Implementation of pigment-dispersing factor-immunoreactive neurons in a standardized atlas of the brain of the cockroach Leucophaea maderae

2010 ◽  
Vol 518 (20) ◽  
pp. 4113-4133 ◽  
Author(s):  
Hongying Wei ◽  
Basil el Jundi ◽  
Uwe Homberg ◽  
Monika Stengl
Keyword(s):  
Pigment Dispersing Factor ◽  
Leucophaea Maderae ◽  
The Brain

Myoinhibitory peptides in the brain of the cockroach Leucophaea maderae and colocalization with pigment-dispersing factor in circadian pacemaker cells

2012 ◽  
Vol 520 (5) ◽  
pp. 1078-1097 ◽  
Author(s):  
Julia Schulze ◽  
Susanne Neupert ◽  
Lilia Schmidt ◽  
Reinhard Predel ◽  
Tobias Lamkemeyer ◽  
...  
Keyword(s):  
Circadian Pacemaker ◽  
Pacemaker Cells ◽  
Pigment Dispersing Factor ◽  
Leucophaea Maderae ◽  
The Brain

scholarly journals Light Affects the Branching Pattern of Peptidergic Circadian Pacemaker Neurons in the Brain of the Cockroach Leucophaea maderae

2011 ◽  
Vol 26 (6) ◽  
pp. 507-517 ◽  
Author(s):  
Hongying Wei ◽  
Monika Stengl
Keyword(s):  
Fruit Flies ◽  
Branching Pattern ◽  
Circadian Pacemaker ◽  
Pacemaker Neurons ◽  
Optic Lobes ◽  
Pigment Dispersing Factor ◽  
Accessory Medulla ◽  
Leucophaea Maderae ◽  
The Brain

Pigment-dispersing factor–immunoreactive neurons anterior to the accessory medulla (aPDFMes) in the optic lobes of insects are circadian pacemaker neurons in cockroaches and fruit flies. The authors examined whether any of the aPDFMes of the cockroach Leucophaea maderae are sensitive to changes in period and photoperiod of light/dark (LD) cycles as a prerequisite to adapt to changes in external rhythms. Cockroaches were raised in LD cycles of 11:11, 13:13, 12:12, 6:18, or 18:6 h, and the brains of the adults were examined with immunocytochemistry employing antisera against PDF and orcokinin. Indeed, in 11:11 LD cycles, only the number of medium-sized aPDFMes specifically decreased, while it increased in 13:13. In addition, 18:6 LD cycles increased the number of large- and medium-sized aPDFMes, as well as the posterior pPDFMes, while 6:18 LD cycles only decreased the number of medium-sized aPDFMes. Furthermore, PDF-immunoreactive fibers in the anterior optic commissure and orcokinin-immunoreactive fibers in both the anterior and posterior optic commissures were affected by different lengths of light cycles. Thus, apparently different groups of the PDFMes, most of all the medium-sized aPDFMes, which colocalize orcokinin, respond to changes in period and photoperiod and could possibly allow for the adjustment to different photoperiods.

Localization of leucomyosuppressin in the brain and circadian clock of the cockroach Leucophaea maderae

2007 ◽  
Vol 328 (2) ◽  
pp. 443-452 ◽  
Author(s):  
Sandra Söhler ◽  
Susanne Neupert ◽  
Reinhard Predel ◽  
Ruthann Nichols ◽  
Monika Stengl
Keyword(s):  
Circadian Clock ◽  
Leucophaea Maderae ◽  
The Brain

scholarly journals Pigment-Dispersing Factor-expressing neurons convey circadian information in the honey bee brain

Open Biology ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 170224 ◽  
Author(s):  
Katharina Beer ◽  
Esther Kolbe ◽  
Noa B. Kahana ◽  
Nadav Yayon ◽  
Ron Weiss ◽  
...  
Keyword(s):  
Honey Bee ◽  
Pacemaker Cells ◽  
Compass Orientation ◽  
Sun Compass ◽  
Complex Behaviour ◽  
Pigment Dispersing Factor ◽  
Central Brain ◽  
The Brain ◽  
Clock Protein

Pigment-Dispersing Factor (PDF) is an important neuropeptide in the brain circadian network of Drosophila and other insects, but its role in bees in which the circadian clock influences complex behaviour is not well understood. We combined high-resolution neuroanatomical characterizations, quantification of PDF levels over the day and brain injections of synthetic PDF peptide to study the role of PDF in the honey bee Apis mellifera . We show that PDF co-localizes with the clock protein Period (PER) in a cluster of laterally located neurons and that the widespread arborizations of these PER/PDF neurons are in close vicinity to other PER-positive cells (neurons and glia). PDF-immunostaining intensity oscillates in a diurnal and circadian manner with possible influences for age or worker task on synchrony of oscillations in different brain areas. Finally, PDF injection into the area between optic lobes and the central brain at the end of the subjective day produced a consistent trend of phase-delayed circadian rhythms in locomotor activity. Altogether, these results are consistent with the hypothesis that PDF is a neuromodulator that conveys circadian information from pacemaker cells to brain centres involved in diverse functions including locomotion, time memory and sun-compass orientation.

Abundant distribution of locustatachykinin-like peptide in the nervous system and intestine of the cockroach Leucophaea maderae

1995 ◽  
Vol 348 (1326) ◽  
pp. 423-444 ◽  
Keyword(s):  
Nervous System ◽  
High Performance ◽  
Endocrine Cells ◽  
Muscle Layer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thoracic Ganglia ◽  
Leucophaea Maderae ◽  
Additional Cell ◽  
Frontal Ganglion ◽  
The Brain

An antiserum raised to the locust neuropeptide locustatachykinin I (LomTK I) was used for analysis of the distribution of tachykinin-related peptide in the cockroach Leucophaea maderae . Extracts of dissected brains, suboesophageal ganglia, thoracic ganglia and midguts were separated by high performance liquid chromatography and the fractions analysed in enzyme-linked immunosorbent assay with use of the LomTK antiserum. Each of the tissues was found to contain LomTK-like immunoreactive (LomTK-LI) components with retention times corresponding approximately to synthetic LomTK I and II and callitachykinins I and II. The LomTK antiserum was also used for immunocytochemical mapping of peptide in the nervous system and intestine of L.maderae . A large number of LomTK-LI interneurons were detected in the proto-, deuto- and tritocerebrum of the brain and in the suboesophaegeal ganglion. The immunoreactive neurons supply processes to most parts of the brain: the central body, protocerebral bridge, mushroom body calyces, antennal lobes, optic lobe and most regions of the non-glomerular neuropil. A few protocerebral neurons send LomTK-LI processes to the glandular lobe of the corpora cardiaca. In each of the thoracic ganglia there are six LomTK-LI interneurons and in each of the unfused abdominal ones there are two interneurons. The fused terminal ganglion contains some additional cell bodies in the posterior neuromers. LomTK-LI cell bodies were detected in the frontal ganglion and fibres were seen in this ganglion as well as in the hypocerebral ganglion. The frontal ganglion supplies LomTK-LI processes to the muscle layer of the pharynx. The muscle layer of the midgut is innervated by LomTK-LI fibres from the stomatogastric system (oesophageal nerve and associated ganglia). Additionally the midgut contains numerous LomTK-LI endocrine cells. A number of the pharyngeal dilator muscles were also found to be innervated by LomTK-LI fibres, probably derived from cell bodies in the suboesophageal ganglion. All the LomTK-LI neurons of the central nervous system appear to be interneurons, suggesting a neuromodulatory role of the endogenous tachykinins. The tachykinin-like peptides from peripheral ganglia may be involved in the control of foregut and midgut contractility and possibly the peptide of the endocrine cells in the midgut has additional actions related to intestinal function.

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