scholarly journals Ret-PCP2 colocalizes with protein kinase C in a subset of primate ON cone bipolar cells

2010 ◽  
Vol 518 (7) ◽  
pp. 1098-1112 ◽  
Author(s):  
Pyroja Sulaiman ◽  
Marie Fina ◽  
Rod Feddersen ◽  
Noga Vardi
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Bipolar Cells ◽  
Kinase C ◽  
Cone Bipolar Cells

Distribution of protein kinase C isoforms in the cat retina

2002 ◽  
Vol 19 (5) ◽  
pp. 549-562 ◽  
Author(s):  
BOZENA FYK-KOLODZIEJ ◽  
WENHUI CAI ◽  
ROBERTA G. POURCHO
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Ganglion Cells ◽  
Amacrine Cells ◽  
Bipolar Cells ◽  
Inner Plexiform Layer ◽  
Kinase C ◽  
Cat Retina ◽  
Rod Bipolar Cells ◽  
Cone Bipolar Cells

Immunocytochemical localization was carried out for five isoforms of protein kinase C (PKC) in the cat retina. In common with other mammalian species, PKCα was found in rod bipolar cells. Staining was also seen in a small population of cone bipolar cells with axon terminals ramifying near the middle of the inner plexiform layer (IPL). PKCβI was localized to rod bipolar cells, one class of cone bipolar cell, and numerous amacrine and displaced amacrine cells. Staining for PKCβII was seen in three types of cone bipolar cells as well as in amacrine and ganglion cells. Immunoreactivity for both PKCε and PKCζ was found in rod bipolar cells; PKCε was also seen in a population of cone bipolar cells and a few amacrine and ganglion cells whereas PKCζ was found in all ganglion cells. Double-label immunofluorescence studies showed that dendrites of the two PKCβII-positive OFF-cone bipolar cells exhibit immmunoreactivity for the kainate-selective glutamate receptor GluR5. The third PKCβII cone bipolar is an ON-type cell and did not stain for GluR5. The retinal distribution of these isoforms of PKC is consistent with a role in modulation of various aspects of neurotransmission including synaptic vesicle release and regulation of receptor molecules.

Differential staining of neurons in the human retina with antibodies to protein kinase C isozymes

1993 ◽  
Vol 10 (2) ◽  
pp. 341-351 ◽  
Author(s):  
Helga Kolb ◽  
Li Zhang ◽  
Laura Dekorver
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Plexiform Layer ◽  
Bipolar Cells ◽  
Wide Field ◽  
Human Retina ◽  
Axon Terminals ◽  
Kinase C ◽  
Pkc Β ◽  
Cone Bipolar Cells

AbstractMonoclonal antibodies to the three isozymes of protein kinase C (PKC) (α, β, and γ) were applied to postmortem human retina. Immunostaining was done on wholemount, or cryostat-sectioned retina, and visualized after ABC/DAB procedures by light (LM) and electron (EM) microscopy.The PCK-α antibody stained rod bipolar cells throughout the retina. EM analysis confirmed they were PKC-α-immunoreactive (IR) on their characteristic dendritic and axonal synaptology. Putative blue cone bipolar cells with wide-field axon terminals, stratifying in s5 of the inner plexiform layer (IPL), were also PKC-α-IR, and EM showed them to engage in narrow-cleft ribbon junctions in blue cone pedicles.The PKC-β antibody stained cone bipolar cells, many amacrine cells, and most ganglion cells. Cone bipolar cells were stained all the way into the foveal center: both midget and diffuse varieties were included. The IPL was densely PKC-IR and individual neurons could not be identified on stratification patterns. EM of the outer plexiform layer (OPL) revealed that both flat and invaginating cone bipolar types were IR and that IR axon terminals were presynaptic in all strata of the IPL. The occurrence of PKC-β-IR bipolar axons in stratum 2 of the IPL suggests that OFF-center as well as ON-center types were included.The PKC-γ antibody gave inferior staining compared with results from the other two antibodies; however, two varieties of wide-field monostratified amacrine cell and a large-bodied ganglion cell type were discernible.PKC in one form or another appears to be a second messenger used in neurotransmission by both rod and cone systems and ON- and OFF-center systems in the human retina.

scholarly journals Differential localization of protein kinase C isozymes in retinal neurons.

1991 ◽  
Vol 112 (6) ◽  
pp. 1241-1247 ◽  
Author(s):  
N Usuda ◽  
Y Kong ◽  
M Hagiwara ◽  
C Uchida ◽  
M Terasawa ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Bipolar Cells ◽  
Type I ◽  
Rabbit Retina ◽  
Type Ii ◽  
Retinal Neurons ◽  
Type Iii ◽  
Kinase C ◽  
Rod Bipolar Cells

We report the immunohistochemical localization of protein kinase C isozymes (types I, II, and III) in the rabbit retina using the monospecific monoclonal antibodies MC-1a, MC-2a, and MC-3a. Using immunoblot analysis of partially purified protein kinase C preparations of rabbit retina, types II and III isozymes alone were detected. The activity of type III was the stronger. By light microscopic immunohistochemical analysis, retinal neurons were negative for type I and positive for type II and type III isozymes. Type II was more diffusely distributed through the retinal layers, but was distinctive in ganglion cells, bipolar cells, and outer segments. The immunoreactivity was stronger for type III isozyme, and it was observed in mop (rod) bipolar cells and amacrine cells. By using immunoelectron microscopy, the cytoplasm of the cell body, the axon, and dendrites of the mop bipolar cells were strongly immunoreactive for type III. The so-called rod bipolar cells were for the first time seen to form synapses with rod photoreceptor cells. These differential localizations of respective isozymes in retinal neurons suggest that each isozyme has a different site of function in each neuron.

Protein kinase C localization in the synaptic terminal of rod bipolar cells

Neuroreport ◽  
1996 ◽  
Vol 7 (13) ◽  
pp. 2176-2180 ◽  
Author(s):  
Cecilia F. Vaquero ◽  
Almudena Velasco ◽  
Pedro de la Villa
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Bipolar Cells ◽  
Synaptic Terminal ◽  
Kinase C ◽  
Rod Bipolar Cells

Activation of protein kinase C potentiates transmitter release from goldfish retinal bipolar cells

1998 ◽  
Vol 31 ◽  
pp. S77
Author(s):  
Naotoshi Minami ◽  
Ken Berglund ◽  
Takeshi Sakaba ◽  
Masao Tachibana
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Transmitter Release ◽  
Bipolar Cells ◽  
Kinase C ◽  
Retinal Bipolar Cells

Localization of protein kinase C to UV-sensitive photoreceptors in the mouse retina

1998 ◽  
Vol 15 (1) ◽  
pp. 87-95 ◽  
Author(s):  
K.C. WIKLER ◽  
D.L. STULL ◽  
B.E. REESE ◽  
P.T. JOHNSON ◽  
E. BOGENMANN
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Regulatory Elements ◽  
Bipolar Cells ◽  
Mouse Retina ◽  
Transcriptional Regulatory Elements ◽  
Kinase C ◽  
Transcriptional Regulatory ◽  
Double Label ◽  
Rod Bipolar Cells

The present study has identified a population of cone photoreceptors in the murine retina that are uniquely immunoreactive for protein kinase C (PKC). Wavelength-sensitive cone subtypes are segregated along the dorso-ventral axis in the mouse retina with ventral retina occupied exclusively by ultraviolet wavelength-sensitive (UVWS) cones, and dorsal retina dominated by middle wavelength-sensitive cones. PKC-positive cones are found primarily in the ventral retina, and double-label immunocytochemistry using a short wavelength-sensitive opsin antibody confirms that they specifically correspond to the UVWS cone subtype. The PKC antibody, as documented in other mammals, also identifies rod bipolar cells in the mouse retina. UVWS cones and bipolar cells have previously been shown to share transcriptional regulatory elements, as observed in transgenic mice encoding a portion of the human SWS-opsin promoter controlling the lacZ reporter gene. In such mice, the transgene product, β-galactosidase, is expressed in populations of both cones and bipolar cells. The present study confirms that lacZ-expressing photoreceptors are indeed PKC-positive photoreceptors, but that the lacZ-expressing bipolar cells are not the PKC-positive rod bipolar cells. These cells must correspond to a type of cone bipolar cell.

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