scholarly journals Characterization of Drosophila octopamine receptor neuronal expression using MiMIC‐converted Gal4 lines

2020 ◽  
Vol 528 (13) ◽  
pp. 2174-2194
Author(s):  
Hannah M. McKinney ◽  
Lewis M. Sherer ◽  
Jessica L. Williams ◽  
Sarah J. Certel ◽  
R. Steven Stowers
Keyword(s):  
Octopamine Receptor ◽  
Neuronal Expression

Author response for "Characterization of Drosophila Octopamine Receptor Neuronal Expression using MiMIC‐converted Gal4 lines"

2020 ◽  
Author(s):  
Hannah McKinney ◽  
Lewis M. Sherer ◽  
Jessica L. Williams ◽  
Sarah J. Certel ◽  
R. Steven Stowers
Keyword(s):  
Author Response ◽  
Octopamine Receptor ◽  
Neuronal Expression

The characterization of presynaptic octopamine receptors modulating octopamine release from an identified neurone in the locust

1998 ◽  
Vol 201 (13) ◽  
pp. 2053-2060 ◽  
Author(s):  
KM Howell ◽  
PD Evans
Keyword(s):  
Central Nervous System ◽  
Rank Order ◽  
Muscle Preparation ◽  
Pharmacological Profile ◽  
Octopamine Receptors ◽  
High K ◽  
Wide Range ◽  
Octopamine Receptor ◽  
Dorsal Unpaired Median

Octopamine release has been demonstrated from the dorsal unpaired median neurone to the locust extensor-tibiae muscle (DUMETi) in response to high-[K+] saline. Here, we provide evidence for the existence of presynaptic inhibitory autoreceptors for octopamine on the DUMETi terminals and report on their pharmacological profile. Octopamine release was initiated by exposure to high-[K+] saline (0. 1 mol l-1) and measured using a radioenzyme assay for octopamine. Octopamine receptor antagonists (10(-4 )mol l-1) potentiated the high-[K+]-mediated release of octopamine with the following rank order of potency: phentolamine = metoclopramide > mianserin = chlorpromazine > cyproheptadine > yohimbine. Octopamine receptor agonists (10(-4 )mol l-1) inhibited the high-[K+]-mediated release of octopamine with the following rank order of potency: naphazoline > tolazoline > clonidine. Thus, the octopamine autoreceptors on the DUMETi terminals are much closer pharmacologically to the pre-and postsynaptic OCTOPAMINE2 receptors in the locust extensor-tibiae muscle preparation than to the OCTOPAMINE3 receptors from the locust central nervous system. The results suggest that there is likely to be more than one type of insect neuronal octopamine receptor. It is also likely that presynaptic modulation of octopamine release may be confined to octopamine receptors since a wide range of other putative modulatory substances did not produce this effect.

scholarly journals AmOctα2R: Functional Characterization of a Honeybee Octopamine Receptor Inhibiting Adenylyl Cyclase Activity

2020 ◽  
Vol 21 (24) ◽  
pp. 9334
Author(s):  
Wolfgang Blenau ◽  
Joana Alessandra Wilms ◽  
Sabine Balfanz ◽  
Arnd Baumann
Keyword(s):  
Biogenic Amines ◽  
Functional Characterization ◽  
Eukaryotic Cell ◽  
Intracellular Camp ◽  
Octopamine Receptors ◽  
Pharmacological Data ◽  
Large Protein Family ◽  
Octopamine Receptor ◽  
G Protein Coupled

The catecholamines norepinephrine and epinephrine are important regulators of vertebrate physiology. Insects such as honeybees do not synthesize these neuroactive substances. Instead, they use the phenolamines tyramine and octopamine for similar physiological functions. These biogenic amines activate specific members of the large protein family of G protein-coupled receptors (GPCRs). Based on molecular and pharmacological data, insect octopamine receptors were classified as either α- or β-adrenergic-like octopamine receptors. Currently, one α- and four β-receptors have been molecularly and pharmacologically characterized in the honeybee. Recently, an α2-adrenergic-like octopamine receptor was identified in Drosophila melanogaster (DmOctα2R). This receptor is activated by octopamine and other biogenic amines and causes a decrease in intracellular cAMP ([cAMP]i). Here, we show that the orthologous receptor of the honeybee (AmOctα2R), phylogenetically groups in a clade closely related to human α2-adrenergic receptors. When heterologously expressed in an eukaryotic cell line, AmOctα2R causes a decrease in [cAMP]i. The receptor displays a pronounced preference for octopamine over tyramine. In contrast to DmOctα2R, the honeybee receptor is not activated by serotonin. Its activity can be blocked efficiently by 5-carboxamidotryptamine and phentolamine. The functional characterization of AmOctα2R now adds a sixth member to this subfamily of monoaminergic receptors in the honeybee and is an important step towards understanding the actions of octopamine in honeybee behavior and physiology.

scholarly journals Molecular and functional characterization of an octopamine receptor from honeybee (Apis mellifera) brain

2003 ◽  
Vol 86 (3) ◽  
pp. 725-735 ◽  
Author(s):  
Lore Grohmann ◽  
Wolfgang Blenau ◽  
Joachim Erber ◽  
Paul R. Ebert ◽  
Timo Strünker ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Functional Characterization ◽  
Octopamine Receptor
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