scholarly journals Region‐ and neuronal‐subtype‐specific expression of Na, K‐ATPase alpha and beta subunit isoforms in the mouse brain

2020 ◽  
Vol 528 (16) ◽  
pp. 2654-2678 ◽  
Author(s):  
Koshi Murata ◽  
Tomoki Kinoshita ◽  
Tatsuya Ishikawa ◽  
Kazuki Kuroda ◽  
Minako Hoshi ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Beta Subunit ◽  
Specific Expression ◽  
Neuronal Subtype ◽  
Subunit Isoforms

scholarly journals A cell type‐specific expression map of NCoR1 and SMRT transcriptional co‐repressors in the mouse brain

2020 ◽  
Vol 528 (13) ◽  
pp. 2218-2238 ◽  
Author(s):  
Attilio Iemolo ◽  
Patricia Montilla‐Perez ◽  
I‐Chi Lai ◽  
Yinuo Meng ◽  
Syreeta Nolan ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Cell Type ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
A Cell ◽  
Cell Type Specific

A spectrin-like protein from mouse brain membranes: phosphorylation of the 235,000-dalton subunit

1984 ◽  
Vol 247 (1) ◽  
pp. C61-C73 ◽  
Author(s):  
S. R. Goodman ◽  
I. S. Zagon ◽  
C. F. Whitfield ◽  
L. A. Casoria ◽  
S. B. Shohet ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Beta Subunit ◽  
Beta Subunits ◽  
Mouse Erythrocyte ◽  
Alpha Beta ◽  
Beta 2 ◽  
Independent Protein ◽  
Brain Spectrin

A mouse brain spectrin-like protein, which was an immunoreactive analogue of erythrocyte spectrin, has been isolated from demyelinated membranes. This spectrin analogue was a 10.5 S, 972,000 molecular weight (Mr) (alpha beta)2 tetramer containing subunits of 240,000 (alpha) and 235,000 (beta) Mr. We demonstrated that in vivo only the 235,000 Mr beta subunit of the mouse brain spectrin-like protein was phosphorylated, which was an analogous situation to mouse erythrocyte spectrin in which only the 220,000 Mr beta subunit was phosphorylated. Incubation of isolated membrane fractions with [gamma-32P]ATP +/- adenosine 3',5'-cyclic monophosphate (cAMP) indicated that mouse brain spectrin-like protein, mouse erythrocyte spectrin, and human erythrocyte spectrin's beta subunits were all phosphorylated in vitro by membrane-associated cAMP-independent protein kinases.

scholarly journals Cell-specific expression of high levels of human S100 beta in transgenic mouse brain is dependent on gene dosage

1992 ◽  
Vol 12 (11) ◽  
pp. 4337-4346 ◽  
Author(s):  
WC Friend ◽  
S Clapoff ◽  
C Landry ◽  
LE Becker ◽  
D O'Hanlon ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Brain ◽  
Gene Dosage ◽  
Specific Expression

scholarly journals AMPK and the Need to Breathe and Feed: What’s the Matter with Oxygen?

2020 ◽  
Vol 21 (10) ◽  
pp. 3518 ◽  
Author(s):  
A. Mark Evans ◽  
D. Grahame Hardie
Keyword(s):  
Food Choice ◽  
Oxygen Supply ◽  
The Body ◽  
Specific Expression ◽  
Subunit Isoforms ◽  
Specific Expression Pattern ◽  
Available Information ◽  
Amp Activated Protein Kinase ◽  
Do So

We live and to do so we must breathe and eat, so are we a combination of what we eat and breathe? Here, we will consider this question, and the role in this respect of the AMP-activated protein kinase (AMPK). Emerging evidence suggests that AMPK facilitates central and peripheral reflexes that coordinate breathing and oxygen supply, and contributes to the central regulation of feeding and food choice. We propose, therefore, that oxygen supply to the body is aligned with not only the quantity we eat, but also nutrient-based diet selection, and that the cell-specific expression pattern of AMPK subunit isoforms is critical to appropriate system alignment in this respect. Currently available information on how oxygen supply may be aligned with feeding and food choice, or vice versa, through our motivation to breathe and select particular nutrients is sparse, fragmented and lacks any integrated understanding. By addressing this, we aim to provide the foundations for a clinical perspective that reveals untapped potential, by highlighting how aberrant cell-specific changes in the expression of AMPK subunit isoforms could give rise, in part, to known associations between metabolic disease, such as obesity and type 2 diabetes, sleep-disordered breathing, pulmonary hypertension and acute respiratory distress syndrome.

Analysis of subunit assembly of the Na-K-ATPase

1994 ◽  
Vol 266 (3) ◽  
pp. C579-C589 ◽  
Author(s):  
D. M. Fambrough ◽  
M. V. Lemas ◽  
M. Hamrick ◽  
M. Emerick ◽  
K. J. Renaud ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Sodium Pump ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Beta Subunits ◽  
Limited Region ◽  
Subunit Assembly ◽  
Alpha Subunits ◽  
Subunit Isoforms ◽  
P Type

The Na-K-ATPase, or sodium pump, is comprised of two subunits, alpha and beta. Each subunit spans the lipid bilayer of the cell membrane. This review summarizes our efforts to determine how the two subunits interact to form the functional ion transporter. Our major approach has been to observe the potential for subunit assembly when one or both subunits are truncated or present as chimeras that retain only a limited region of the Na-K-ATPase. DNAs encoding these altered subunit forms of the avian Na-K-ATPase are expressed in mammalian cells. Monoclonal antibodies specific for the avian beta-subunit are then used to purify newly synthesized avian beta-subunits, and the presence of accompanying alpha-subunits indicates that subunit assembly has occurred. The ectodomain of the beta-subunit (approximately residues 62-304) is sufficient for assembly with the alpha-subunit, and a COOH-terminal truncation of the beta-subunit that lacks aminoacyl residues beyond 162 will assemble inefficiently. A maximum of 26 aminoacyl residues of the alpha-subunit are necessary for robust assembly with the beta-subunit, when this sequence replaces the COOH-terminal half of the loop between membrane spans 7 and 8 in the SERCA1 Ca-ATPase. This region of the Ca-ATPase faces the lumen of the endoplasmic reticulum. These findings encourage study of other related questions, including whether there is preferential assembly of certain subunit isoforms and how various P-type ATPases are targeted to their appropriate subcellular compartments.

A versatile system for the neuronal subtype specific expression of lentiviral vectors

2009 ◽  
Vol 24 (3) ◽  
pp. 723-730 ◽  
Author(s):  
Stefania Tolu ◽  
M. Elena Avale ◽  
Hiroko Nakatani ◽  
Stephanie Pons ◽  
Sebastien Parnaudeau ◽  
...  
Keyword(s):  
Lentiviral Vectors ◽  
Specific Expression ◽  
Neuronal Subtype
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