Molecular and neuroanatomical characterization of single neurons in the mouse medullary gigantocellular reticular nucleus

2011 ◽  
Vol 519 (13) ◽  
pp. 2574-2593 ◽  
Author(s):  
E.M. Martin ◽  
N. Devidze ◽  
D.N. Shelley ◽  
L. Westberg ◽  
C. Fontaine ◽  
...  
Keyword(s):  
Reticular Nucleus ◽  
Single Neurons ◽  
Gigantocellular Reticular Nucleus

Spinohypothalamic Tract Neurons in the Cervical Enlargement of Rats: Locations of Antidromically Identified Ascending Axons and Their Collateral Branches in the Contralateral Brain

1997 ◽  
Vol 77 (1) ◽  
pp. 435-451 ◽  
Author(s):  
Ewa Kostarczyk ◽  
Xijing Zhang ◽  
Glenn J. Giesler
Keyword(s):  
Inferior Colliculus ◽  
Nucleus Ambiguus ◽  
Reticular Nucleus ◽  
Superior Olivary Complex ◽  
Lateral Reticular Nucleus ◽  
Antidromic Activation ◽  
Posterior Thalamus ◽  
Gigantocellular Reticular Nucleus ◽  
Medial Geniculate ◽  
Low Threshold

Kostarczyk, Ewa, Xijing Zhang, and Glenn J. Giesler, Jr. Spinohypothalamic tract neurons in the cervical enlargement of rats: locations of antidromically identified ascending axons and their collateral branches in the contralateral brain. J. Neurophysiol. 77: 435–451, 1997. Antidromic activation was used to determine the locations of ascending spinohypothalamic tract (SHT) axons and their collateral projections within C1, medulla, pons, midbrain, and caudal thalamus. Sixty-four neurons in the cervical enlargement were antidromically activated initially by stimulation within the contralateral hypothalamus. All but one of the examined SHT neurons responded either preferentially or specifically to noxious mechanical stimuli. A total of 239 low-threshold points was classified as originating from 64 ascending (or parent) SHT axons. Within C1, 38 ascending SHT axons were antidromically activated. These were located primarily in the dorsal half of the lateral funiculus. Within the medulla, the 29 examined ascending SHT axons were located ventrolaterally, within or adjacent to the lateral reticular nucleus or nucleus ambiguus. Within the pons, the 25 examined ascending SHT axons were located primarily surrounding the facial nucleus and the superior olivary complex. Within the caudal midbrain, the 23 examined SHT ascending axons coursed dorsally in a position adjacent to the lateral lemniscus. Within the anterior midbrain, SHT axons traveled rostrally near the brachium of the inferior colliculus. Within the posterior thalamus, all 17 examined SHT axons coursed rostrally through the posterior nucleus of thalamus. A total of 114 low-threshold points was classified as collateral branch points. Sixteen collateral branches were seen in C1; these were located primarily in the deep dorsal horn. Forty-five collateral branches were located in the medulla. These were primarily in or near the medullary reticular nucleus, nucleus ambiguus, lateral reticular nucleus, parvocellular reticular nucleus, gigantocellular reticular nucleus, cuneate nucleus, and the nucleus of the solitary tract. Twenty-six collateral branches from SHT axons were located in the pons. These were in the pontine reticular nucleus caudalis, gigantocellular reticular nucleus, parvocellular reticular nucleus, and superior olivary complex. Twenty-three collateral branches were located in the midbrain. These were in or near the mesencephalic reticular nucleus, brachium of the inferior colliculus, cuneiform nucleus, superior colliculus, central gray, and substantia nigra. In the caudal thalamus, two branches were in the posterior thalamic nucleus and two were in the medial geniculate. These results indicate that SHT axons ascend toward the hypothalamus in a clearly circumscribed projection in the lateral brain stem and posterior thalamus. In addition, large numbers of collaterals from SHT axons appear to project to a variety of targets in C1, the medulla, pons, midbrain, and caudal thalamus. Through its widespread collateral projections, the SHT appears to be capable of providing nociceptive input to many areas that are involved in the production of multifaceted responses to noxious stimuli.

Spinal input pathways affecting the medullary gigantocellular reticular nucleus

1983 ◽  
Vol 80 (3) ◽  
pp. 582-600 ◽  
Author(s):  
Nayef E. Saadé ◽  
Naman A. Salibi ◽  
Nabil R. Banna ◽  
Arnold L. Towe ◽  
Suhayl J. Jabbur
Keyword(s):  
Reticular Nucleus ◽  
Gigantocellular Reticular Nucleus

Cortical influence on single neurons of the lateral reticular nucleus of the cat

1970 ◽  
Vol 26 (2) ◽  
pp. 239-252 ◽  
Author(s):  
Peter Bruckmoser ◽  
Marie-Claude Hepp-Reymond ◽  
Mario Wiesendanger
Keyword(s):  
Reticular Nucleus ◽  
Lateral Reticular Nucleus ◽  
Single Neurons ◽  
Cortical Influence

scholarly journals Subcellular sequencing of single neurons reveals the dendritic transcriptome of GABAergic interneurons

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Julio D Perez ◽  
Susanne tom Dieck ◽  
Beatriz Alvarez-Castelao ◽  
Georgi Tushev ◽  
Ivy CW Chan ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Large Population ◽  
Cell Types ◽  
Mrna Localization ◽  
Gabaergic Neurons ◽  
Local Translation ◽  
Single Neurons ◽  
Cell Type ◽  
Cell Type Specific

Although mRNAs are localized in the processes of excitatory neurons, it is still unclear whether interneurons also localize a large population of mRNAs. In addition, the variability in the localized mRNA population within and between cell-types is unknown. Here we describe the unbiased transcriptomic characterization of the subcellular compartments of hundreds of single neurons. We separately profiled the dendritic and somatic transcriptomes of individual rat hippocampal neurons and investigated mRNA abundances in the soma and dendrites of single glutamatergic and GABAergic neurons. We found that, like their excitatory counterparts, interneurons contain a rich repertoire of ~4000 mRNAs. We observed more cell type-specific features among somatic transcriptomes than their associated dendritic transcriptomes. Finally, using cell-type specific metabolic labelling of isolated neurites, we demonstrated that the processes of Glutamatergic and, notably, GABAergic neurons were capable of local translation, suggesting mRNA localization and local translation is a general property of neurons.

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