scholarly journals Three-dimensional synaptic analyses of mitral cell and external tufted cell dendrites in rat olfactory bulb glomeruli

2016 ◽  
Vol 525 (3) ◽  
pp. 592-609 ◽  
Author(s):  
Jennifer N. Bourne ◽  
Nathan E. Schoppa
Keyword(s):  
Olfactory Bulb ◽  
Three Dimensional ◽  
Mitral Cell ◽  
Tufted Cell

Parallel Tufted Cell and Mitral Cell Pathways from the Olfactory Bulb to the Olfactory Cortex

2014 ◽  
pp. 133-160 ◽  
Author(s):  
Shin Nagayama ◽  
Kei M. Igarashi ◽  
Hiroyuki Manabe ◽  
Kensaku Mori
Keyword(s):  
Olfactory Bulb ◽  
Mitral Cell ◽  
Olfactory Cortex ◽  
Tufted Cell

scholarly journals Molecular characterization of projection neuron subtypes in the mouse olfactory bulb

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Sara Zeppilli ◽  
Tobias Ackels ◽  
Robin Attey ◽  
Nell Klimpert ◽  
Dr. Kimberly Ritola ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Projection Neuron ◽  
Mitral Cell ◽  
Cell Populations ◽  
Projection Neurons ◽  
Functional Roles ◽  
Entry Points ◽  
Single Nucleus ◽  
Tufted Cell

Projection neurons (PNs) in the mammalian olfactory bulb (OB) receive input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes. Here, we used viral injections into olfactory cortex and fluorescent nucleus sorting to enrich PNs for high-throughput single nucleus and bulk RNA deep sequencing. Transcriptome analysis and RNA in situ hybridization identified distinct mitral and tufted cell populations with characteristic transcription factor network topology, cell adhesion and excitability-related gene expression. Finally, we describe a new computational approach for integrating bulk and snRNA-seq data, and provide evidence that different mitral cell populations preferentially project to different target regions. Together, we have identified potential molecular and gene regulatory mechanisms underlying PN diversity and provide new molecular entry points into studying the diverse functional roles of mitral and tufted cell subtypes.

scholarly journals Molecular characterization of projection neuron subtypes in the mouse olfactory bulb

2020 ◽  
Author(s):  
Sara Zeppilli ◽  
Tobias Ackels ◽  
Robin Attey ◽  
Nell Klimpert ◽  
Kimberly D. Ritola ◽  
...  
Keyword(s):  
Olfactory Bulb ◽  
Projection Neuron ◽  
Mitral Cell ◽  
Olfactory Cortex ◽  
Cell Populations ◽  
Projection Neurons ◽  
Functional Roles ◽  
Entry Points ◽  
Single Nucleus ◽  
Tufted Cell

AbstractProjection neurons (PNs) in the mammalian olfactory bulb (OB) receive direct input from the nose and project to diverse cortical and subcortical areas. Morphological and physiological studies have highlighted functional heterogeneity, yet no molecular markers have been described that delineate PN subtypes. Here, we used viral injections into olfactory cortex and fluorescent nucleus sorting to enrich PNs for high-throughput single nucleus and bulk RNA deep sequencing. Transcriptome analysis and RNA in situ hybridization identified three mitral and five tufted cell populations with characteristic transcription factor network topology and cell adhesion and excitability-related gene expression. Finally, by integrating bulk and snRNA-seq data we propose that different mitral cell populations selectively project to different regions of olfactory cortex. Together, we have identified potential molecular and gene regulatory mechanisms underlying PN diversity and provide new molecular entry points into studying the diverse functional roles of mitral and tufted cell subtypes.

Group I Metabotropic Glutamate Receptors Are Differentially Expressed by Two Populations of Olfactory Bulb Granule Cells

2007 ◽  
Vol 97 (4) ◽  
pp. 3136-3141 ◽  
Author(s):  
Thomas Heinbockel ◽  
Kathryn A. Hamilton ◽  
Matthew Ennis
Keyword(s):  
Olfactory Bulb ◽  
Glutamate Receptors ◽  
Metabotropic Glutamate Receptors ◽  
Granule Cells ◽  
Mitral Cell ◽  
Mitral Cells ◽  
Patch Clamp Recording ◽  
Metabotropic Glutamate ◽  
Group I ◽  
Bath Application

In the main olfactory bulb, several populations of granule cells (GCs) can be distinguished based on the soma location either superficially, interspersed with mitral cells within the mitral cell layer (MCL), or deeper, within the GC layer (GCL). Little is known about the physiological properties of superficial GCs (sGCs) versus deep GCs (dGCs). Here, we used patch-clamp recording methods to explore the role of Group I metabotropic glutamate receptors (mGluRs) in regulating the activity of GCs in slices from wildtype and mGluR−/− mutant mice. In wildtype mice, bath application of the selective Group I mGluR agonist DHPG depolarized and increased the firing rate of both GC subtypes. In the presence of blockers of fast synaptic transmission (APV, CNQX, gabazine), DHPG directly depolarized both GC subtypes, although the two GC subtypes responded differentially to DHPG in mGluR1−/− and mGluR5−/− mice. DHPG depolarized sGCs in slices from mGluR5−/− mice, although it had no effect on sGCs in slices from mGluR1−/− mice. By contrast, DHPG depolarized dGCs in slices from mGluR1−/− mice but had no effect on dGCs in slices from mGluR5−/− mice. Previous studies showed that mitral cells express mGluR1 but not mGluR5. The present results therefore suggest that sGCs are more similar to mitral cells than dGCs in terms of mGluR expression.

scholarly journals Odor-evoked Increases in Olfactory Bulb Mitral Cell Spiking Variability

iScience ◽  
2021 ◽  
pp. 102946
Author(s):  
Cheng Ly ◽  
Andrea K. Barreiro ◽  
Shree Hari Gautam ◽  
Woodrow L. Shew
Keyword(s):  
Olfactory Bulb ◽  
Mitral Cell

The Mitral and Short Axon Cells of the Olfactory Bulb

1970 ◽  
Vol 7 (3) ◽  
pp. 631-651
Author(s):  
J. L. PRICE ◽  
T. P. S. POWELL
Keyword(s):  
Electron Microscope ◽  
Olfactory Bulb ◽  
Granule Cells ◽  
Primary Dendrite ◽  
Plexiform Layer ◽  
Mitral Cell ◽  
Granule Cell Layer ◽  
Mitral Cells ◽  
Axon Initial Segments ◽  
Large Neurons

A description is given of the mitral and short axon cells of the olfactory bulb of the rat from Golgi material examined with the light microscope and from material examined with the electron microscope. The mitral cells are large neurons with primary and secondary dendrites which both extend into the overlying external plexiform layer, although only the primary dendrite enters the glomerular formations. No predominant antero-posterior orientation of the secondary dendrites has been found. Within the glomeruli the mitral cell dendrites are in synaptic contact with the olfactory nerves and also with the periglomerular cells, but elsewhere the only synapses on the mitral cells are the ‘reciprocal synapses’ with the granule cells. Synaptic-type vesicles are found in all parts of the mitral cells, including the axon initial segments; they appear to be especially concentrated in the distal portions of the dendrites. Several types of short axon cells have been found in the granule cell layer in Golgi-impregnated material. Their cell bodies can also be distinguished with the electron microscope, and from previous work it is probable that the axons of at least some of these cells form flattened-vesicle symmetrical synapses upon the granule cells.

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