Distribution of cholinergic neurons in rat brain: Demonstrated by the immunocytochemical localization of choline acetyltransferase

1983 ◽  
Vol 216 (1) ◽  
pp. 53-68 ◽  
Author(s):  
David M. Armstrong ◽  
Clifford B. Saper ◽  
Allan I. Levey ◽  
Bruce H. Wainer ◽  
Robert D. Terry
Keyword(s):  
Rat Brain ◽  
Choline Acetyltransferase ◽  
Cholinergic Neurons ◽  
Immunocytochemical Localization

Ultrastructural Localization of Acetylcholinesterase in the Arcuate Nucleus and Median Eminence of the Rat

1977 ◽  
Vol 35 ◽  
pp. 440-441
Author(s):  
K.A. Carson ◽  
C.B. Nemeroff ◽  
M.S. Rone ◽  
J.S. Kizer ◽  
J.S. Hanker
Keyword(s):  
Choline Acetyltransferase ◽  
Median Eminence ◽  
Arcuate Nucleus ◽  
Cholinergic Neurons ◽  
Ultrastructural Localization ◽  
Male Rats ◽  
Neonatal Rats ◽  
Good Marker ◽  
Chemical Studies ◽  
High Doses

Biochemical, physiological, pharmacological, and more recently enzyme histo- chemical data have indicated that cholinergic circuits exist in the hypothalamus. Ultrastructural correlates of these pathways such as acetylcholinesterase (AchE) positive neurons in the arcuate nucleus (ARC) and stained terminals in the median eminence (ME) have yet to be described. Initial studies in our laboratories utilizing chemical lesioning and microdissection techniques coupled with microchemical and light microscopic enzyme histo- chemical studies suggested the existence of cholinergic neurons in the ARC which project to the ME (1). Furthermore, in adult male rats with Halasz deafferentations (hypothalamic islands composed primarily of the isolated ARC and the ME) choline acetyltransferase (ChAc) activity, a good marker for cholinergic neurons, was not significantly reduced in the ME and was only somewhat reduced in the ARC (2). Treatment of neonatal rats with high doses of monosodium 1-glutamate (MSG) results in a lesion largely restricted to the neurons of the ARC.

Immunocytochemical Localization of 5α-Reductase in Rat Brain

1994 ◽  
Vol 5 (5) ◽  
pp. 394-399 ◽  
Author(s):  
Georges Pelletier ◽  
Van Luu-The ◽  
Fernand Labrie
Keyword(s):  
Rat Brain ◽  
Immunocytochemical Localization

Immunocytochemical Localization of Thyroid Hormone Receptors in the Adult Rat Brain

Thyroid ◽  
1991 ◽  
Vol 1 (2) ◽  
pp. 173-184 ◽  
Author(s):  
J. PUYMIRAT ◽  
M. MIEHE ◽  
R. MARCHAND ◽  
L. SARLIEVE ◽  
J.H. DUSSAULT
Keyword(s):  
Thyroid Hormone ◽  
Rat Brain ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptors ◽  
Immunocytochemical Localization ◽  
Adult Rat ◽  
Adult Rat Brain

Immunocytochemical localization of enkephalin in the neostriatum of rat brain: A light and electron microscopic study

1980 ◽  
Vol 189 (4) ◽  
pp. 721-740 ◽  
Author(s):  
Virginia M. Pickel ◽  
Khushdev K. Sumal ◽  
Susan C. Beckley ◽  
Richard J. Miller ◽  
Donald J. Reis
Keyword(s):  
Rat Brain ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Immunocytochemical Localization ◽  
Electron Microscopic

Effects of altered cholinergic function on working and reference memory in the rat

1986 ◽  
Vol 64 (3) ◽  
pp. 376-382 ◽  
Author(s):  
Richard J. Beninger ◽  
B. A. Wirsching ◽  
Khem Jhamandas ◽  
Roland J. Boegman ◽  
Sherif R. El-Defrawy
Keyword(s):  
Working Memory ◽  
Choline Acetyltransferase ◽  
Radial Maze ◽  
Cholinergic Neurons ◽  
Reference Memory ◽  
Nucleus Basalis ◽  
Nucleus Basalis Magnocellularis ◽  
Memory Errors ◽  
Biochemical Assays ◽  
Alternation Task

Many data suggest that the brain's cholinergic neurons participate in the control of memory and it has been suggested that cholinergic systems are involved differentially in working and reference memory. To test this hypothesis the effects on memory of unilateral injections of the neurotoxins, quinolinic acid or kainic acid into the cortically projecting cholinergic cells of the nucleus basalis magnocellularis (nbm) were evaluated. In experiment 1, quinolinate-injected (n = 7) and sham-operated (n = 7) rats were tested in a T-maze alternation task that requires working memory. Lesion rats performed significantly more poorly than shams and subsequent biochemical assays of cortical choline acetyltransferase (CAT) activity revealed significant reductions in the lesion rats. In experiment 2, kainate-injected (n = 9) and sham-operated (n = 8) rats were trained in an eight-arm radial maze with only four arms baited. Lesion rats made significantly more working memory errors (entries into baited arms from which the food had already been collected) than reference memory errors (entries into never baited arms). CAT assays showed that the lesion led to a decrease in cortical CAT with no significant change in hippocampal CAT. The results of these studies support the hypothesis that cholinergic neurons of the basocortical system may be differentially involved in working and reference memory.

Sign in / Sign up

Export Citation Format

Share Document