Ultrastructural analysis of the synaptic connectivity of TRPV1-expressing primary afferent terminals in the rat trigeminal caudal nucleus

2010 ◽  
Vol 518 (20) ◽  
pp. 4134-4146 ◽  
Author(s):  
Eun Jin Yeo ◽  
Yi Sul Cho ◽  
Sang Kyoo Paik ◽  
Atsushi Yoshida ◽  
Mae Ja Park ◽  
...  
Keyword(s):  
Ultrastructural Analysis ◽  
Synaptic Connectivity ◽  
Primary Afferent ◽  
Afferent Terminals ◽  
Trigeminal Caudal Nucleus

Ultrastructural Analysis of Ectopic Synaptic Boutons Arising From Peripherally Regenerated Primary Afferent Fibers

1999 ◽  
Vol 81 (4) ◽  
pp. 1636-1644 ◽  
Author(s):  
H. Richard Koerber ◽  
Karoly Mirnics ◽  
Anahid M. Kavookjian ◽  
Alan R. Light
Keyword(s):  
Dorsal Horn ◽  
Sensory Neurons ◽  
Ultrastructural Analysis ◽  
Peripheral Stimulus ◽  
Primary Sensory Neurons ◽  
Superficial Dorsal Horn ◽  
Primary Afferent ◽  
Afferent Fibers ◽  
Primary Afferent Fibers ◽  
Synaptic Boutons

Ultrastructural analysis of ectopic synaptic boutons arising from peripherally regenerated primary afferent fibers. The central axons of peripherally regenerated Aβ primary sensory neurons were impaled in the dorsal columns of α-chloralose-anesthetized cats 9–12 mo after axotomy. The adequate peripheral stimulus was determined, and the afferent fibers intracellularly stimulated while simultaneously recording the resulting cord dorsum potentials (CDPs). Fibers that successfully had reinnervated the skin responded to light tactile stimulation, and evoked CDPs that suggested dorsally located boutons were stained intracellularly with horseradish peroxidase (HRP). Two HRP-stained regenerated Aβ afferent fibers were recovered that supported large numbers of axon collaterals and swellings in laminae I, IIo, and IIi. Sections containing the ectopic collateral fibers and terminals in the superficial dorsal horn were embedded in plastic. Analyses of serial ultrathin sections revealed that ectopic projections from both regenerated fibers supported numerous synaptic boutons filled with clear round vesicles, a few large dense core vesicles (LDCVs) and several mitochondria (>3). All profiles examined in serial sections (19) formed one to three asymmetric axo-dendritic contacts. Unmyelinated portions of ectopic fibers giving rise to en passant and terminal boutons often contained numerous clear round vesicles. Several boutons (47%) received asymmetric contacts from axon terminals containing pleomorphic vesicles. These results strongly suggest that regenerated Aβ fibers activated by light tactile stimuli support functional connections in the superficial dorsal horn that have distinct ultrastructural features. In addition, the appearance of LDCVs suggests that primary sensory neurons are capable of changing their neurochemical phenotype.

scholarly journals BDNF released during neuropathic pain potentiates NMDA receptors in primary afferent terminals

2014 ◽  
Vol 39 (9) ◽  
pp. 1439-1454 ◽  
Author(s):  
Wenling Chen ◽  
Wendy Walwyn ◽  
Helena S. Ennes ◽  
Hyeyoung Kim ◽  
James A. McRoberts ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nmda Receptors ◽  
Primary Afferent ◽  
Afferent Terminals

Amino acid immunocytochemistry of primary afferent terminals in the rat dorsal horn

1994 ◽  
Vol 346 (2) ◽  
pp. 237-252 ◽  
Author(s):  
Juli G. Valtschanoff ◽  
Kris D. Phend ◽  
Patrizia S. Bernardi ◽  
Richard J. Weinberg ◽  
Aldo Rustioni
Keyword(s):  
Amino Acid ◽  
Dorsal Horn ◽  
Primary Afferent ◽  
Afferent Terminals
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