Intracellular marking with Lucifer Yellow CH and horseradish peroxidase of cells electrophysiologically characterized as glia in the cerebral cortex of the cat

1979 ◽  
Vol 186 (2) ◽  
pp. 173-188 ◽  
Author(s):  
Michiaki Takato ◽  
Sidney Goldring
Keyword(s):  
Cerebral Cortex ◽  
Horseradish Peroxidase ◽  
Lucifer Yellow ◽  
Lucifer Yellow Ch

scholarly journals Phorbol esters and horseradish peroxidase stimulate pinocytosis and redirect the flow of pinocytosed fluid in macrophages.

1985 ◽  
Vol 100 (3) ◽  
pp. 851-859 ◽  
Author(s):  
J A Swanson ◽  
B D Yirinec ◽  
S C Silverstein
Keyword(s):  
Steady State ◽  
Horseradish Peroxidase ◽  
Phorbol Esters ◽  
Lucifer Yellow ◽  
Fluid Phase ◽  
Peritoneal Macrophages ◽  
Tumor Promoter ◽  
Steady State Rate ◽  
Reduced Rate ◽  
Rate Of Accumulation

Lucifer Yellow CH (LY) is an excellent probe for fluid-phase pinocytosis. It accumulates within the macrophage vacuolar system, is not degraded, and is not toxic at concentrations of 6.0 mg/ml. Its uptake is inhibited at 0 degree C. Thioglycollate-elicited mouse peritoneal macrophages were found to exhibit curvilinear uptake kinetics of LY. Upon addition of LY to the medium, there was a brief period of very rapid cellular accumulation of the dye (1,400 ng of LY/mg protein per h at 1 mg/ml LY). This rate of accumulation most closely approximates the rate of fluid influx by pinocytosis. Within 60 min, the rate of LY accumulation slowed to a steady-state rate of 250 ng/mg protein per h which then continued for up to 18 h. Pulse-chase experiments revealed that the reduced rate of accumulation under steady-state conditions was due to efflux of LY. Only 20% of LY taken into the cells was retained; the remainder was released back into the medium. Efflux has two components, rapid and slow; each can be characterized kinetically as a first-order reaction. The kinetics are similar to those described by Besterman et al. (Besterman, J. M., J. A. Airhart, R. C. Woodworth, and R. B. Low, 1981, J. Cell Biol. 91:716-727) who interpret fluid-phase pinocytosis as involving at least two compartments, one small, rapidly turning over compartment and another apparently larger one which fills and empties slowly. To search for processes that control intracellular fluid traffic, we studied pinocytosis after treatment of macrophages with horseradish peroxidase (HRP) or with the tumor promoter phorbol myristate acetate (PMA). HRP, often used as a marker for fluid-phase pinocytosis, was observed to stimulate the rate of LY accumulation in macrophages. PMA caused an immediate four- to sevenfold increase in the rate of LY accumulation. Both HRP and PMA increased LY accumulation by stimulating influx and reducing the percentage of internalized fluid that is rapidly recycled. A greater proportion of endocytosed fluid passes into the slowly emptying compartment (presumed lysosomes). These experiments demonstrate that because of the considerable efflux by cells, measurement of marker accumulation inaccurately estimates the rate of fluid pinocytosis. Moreover, pinocytic flow of water and solutes through cytoplasm is subject to regulation at points beyond the formation of pinosomes.

scholarly journals Immunocytochemical identification of dye-injected cells.

1982 ◽  
Vol 30 (2) ◽  
pp. 189-191 ◽  
Author(s):  
R L Michaels
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Lucifer Yellow ◽  
Immunofluorescent Staining ◽  
Pancreatic Islet Cells ◽  
Lucifer Yellow Ch ◽  
Before And After ◽  
Coupled Cells ◽  
Indirect Immunofluorescent

Lucifer Yellow CH may be injected into pancreatic islet cells and visualized in Epon sections of the embedded tissue both before and after plastic removal and immunocyto-chemical staining. The dye retains its fluorescence, clearly marking the injected cell and adjacent dye-coupled cells, but does not interfere with the indirect immunofluorescent staining patterns that are characteristic of the islet cells

scholarly journals Ganglionic and non-ganglionic neurons in frog heart: do they differ?

2017 ◽  
Vol 26 (2) ◽  
pp. 9
Author(s):  
Darius Batulevičius ◽  
Gertrūda Skripkienė ◽  
Greta Graužinytė ◽  
Augustina Grigaitė ◽  
Valdas Skripka
Keyword(s):  
Rana Temporaria ◽  
Lucifer Yellow ◽  
Frog Heart ◽  
Total Length ◽  
Fluorescent Markers ◽  
Lucifer Yellow Ch ◽  
Frog Rana Temporaria ◽  
The Mean ◽  
Morphological Differences ◽  
Intracardiac Neurons

This study was designed to compare the morphology of neurons in relation to their distance from the major nerve trunks in the heart of the frog Rana temporaria. Seventy-nine intracardiac neurons were labelled intracellularly with fluorescent markers Lucifer Yellow CH and Alexa Fluor 568. The neurons located on the extensions of the vagus nerve were considered as ganglionic, while neurons spread loosely at further distance from these extensions were considered as non-ganglionic. The mean area of the soma in ganglionic neurons was about 25% larger than in non-ganglionic neurons. Ganglionic neurons had a higher soma area/nucleus area ratio than non-ganglionic neurons. Although both the total number and the total length of dendrite-like processes was similar between the two groups, ganglionic neurons had significantly fewer dendrite-like processes from the soma (1.5±0.3 vs. 3.9±1.0; P<0.05) and shorter total length of these processes from the soma (63±18 μm vs. 178±51 μm; P<0.05). In conclusion, ganglionic and non-ganglionic frog intracardiac neurons exhibit substantial morphological differences. We hypothesize that these differences may indicate different projections or variations in the number of their preganglionic inputs.

Curcumin does not alter the phorbol ester effect on cell—cell transfer of Lucifer Yellow CH

1995 ◽  
Vol 16 (5) ◽  
pp. 1229-1231 ◽  
Author(s):  
G. Pásti ◽  
P. Kertai ◽  
R. Ádány
Keyword(s):  
Phorbol Ester ◽  
Lucifer Yellow ◽  
Cell Transfer ◽  
Lucifer Yellow Ch ◽  
Cell Cell

Mapping of neuronal contacts with intracellular injection of horseradish peroxidase and Lucifer yellow in combination

1981 ◽  
Vol 217 (1) ◽  
pp. 143-149 ◽  
Author(s):  
E.R. Macagno ◽  
K.J. Muller ◽  
W.B. Kristan ◽  
S.A. Deriemer ◽  
R. Stewart ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Lucifer Yellow ◽  
Intracellular Injection

Electrical potentials and cell-to-cell dye movement in mouse mammary gland during lactation

1984 ◽  
Vol 247 (1) ◽  
pp. C20-C25 ◽  
Author(s):  
S. E. Berga
Keyword(s):  
Mammary Gland ◽  
Lucifer Yellow ◽  
Mammary Epithelium ◽  
Mouse Mammary Gland ◽  
Dye Transfer ◽  
Alveolar Cells ◽  
Electrical Potentials ◽  
Lucifer Yellow Ch ◽  
Electrophysiological Studies

Stable potentials were recorded with microelectrodes in an in vivo preparation of the mammary gland from the anesthetized lactating mouse. Location of the microelectrode tip was determined by ionophoretic injection of the fluorescent dye Lucifer yellow CH. Fifteen dye injections were localized to mammary alveolar cells; the average recorded potential for these penetrations was -49 +/- 2 mV. Cell-to-cell dye transfer between alveolar cells was observed with all intracellular Lucifer yellow injections. Ten dye injections were localized to the alveolar lumina with an average recorded potential of -35 +/- 2 mV. With these penetrations Lucifer yellow spread rapidly to many alveolar lumina. These findings indicate that stable potentials can be obtained from both cells and lumina in the in vivo mammary gland, demonstrating the feasibility of electrophysiological studies of the mammary epithelium. The presence of a large transepithelial potential provides evidence for physiologically tight junctions between mammary alveolar cells. In addition, the distribution of Lucifer yellow shows that mammary alveolar cells are coupled and suggests that milk flows freely between alveolar lumina.

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