The fine structure of sympathetic neurons in man

1964 ◽  
Vol 122 (1) ◽  
pp. 19-67 ◽  
Author(s):  
Joseph Pick ◽  
Carmen De Lemos ◽  
Carin Gerdin
Keyword(s):  
Fine Structure ◽  
Sympathetic Neurons

scholarly journals THE FINE STRUCTURE OF SYMPATHETIC NEURONS IN X-IRRADIATED FROGS

1965 ◽  
Vol 26 (2) ◽  
pp. 335-351 ◽  
Author(s):  
Joseph Pick
Keyword(s):  
Fine Structure ◽  
Sympathetic Neurons ◽  
Sympathetic Ganglia ◽  
Nerve Fibers ◽  
Whole Body ◽  
Complete Fusion ◽  
Unmyelinated Fibers ◽  
Standard Procedures ◽  
Myelinated And Unmyelinated Fibers ◽  
X Irradiation

The effects of whole body x-irradiation on the fine structure of sympathetic neurons were studied in 15 unanesthetized adult frogs (Rana pipiens), as seen at intervals ranging from 1 hour to 2 weeks after single exposures to 1000 r and 2000 r. Using standard procedures, the lumbar sympathetic ganglia of experimental and 20 control animals were prepared for electron microscope examination. Radiation produced conspicuous but irregular and variable deterioration, swelling, and clearing of neuronal lysosomes. These changes may have been due to an increased permeability of lysosomal membranes, causing the entry of fluid into lysosomes and their swelling and deterioration, but a pronounced escape of lysosomal enzymes into the cytoplasm was questionable. Less frequent were the dilatation and the parallel layering or complete fusion and tight packing of the rough-edged endoplasmic reticulum. The number of vacuoles, probably derived from Golgi cisternae, was somewhat increased. These vacuoles were conjectured to serve the "sequestration" of damaged cytoplasmic areas. Abnormal amounts of presumptive glycogen granules occupied some axons of myelinated and unmyelinated fibers, especially of presynaptic nerve fibers. This was assumed to be due to a decreased breakdown of glycogen and probably caused the interruption of the transmission of nerve impulses in presynaptic fibers. The maximal incidence of these alterations seemingly occurred 8 days after exposure to 1000 r, and 1 hour after x-irradiation with 2000 r. Signs of recovery appeared 2 weeks after exposure to 2000 r.

The fine structure of thoracic sympathetic neurons in the adult rat

1966 ◽  
Vol 71 (2) ◽  
pp. 189-206 ◽  
Author(s):  
Carmen Lemos ◽  
Joseph Pick
Keyword(s):  
Fine Structure ◽  
Sympathetic Neurons ◽  
Adult Rat

scholarly journals FINE STRUCTURE OF NERVE FIBERS AND GROWTH CONES OF ISOLATED SYMPATHETIC NEURONS IN CULTURE

1973 ◽  
Vol 56 (3) ◽  
pp. 713-735 ◽  
Author(s):  
Mary Bartlett Bunge
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Endoplasmic Reticulum ◽  
Fine Structure ◽  
Sensory Neuron ◽  
Sympathetic Neurons ◽  
Growth Cones ◽  
Nerve Fibers ◽  
Cervical Ganglia ◽  
Neuron Growth

The leading tips of elongating nerve fibers are enlarged into "growth cones" which are seen in tissue culture to continually undergo changes in conformation and to foster numerous transitory slender extensions (filopodia) and/or a veillike ruffling sheet. After explantation of 1-day-old rat superior cervical ganglia (as pieces or as individual neurons), nerve fibers and tips were photographed during growth and through the initial stages of aldehyde fixation and then relocated after embedding in plastic. Electron microscopy of serially sectioned tips revealed the following. The moving parts of the cone, the peripheral flange and filopodia, contained a distinctive apparently filamentous feltwork from which all organelles except membranous structures were excluded; microtubules were notably absent from these areas. The cone interior contained varied forms of agranular endoplasmic reticulum, vacuoles, vesicles, coated vesicles, mitochondria, microtubules, and occasional neurofilaments and polysomes. Dense-cored vesicles and lysosomal structures were also present and appeared to be formed locally, at least in part from reticulum. The possible roles of the various forms of agranular membranous components are discussed and it is suggested that structures involved in both the assembly and degradation of membrane are present in the cone. The content of these growing tips resembles that in sensory neuron growth cones studied by others.

Alterations in the fine structure of nucleoli in sympathetic neurons following NGF-antiserum treatment

1969 ◽  
Vol 12 (1) ◽  
pp. 54-73 ◽  
Author(s):  
Rita Levi-Montalcini ◽  
Felice Caramia ◽  
Pietro U. Angeletti
Keyword(s):  
Fine Structure ◽  
Sympathetic Neurons

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

Pituitary Follicular Cells: Their Origin, Possible Function and Fate

1974 ◽  
Vol 32 ◽  
pp. 34-35
Author(s):  
E. Horvath ◽  
K. Kovacs ◽  
G. Penz ◽  
C. Ezrin
Keyword(s):  
Fine Structure ◽  
Secretory Granules ◽  
Follicular Cells ◽  
Human Pituitary ◽  
Rat Pituitary ◽  
Pituitary Glands ◽  
Junctional Complexes

Follicular structures, in the rat pituitary, composed of cells joined by junctional complexes and possessing few organelles and few, if any, secretory granules, were first described by Farquhar in 1957. Cells of the same description have since been observed in several species including man. The importance of these cells, however, remains obscure. While studying human pituitary glands, we have observed wide variations in the fine structure of follicular cells which may lead to a better understanding of their morphogenesis and significance.

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

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