Distribution of glycinergic neurons in the brain of glycine transporter-2 transgenic Tg(glyt2:Gfp) adult zebrafish: Relationship to brain-spinal descending systems

2012 ◽  
Vol 521 (2) ◽  
pp. 389-425 ◽  
Author(s):  
Antón Barreiro-Iglesias ◽  
Karolina Sandra Mysiak ◽  
Fátima Adrio ◽  
María Celina Rodicio ◽  
Catherina G. Becker ◽  
...  
Keyword(s):  
Adult Zebrafish ◽  
Glycine Transporter ◽  
Descending Systems ◽  
The Brain ◽  
Glycine Transporter 2

Exposure to aluminium causes behavioural alterations and oxidative stress in the brain of adult zebrafish

2021 ◽  
Vol 85 ◽  
pp. 103636
Author(s):  
Teresa Capriello ◽  
Luis M. Félix ◽  
Sandra M. Monteiro ◽  
Dércia Santos ◽  
Rita Cofone ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adult Zebrafish ◽  
The Brain ◽  
And Oxidative Stress

The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1

2004 ◽  
Vol 26 (4) ◽  
pp. 518-529 ◽  
Author(s):  
Koji Ohno ◽  
Michael Koroll ◽  
Oussama El Far ◽  
Petra Scholze ◽  
Jesus Gomeza ◽  
...  
Keyword(s):  
Pdz Domain ◽  
Glycine Transporter ◽  
Domain Protein ◽  
Glycine Transporter 2

Effects of chronic exposure to bisphenol-S on social behaviors in adult zebrafish: Disruption of the neuropeptide signaling pathways in the brain

2020 ◽  
Vol 262 ◽  
pp. 113992 ◽  
Author(s):  
Arash Salahinejad ◽  
Mohammad Naderi ◽  
Anoosha Attaran ◽  
Denis Meuthen ◽  
Som Niyogi ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Chronic Exposure ◽  
Social Behaviors ◽  
Adult Zebrafish ◽  
Bisphenol S ◽  
The Brain

scholarly journals Electrophysiological Recording in the Brain of Intact Adult Zebrafish

10.3791/51065 ◽  
2013 ◽  
Author(s):  
Lindsey Johnston ◽  
Rebecca E. Ball ◽  
Seth Acuff ◽  
John Gaudet ◽  
Andrew Sornborger ◽  
...  
Keyword(s):  
Electrophysiological Recording ◽  
Adult Zebrafish ◽  
The Brain

BDNF modulates glycine uptake in hippocampal synaptosomes by decreasing membrane insertion of glycine transporter 2

2016 ◽  
Vol 99 ◽  
pp. 94-102 ◽  
Author(s):  
Rita I. Aroeira ◽  
Sandra H. Vaz ◽  
Ana M. Sebastião ◽  
Cláudia A. Valente
Keyword(s):  
Membrane Insertion ◽  
Glycine Uptake ◽  
Glycine Transporter ◽  
Glycine Transporter 2

scholarly journals Behavioral Impairments and Oxidative Stress in the Brain, Muscle, and Gill Caused by Chronic Exposure of C70 Nanoparticles on Adult Zebrafish

2019 ◽  
Vol 20 (22) ◽  
pp. 5795 ◽  
Author(s):  
Sreeja Sarasamma ◽  
Gilbert Audira ◽  
Prabu Samikannu ◽  
Stevhen Juniardi ◽  
Petrus Siregar ◽  
...  
Keyword(s):  
High Dose ◽  
Adult Zebrafish ◽  
Activity Impairment ◽  
Skin Cells ◽  
Biochemical Assays ◽  
Muscle Tissues ◽  
Behavioral Impairments ◽  
Α Level ◽  
The Brain ◽  
Brain Tissues

There is an imperative need to develop efficient whole-animal-based testing assays to determine the potential toxicity of engineered nanomaterials. While previous studies have demonstrated toxicity in lung and skin cells after C70 nanoparticles (NPs) exposure, the potential detrimental role of C70 NPs in neurobehavior is largely unaddressed. Here, we evaluated the chronic effects of C70 NPs exposure on behavior and alterations in biochemical responses in adult zebrafish. Two different exposure doses were used for this experiment: low dose (0.5 ppm) and high dose (1.5 ppm). Behavioral tests were performed after two weeks of exposure of C70 NPs. We found decreased locomotion, exploration, mirror biting, social interaction, and shoaling activities, as well as anxiety elevation and circadian rhythm locomotor activity impairment after ~2 weeks in the C70 NP-exposed fish. The results of biochemical assays reveal that following exposure of zebrafish to 1.5 ppm of C70 NPs, the activity of superoxide dismutase (SOD) in the brain and muscle tissues increased significantly. In addition, the concentration of reactive oxygen species (ROS) also increased from 2.95 ± 0.12 U/ug to 8.46 ± 0.25 U/ug and from 0.90 ± 0.03 U/ug to 3.53 ± 0.64 U/ug in the muscle and brain tissues, respectively. Furthermore, an increased level of cortisol was also observed in muscle and brain tissues, ranging from 17.95 ± 0.90 pg/ug to 23.95 ± 0.66 pg/ug and from 3.47 ± 0.13 pg/ug to 4.91 ± 0.51 pg/ug, respectively. Increment of Hif1-α level was also observed in both tissues. The elevation was ranging from 11.65 ± 0.54 pg/ug to 18.45 ± 1.00 pg/ug in the muscle tissue and from 4.26 ± 0.11 pg/ug to 6.86 ± 0.37 pg/ug in the brain tissue. Moreover, the content of DNA damage and inflammatory markers such as ssDNA, TNF-α, and IL-1β were also increased substantially in the brain tissues. Significant changes in several biomarker levels, including catalase and malondialdehyde (MDA), were also observed in the gill tissues. Finally, we used a neurophenomic approach with a particular focus on environmental influences, which can also be easily adapted for other aquatic fish species, to assess the toxicity of metal and carbon-based nanoparticles. In summary, this is the first study to illustrate the adult zebrafish toxicity and the alterations in several neurobehavior parameters after zebrafish exposure to environmentally relevant amounts of C70 NPs.

scholarly journals Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis

2019 ◽  
Vol 2019 ◽  
pp. 1-17 ◽  
Author(s):  
Fuhai Bai ◽  
Yongyuan Ma ◽  
Haiyun Guo ◽  
Yuheng Li ◽  
Feifei Xu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Mast Cell ◽  
Knee Osteoarthritis ◽  
Dorsal Horn ◽  
Intrathecal Administration ◽  
Mast Cell Degranulation ◽  
Spinal Cord Dorsal Horn ◽  
Glycine Transporter ◽  
Mechanical Threshold ◽  
Glycine Transporter 2

The concept of “acupoint sensitization” refers to the functional status of acupoint switches from silent to active under pathological conditions. In clinic, acupoint sensitization provides important guidance for acupoints selection in different diseases. However, the mechanism behind this phenomenon remains unclear. We generated a model of knee osteoarthritis (KOA) by intra-articular injection of monosodium iodoacetate (MIA) into the left knee of rats. The paw withdrawal mechanical threshold (PWMT) and the total number of mast cells as well as mast cell degranulation rate (MCDR) of acupoint tissue were used to test whether the acupoints were sensitized. The results showed that KOA resulted in a reduced mechanical threshold and elevated total number of mast cell as well as mast cell degranulation rate at bilateral ST35 (Dubi) but not GB37 (Guangming) or nonacupoint area. The acupoint sensitization was accompanied by upregulation of glycine transporter 2 (GlyT2) and reduction of extracellular glycine levels in the bilateral dorsal horns of the spinal cord at L3-5. Selective inhibition of GlyT2 or intrathecal administration of glycine attenuated ST35 acupoint sensitization. The sensitization of bilateral ST35 was blocked after intraspinal GlyT2 short hairpin (sh) RNA (GlyT2-shRNA) microinjection to specifically downregulate GlyT2 expression in the left side (ipsilateral) L3-5 spinal cord dorsal horn before MIA injection. Moreover, electroacupuncture (EA) stimulation at ST35 ameliorated articular pathological lesions and improved KOA-related pain behaviors. GlyT2-shRNA injection reversed EA-induced pain relief but not EA-induced reduction of joint lesions. Overall, this study demonstrated that spinal GlyT2, especially elevated GlyT2 expression in the ipsilateral dorsal horn of the spinal cord, is a crucial mediator of ST35 acupoint sensitization in KOA rats.

scholarly journals Molecular Basis of the Dominant Negative Effect of a Glycine Transporter 2 Mutation Associated with Hyperekplexia

2014 ◽  
Vol 290 (4) ◽  
pp. 2150-2165 ◽  
Author(s):  
Esther Arribas-González ◽  
Jaime de Juan-Sanz ◽  
Carmen Aragón ◽  
Beatriz López-Corcuera
Keyword(s):  
Molecular Basis ◽  
Dominant Negative ◽  
Dominant Negative Effect ◽  
Glycine Transporter ◽  
Negative Effect ◽  
Glycine Transporter 2
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