Electron microscopic studies of the dentate gyrus of the rat. I. Normal structure with special reference to synaptic organization

1966 ◽  
Vol 128 (3) ◽  
pp. 359-395 ◽  
Author(s):  
R. H. Laatsch ◽  
W. M. Cowan
Author(s):  
S. K. MAJUMDAR ◽  
FRED KALENSCHER

Ultrathin sections made from bilaterally vasectomized as well as bilaterally sham-operated Mongolian gerbils (Meriones unguiculatus) were examined and compared under an electron microscope in order to determine whether vasectomy has any effect upon the fine structure of the testis. The whole testes were removed and placed in Karnovsky's fixative for one hour. After this period the testes were diced into small pieces and fixed for an additional hour in the same fixative. After rinsing in distilled water and postfixed for one hour in OsO4, the tissues were embedded in Epon 812. The sections were stained with uranyl acetate-lead citrate and examined on a Philips Model 201 transmission electron microscope. Shamoperated testis exhibited normal structure of germ cells.


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