Involvement of sonic hedgehog and notch signaling in regenerative neurogenesis in adult zebrafish optic tectum after stab injury

2018 ◽  
Vol 526 (15) ◽  
pp. 2360-2372 ◽  
Author(s):  
Yuto Ueda ◽  
Yuki Shimizu ◽  
Nobuyuki Shimizu ◽  
Tohru Ishitani ◽  
Toshio Ohshima
Keyword(s):  
Notch Signaling ◽  
Sonic Hedgehog ◽  
Optic Tectum ◽  
Adult Zebrafish ◽  
Stab Injury ◽  
Zebrafish Optic Tectum

scholarly journals Valproic acid, a histone deacetylase inhibitor, regulates cell proliferation in the adult zebrafish optic tectum

2014 ◽  
Vol 243 (11) ◽  
pp. 1401-1415 ◽  
Author(s):  
Miki Dozawa ◽  
Hiromitsu Kono ◽  
Yuki Sato ◽  
Yoko Ito ◽  
Hideomi Tanaka ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Cell Proliferation ◽  
Histone Deacetylase ◽  
Optic Tectum ◽  
Histone Deacetylase Inhibitor ◽  
Adult Zebrafish ◽  
Deacetylase Inhibitor ◽  
Zebrafish Optic Tectum

scholarly journals Characterization of neural stem cells and their progeny in the adult zebrafish optic tectum

2010 ◽  
Vol 342 (1) ◽  
pp. 26-38 ◽  
Author(s):  
Yoko Ito ◽  
Hideomi Tanaka ◽  
Hitoshi Okamoto ◽  
Toshio Ohshima
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Optic Tectum ◽  
Adult Zebrafish ◽  
Zebrafish Optic Tectum

scholarly journals Transcriptome Analyses Reveal IL6/Stat3 Signaling Involvement in Radial Glia Proliferation After Stab Wound Injury in the Adult Zebrafish Optic Tectum

2021 ◽  
Vol 9 ◽  
Author(s):  
Yuki Shimizu ◽  
Mariko Kiyooka ◽  
Toshio Ohshima
Keyword(s):  
Optic Tectum ◽  
Cellular Response ◽  
Molecular Mechanisms ◽  
Radial Glia ◽  
Stab Wound ◽  
Cytokine Signaling ◽  
Adult Zebrafish ◽  
Stat3 Signaling ◽  
Proliferation And Differentiation ◽  
Zebrafish Optic Tectum

Adult zebrafish have many neurogenic niches and a high capacity for central nervous system regeneration compared to mammals, including humans and rodents. The majority of radial glia (RG) in the zebrafish optic tectum are quiescent under physiological conditions; however, stab wound injury induces their proliferation and differentiation into newborn neurons. Although previous studies have functionally analyzed the molecular mechanisms of RG proliferation and differentiation and have performed single-cell transcriptomic analyses around the peak of RG proliferation, the cellular response and changes in global gene expression during the early stages of tectum regeneration remain poorly understood. In this study, we performed histological analyses which revealed an increase in isolectin B4+ macrophages prior to the induction of RG proliferation. Moreover, transcriptome and pathway analyses based on differentially expressed genes identified various enriched pathways, including apoptosis, the innate immune system, cell proliferation, cytokine signaling, p53 signaling, and IL6/Jak-Stat signaling. In particular, we found that Stat3 inhibition suppressed RG proliferation after stab wound injury and that IL6 administration into cerebroventricular fluid activates RG proliferation without causing injury. Together, the findings of these transcriptomic and functional analyses reveal that IL6/Stat3 signaling is an initial trigger of RG activation during optic tectum regeneration.

scholarly journals Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum

2011 ◽  
Vol 75 (3) ◽  
pp. 325-333 ◽  
Author(s):  
Christopher P. Corbo ◽  
Nidaa A. Othman ◽  
Michael C. Gutkin ◽  
Alejandra Del C. Alonso ◽  
Zoltan L. Fulop
Keyword(s):  
Optic Tectum ◽  
Cellular Composition ◽  
Adult Zebrafish ◽  
Zebrafish Optic Tectum

scholarly journals Critical Effects on Akt Signaling in Adult Zebrafish Brain Following Alterations in Light Exposure

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 637
Author(s):  
Nicholas S. Moore ◽  
Robert A. Mans ◽  
Mackenzee K. McCauley ◽  
Colton S. Allgood ◽  
Keri A. Barksdale
Keyword(s):  
Optic Tectum ◽  
Visual Processing ◽  
Environmental Enrichment ◽  
Glycogen Synthase ◽  
Light Exposure ◽  
Hsp70 Expression ◽  
Light Cycle ◽  
Adult Zebrafish ◽  
Sleep State ◽  
Zebrafish Brain

Evidence from human and animal studies indicate that disrupted light cycles leads to alterations of the sleep state, poor cognition, and the risk of developing neuroinflammatory and generalized health disorders. Zebrafish exhibit a diurnal circadian rhythm and are an increasingly popular model in studies of neurophysiology and neuropathophysiology. Here, we investigate the effect of alterations in light cycle on the adult zebrafish brain: we measured the effect of altered, unpredictable light exposure in adult zebrafish telencephalon, homologous to mammalian hippocampus, and the optic tectum, a significant visual processing center with extensive telencephalon connections. The expression of heat shock protein-70 (HSP70), an important cell stress mediator, was significantly decreased in optic tectum of adult zebrafish brain following four days of altered light exposure. Further, pSer473-Akt (protein kinase B) was significantly reduced in telencephalon following light cycle alteration, and pSer9-GSK3β (glycogen synthase kinase-3β) was significantly reduced in both the telencephalon and optic tectum of light-altered fish. Animals exposed to five minutes of environmental enrichment showed significant increase in pSer473Akt, which was significantly attenuated by four days of altered light exposure. These data show for the first time that unpredictable light exposure alters HSP70 expression and dysregulates Akt-GSK3β signaling in the adult zebrafish brain.

Sonic Hedgehog Regulation of the Neural Precursor Cell Fate During Chicken Optic Tectum Development

2017 ◽  
Vol 64 (2) ◽  
pp. 287-299 ◽  
Author(s):  
Ciqing Yang ◽  
Xiaoying Li ◽  
Qiuling Li ◽  
Han Li ◽  
Liang Qiao ◽  
...  
Keyword(s):  
Cell Fate ◽  
Sonic Hedgehog ◽  
Optic Tectum ◽  
Precursor Cell ◽  
Neural Precursor ◽  
Neural Precursor Cell

scholarly journals Adaptation-induced modification of motion selectivity tuning in visual tectal neurons of adult zebrafish

2015 ◽  
Vol 114 (5) ◽  
pp. 2893-2902 ◽  
Author(s):  
Vanessa Hollmann ◽  
Valerie Lucks ◽  
Rafael Kurtz ◽  
Jacob Engelmann
Keyword(s):  
Optic Tectum ◽  
Brain Area ◽  
Direction Selectivity ◽  
Adult Brain ◽  
Orientation Tuning ◽  
Adult Zebrafish ◽  
Short Term ◽  
Short Term Plasticity ◽  
The Difference ◽  
Tectal Neurons

In the developing brain, training-induced emergence of direction selectivity and plasticity of orientation tuning appear to be widespread phenomena. These are found in the visual pathway across different classes of vertebrates. Moreover, short-term plasticity of orientation tuning in the adult brain has been demonstrated in several species of mammals. However, it is unclear whether neuronal orientation and direction selectivity in nonmammalian species remains modifiable through short-term plasticity in the fully developed brain. To address this question, we analyzed motion tuning of neurons in the optic tectum of adult zebrafish by calcium imaging. In total, orientation and direction selectivity was enhanced by adaptation, responses of previously orientation-selective neurons were sharpened, and even adaptation-induced emergence of selectivity in previously nonselective neurons was observed in some cases. The different observed effects are mainly based on the relative distance between the previously preferred and the adaptation direction. In those neurons in which a shift of the preferred orientation or direction was induced by adaptation, repulsive shifts (i.e., away from the adapter) were more prevalent than attractive shifts. A further novel finding for visually induced adaptation that emerged from our study was that repulsive and attractive shifts can occur within one brain area, even with uniform stimuli. The type of shift being induced also depends on the difference between the adapting and the initially preferred stimulus direction. Our data indicate that, even within the fully developed optic tectum, short-term plasticity might have an important role in adjusting neuronal tuning functions to current stimulus conditions.

scholarly journals Induction of Oxidative Stress and Apoptosis in the Injured Brain: Potential Relevance to Brain Regeneration in Zebrafish.

2021 ◽  
Author(s):  
Surendra Kumar Anand ◽  
Manas Ranjan Sahu ◽  
Amal Chandra Mondal
Keyword(s):  
Oxidative Stress ◽  
Mrna Levels ◽  
Adult Zebrafish ◽  
Zebrafish Model ◽  
Stab Injury ◽  
Zebrafish Brain ◽  
Protein Levels ◽  
Injured Brain ◽  
Brain Regeneration ◽  
The Impact

Abstract In the recent years, zebrafish, owing to its tremendous adult neurogenic capacity, has emerged as a useful vertebrate model to study brain regeneration. Recent findings suggest a significant role of the BDNF/TrkB signaling as a mediator of brain regeneration following a stab injury in the adult zebrafish brain. Since BDNF has been implicated in a plethora of physiological processes, we hypothesized that these processes are affected in the injured zebrafish brain. In this small study, we examined the indicators of oxidative stress and of apoptosis using biochemical assays, RT-PCR and IHC to reflect upon the impact of stab injury on oxidative stress levels and apoptosis in the injured adult zebafish brain. Our results indicate induction of oxidative stress in the injured adult zebrafish brain. Also, apoptosis was induced in the injured brain as indicated by increased protein levels of cleaved caspase3 as well as enhanced mRNA levels of both pro-apoptotic and anti-apoptotic genes. This knowledge contributes to the overall understanding of adult neurogenesis in the zebrafish model and raises new questions pertaining to the compensatory physiological mechanisms in response to traumatic brain injury in the adult zebrafish brain.

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