P-ATP, permitted detection of hormonally stimulated, energy-dependent events, which result in the enhanced phosphorylation of a cytoskeletally associated protein as an immediate consequence of receptor occupancy. These events were elicited only upon treatment of intact cells at physiological temperatures. The NGF- and EGF-stimulated events occurred rapidly; however, they were a transient effect of hormone action. NGF and EGF were found to act through independent mechanisms to stimulate the in situ phosphorylation of the 250,000-mol-wt protein, as the effects of NGF, but not EGF, were blocked by methyltransferase inhibitors. The 250,000-mol-wt protein was phosphorylated on serine and threonine residues in response to both NGF and EGF although in somewhat different proportions. The data suggest that the hormone-stimulated labeling of the 250,000-mol-wt protein may be the result of either the direct activation of a protein kinase, the redistribution of the kinase relative to its substrates as a consequence of hormone action, or the coincident occurrence of these events.

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