Co-localization of retrogradely transported wheat germ agglutinin and the putative neurotransmitter substance P within trigeminal ganglion cells projecting to cat middle cerebral artery

1984 ◽  
Vol 225 (2) ◽  
pp. 187-192 ◽  
Author(s):  
Lee-Yuan Liu-Chen ◽  
Steven A. Gillespie ◽  
Thorkild V. Norregaard ◽  
Michael A. Moskowitz
Keyword(s):  
Substance P ◽  
Middle Cerebral Artery ◽  
Trigeminal Ganglion ◽  
Cerebral Artery ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Ganglion Cells ◽  
Neurotransmitter Substance

High serum substance P levels and mortality after malignant middle cerebral artery infarction

2020 ◽  
Vol 57 ◽  
pp. 1-4 ◽  
Author(s):  
Leonardo Lorente ◽  
María M. Martín ◽  
Antonia Pérez-Cejas ◽  
Agustín F. González-Rivero ◽  
Mónica Argueso ◽  
...  
Keyword(s):  
Substance P ◽  
Middle Cerebral Artery ◽  
Cerebral Artery ◽  
High Serum ◽  
Middle Cerebral Artery Infarction

scholarly journals Investigation of Postmortem Functional Changes in Human Cerebral Arteries

1993 ◽  
Vol 13 (2) ◽  
pp. 346-349 ◽  
Author(s):  
Hisashi Onoue ◽  
Nobuyoshi Kaito ◽  
Shogo Tokudome ◽  
Toshiaki Abe ◽  
Koichi Tashibu ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Substance P ◽  
Middle Cerebral Artery ◽  
Cerebral Artery ◽  
Vascular Endothelium ◽  
Cerebral Arteries ◽  
Time Dependent ◽  
Functional Changes ◽  
Human Cadavers

This study demonstrated the time-dependent changes in postmortem responses of isolated human middle cerebral artery strips to vasodilators. The relaxation induced by prostaglandin (PG) I2 or nitroglycerin remained stable for 24 h postmortem. In arterial strips precontracted with PGF2α, substance P and bradykinin both elicited relaxation that was almost completely abolished by removal of the endothelium. The endothelium-dependent response to both peptides was significantly degraded in strips obtained >12 h postmortem. These results indicate a selective functional or anatomical vulnerability of the vascular endothelium compared with that of the vasodilator mechanisms of the smooth muscle in the postmortem period. However, cerebral arteries isolated from human cadavers within 12 h postmortem should be adequate for studies of both smooth muscle and endothelial reactivity to vasodilators.

scholarly journals Retrograde Tracing of Nerve Fibers to the Rat Middle Cerebral Artery with True Blue: Colocalization with Different Peptides

1989 ◽  
Vol 9 (2) ◽  
pp. 212-218 ◽  
Author(s):  
L. Edvinsson ◽  
H. Hara ◽  
R. Uddman
Keyword(s):  
Dorsal Root Ganglion ◽  
Middle Cerebral Artery ◽  
Nerve Cell ◽  
Trigeminal Ganglion ◽  
Cerebral Artery ◽  
Superior Cervical Ganglion ◽  
Dorsal Root ◽  
Nerve Fibers ◽  
Retrograde Tracing ◽  
Cervical Ganglion

The origin of nerve fibers to the rat middle cerebral artery was studied by retrograde tracing with the fluorescent tracer True Blue (TB) in combination with immunocytochemistry to known perivascular peptides. Application of TB to the middle cerebral artery labeled nerve cell bodies in the ipsilateral superior cervical ganglion, the otic ganglion, the sphenopalatine ganglion, the trigeminal ganglion, and the cervical dorsal root ganglion at level C2. A few labeled nerve cell bodies were seen in contralateral ganglia. Judging from the number and intensity of the labeling, the superior cervical ganglion and the trigeminal ganglion and dorsal root ganglion at level C2 contributed most to the innervation. A moderate number of nerve cell bodies were labeled in the sphenopalatine and otic ganglia. The TB-labeled nerve cell bodies were further examined for the presence of neuropeptides. For that purpose antibodies raised against neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), substance P (SP) and calcitonin gene-related peptide (CGRP) were used. A considerable portion of the TB-labeled nerve cell bodies in the superior cervical ganglion contained NPY. About half of the labeled nerve cell bodies in the sphenopalatine and otic ganglia contained VIP. In the trigeminal ganglion and in the dorsal root ganglion at level C2, one-third of the TB-labeled nerve cell bodies were CGRP-immunoreactive, while only few nerve cell bodies contained SP. The study provides direct evidence for the origin of cerebrovascular peptidergic nerve fibers and demonstrates that not only ipsilateral but also contralateral ganglia contribute to the innervation of the cerebral circulation.

scholarly journals Localization of axonally transported 125I-wheat germ agglutinin beneath the plasma membrane of chick retinal ganglion cells.

1983 ◽  
Vol 96 (2) ◽  
pp. 373-381 ◽  
Author(s):  
J H LaVail ◽  
I K Sugino ◽  
D M McDonald
Keyword(s):  
Plasma Membrane ◽  
Retinal Ganglion Cells ◽  
Optic Tectum ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Plasma Membranes ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Electron Microscopic Autoradiography ◽  
Neuron Cell Body

The distribution of 125I-wheat germ agglutinin (WGA) transported by axons of chick retinal ganglion cells to layer d of the optic tectum was studied by electron microscopic autoradiography. We found that 52% of the radioactivity was located in axons and axon terminals in the contralateral optic tectum 22 h after intravitreal injection of affinity-purified 125I-WGA. Axons comprised 43% of the volume of layer d. Dendrites, glial cells, and neuron cell bodies contained 20%, 17%, and 3% of the label, whereas these structures comprised 24%, 21%, and 2% of the tissue volume, respectively. We also measured the distances between the autoradiographic silver grains and the plasma membranes of these profiles, and compared observed distributions of grains to theoretical distributions computed for band-shaped sources at various distances from the plasma membranes. This analysis revealed that the radioactive source within axons was distributed in a band of cytoplasm extending in from the plasma membrane a distance of 63 nm. Because WGA is known to bind to specific membrane glycoconjugates, we infer that at least some glycoconjugates may be concentrated within an annular region of cytoplasm just beneath the axonal plasma membrane after axoplasmic transport from the neuron cell body.

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