scholarly journals Distributions of hypothalamic neuron populations coexpressing tyrosine hydroxylase and the vesicular GABA transporter in the mouse

2020 ◽  
Vol 528 (11) ◽  
pp. 1833-1855 ◽  
Author(s):  
Kenichiro Negishi ◽  
Mikayla A. Payant ◽  
Kayla S. Schumacker ◽  
Gabor Wittmann ◽  
Rebecca M. Butler ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Hypothalamic Neuron ◽  
Gaba Transporter ◽  
Neuron Populations

scholarly journals Distributions of hypothalamic neuron populations co-expressing tyrosine hydroxylase and the vesicular GABA transporter in the mouse

2019 ◽  
Author(s):  
Kenichiro Negishi ◽  
Mikayla A. Payant ◽  
Kayla S. Schumacker ◽  
Gabor Wittmann ◽  
Rebecca M. Butler ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Brain Regions ◽  
Zona Incerta ◽  
Gaba Transporter ◽  
Hypothalamic Region ◽  
Neuron Populations ◽  
Gfp Fluorescence ◽  
Multiple Chemical

AbstractThe hypothalamus contains catecholaminergic neurons marked by the expression of tyrosine hydroxylase (TH). As multiple chemical messengers coexist in each neuron, we determined if hypothalamic TH-immunoreactive (ir) neurons express glutamate or GABA. We used Cre/loxP recombination to express enhanced GFP fluorescence (EGFP) in neurons that express the vesicular glutamate (vGLUT2) or GABA transporter (vGAT), then determined TH immunoreactivity in glutamatergic or GABAergic neurons, respectively. EGFP-positive vGLUT2 neurons were not TH-ir. However, discrete TH-ir signals colocalized with EGFP-positive vGAT neurons, which we validated by in situ hybridization for Vgat mRNA. In order to contextualize the observed pattern of TH+EGFP colocalization in vGAT neurons, we first performed Nissl-based parcellation and plane-of-section analysis, and then mapped the distribution of TH-ir vGAT neurons onto atlas templates from the Allen Reference Atlas (ARA) of the mouse brain. TH-ir vGAT neurons were distributed throughout the rostrocaudal extent of the hypothalamus. Within the ARA ontology of gray matter regions, TH-ir neurons localized primarily to the periventricular hypothalamic zone, periventricular hypothalamic region, and lateral hypothalamic zone. There was a very strong presence of EGFP fluorescence in TH-ir neurons across all brain regions, but the most striking colocalization was found in the zona incerta (ZI) – a region assigned to the hypothalamus in the ARA – where every TH-ir neuron was EGFP-positive. Neurochemical characterization of these ZI neurons revealed that they display immunoreactivity for dopamine but not dopamine β-hydroxylase. In aggregate, these findings indicate the existence of a novel hypothalamic population in the mouse that may signal through the release of GABA and/or dopamine.

Exercise Increases NPY/AgRP and TH Neuron Activity in the Hypothalamus of Female Mice

2021 ◽  
Author(s):  
Taylor Landry ◽  
Daniel Shookster ◽  
Alec Chaves ◽  
Katrina Free ◽  
Tony Nguyen ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Food Intake ◽  
Aerobic Exercise ◽  
High Intensity ◽  
Treadmill Exercise ◽  
High Intensity Exercise ◽  
Neuron Activity ◽  
Post Exercise ◽  
Female Mice ◽  
Neuron Populations

Recent evidence identifies a potent role for aerobic exercise to modulate activity of hypothalamic neurons related to appetite; however, these studies have been primarily performed in male rodents. Since females have markedly different neuronal mechanisms regulating food intake, the current study aimed to determine the effects of acute treadmill exercise on hypothalamic neuron populations involved in regulating appetite in female mice. Mature, untrained female mice were exposed to acute sedentary, low (10m/min), moderate (14m/min), and high (18m/min) intensity treadmill exercise in a randomized crossover design. Mice were fasted 10-hours before exercise and food intake was monitored for 48-hours after bouts. Immunohistochemical detection of cFOS was performed 3-hours post-exercise to determine changes in hypothalamic NPY/AgRP, POMC, tyrosine hydroxylase, and SIM1-expressing neuron activity concurrent with changes in food intake. Additionally, stains for pSTAT3tyr705 and pERKthr202/tyr204 were performed to detect exercise-mediated changes in intracellular signaling. Briefly, moderate and high intensity exercise increased 24-hour food intake by 5.9% and 19%, respectively, while low intensity exercise had no effects. Furthermore, increases in NPY/AgRPARC, SIM1PVN, and tyrosine hydroxylase neuron activity were observed 3-hours after high intensity exercise, with no effects on POMCARC neurons. While no effects of exercise on pERKthr202/tyr204 were observed, pSTAT3tyr705 was elevated specifically in NPY/AgRP neurons 3-hours post-exercise. Overall, aerobic exercise increased activity of several appetite-stimulating neuron populations in the hypothalamus of female mice, which may provide insight into previously reported sexual dimorphisms in post-exercise feeding.

Colocalization of tyrosine hydroxylase and GAD65 mRNA in mesostriatal neurons

2001 ◽  
Vol 13 (1) ◽  
pp. 57-67 ◽  
Author(s):  
Tomas Gonzalez-Hernandez ◽  
Pedro Barroso-Chinea ◽  
Abraham Acevedo ◽  
Eduardo Salido ◽  
Manuel Rodriguez
Keyword(s):  
Tyrosine Hydroxylase

Diurnal Rhythms of Tyrosine Hydroxylase Expression are Regulated by NAD Cellular Redox and SIRT1

2018 ◽  
Author(s):  
Ryan W. Logan ◽  
Puja K. Parekh ◽  
Wilbur Williams III ◽  
Gabrielle Kaplan ◽  
Darius Becker-Krail ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Diurnal Rhythms ◽  
Cellular Redox

A Novel Prion Protein-Tyrosine Hydroxylase Interaction

2014 ◽  
Vol 13 (5) ◽  
pp. 896-908 ◽  
Author(s):  
Mattia Vicario ◽  
Adriana Zagari ◽  
Vincenzo Granata ◽  
Francesca Munari ◽  
Stefano Mammi ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Prion Protein ◽  
Protein Tyrosine
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