Acute and chronic sectioning of fifth lumbar spinal nerve has equivalent effects on the primary afferents of sciatic nerve in rat spinal cord

2009 ◽  
Vol 517 (4) ◽  
pp. 481-492 ◽  
Author(s):  
Safa Aldeen S. Shehab
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Spinal Nerve ◽  
Primary Afferents ◽  
Rat Spinal Cord ◽  
Lumbar Spinal

scholarly journals Inhibition of the phosphoinositide 3-kinase-AKT-cyclic GMP-c-Jun N-terminal kinase signaling pathway attenuates the development of morphine tolerance in a mouse model of neuropathic pain

2021 ◽  
Vol 17 ◽  
pp. 174480692110033
Author(s):  
Travis Okerman ◽  
Taylor Jurgenson ◽  
Madelyn Moore ◽  
Amanda H Klein
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Signaling Pathway ◽  
Intracellular Signaling ◽  
Morphine Tolerance ◽  
Spinal Nerve ◽  
Intracellular Signaling Pathway ◽  
Spinal Cord Dorsal ◽  
Phosphoinositide 3 Kinase ◽  
Induced Tolerance

Research presented here sought to determine if opioid induced tolerance is linked to activity changes within the PI3Kγ-AKT-cGMP-JNK intracellular signaling pathway in spinal cord or peripheral nervous systems. Morphine or saline injections were given subcutaneously twice a day for five days (15 mg/kg) to male C57Bl/6 mice. A separate cohort of mice received spinal nerve ligation (SNL) one week prior to the start of morphine tolerance. Afterwards, spinal cord, dorsal root ganglia, and sciatic nerves were isolated for quantifying total and phosphorylated- JNK levels, cGMP, and gene expression analysis of Pik3cg, Akt1, Pten, and nNos1. This pathway was downregulated in the spinal cord with increased expression in the sciatic nerve of morphine tolerant and morphine tolerant mice after SNL. We also observed a significant increase in phosphorylated- JNK levels in the sciatic nerve of morphine tolerant mice with SNL. Pharmacological inhibition of PI3K or JNK, using thalidomide, quercetin, or SP600125, attenuated the development of morphine tolerance in mice with SNL as measured by thermal paw withdrawal. Overall, the PI3K/AKT intracellular signaling pathway is a potential target for reducing the development of morphine tolerance in the peripheral nervous system. Continued research into this pathway will contribute to the development of new analgesic drug therapies.

Regional blood flow in transected rat spinal cord during hypothermia

1990 ◽  
Vol 259 (6) ◽  
pp. H1649-H1654
Author(s):  
T. Sakamoto ◽  
A. Iwai ◽  
W. W. Monafo
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Sciatic Nerve ◽  
Rectal Temperature ◽  
Regional Blood Flow ◽  
Spinal Cord Transection ◽  
The Other ◽  
Rat Spinal Cord ◽  
Caudal Spinal Cord

Regional blood flow (RBF) increases in the spinal cord and sciatic nerve of acutely hypothermic rats. To determine whether cord transection affects this response, we measured RBF in rat spinal cord and sciatic nerve 2 h after cord transection at vertebrae T8 (n = 18 rats) and T11 (n = 18 rats) using [14C]butanol distribution. Nine in each group were normothermic controls. In T11 transection-hypothermia (25-27 degrees C rectal temperature), RBF increased in the three rostral cord segments by 28-40% (P less than 0.05); caudally, cord RBF was depressed in two segments (P less than 0.05), unchanged in the other; RBF fell in nerve (P less than 0.05). In T8 transection-hypothermia, RBF was unchanged in the two rostral cord segments; caudally, RBF was depressed in one cord segment (P less than 0.05) and unchanged in the others; RBF was unchanged in nerve. We conclude that RBF does not rise in caudal spinal cord segments or in sciatic nerve during hypothermia in rats with prior spinal cord transection.

Neonatal sciatic nerve transection induces TUNEL labeling of neurons in the rat spinal cord and DRG

Neuroreport ◽  
1997 ◽  
Vol 8 (13) ◽  
pp. 2837-2840 ◽  
Author(s):  
A L. R. Oliveira ◽  
M Risling ◽  
M Deckner ◽  
T Lindholm ◽  
F Langone ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Sciatic Nerve ◽  
Nerve Transection ◽  
Rat Spinal Cord ◽  
Sciatic Nerve Transection

mRNA and protein expression and activities of nitric oxide synthases in the lumbar spinal cord of neonatal rats after sciatic nerve transection and melatonin administration

2006 ◽  
Vol 407 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Fábio Rogério ◽  
Simone Aparecida Teixeira ◽  
Hamilton Jordão Júnior ◽  
Carla Cristina Judice Maria ◽  
André Schwambach Vieira ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Spinal Cord ◽  
Sciatic Nerve ◽  
Lumbar Spinal Cord ◽  
Nerve Transection ◽  
Neonatal Rats ◽  
Sciatic Nerve Transection ◽  
Melatonin Administration ◽  
Mrna And Protein Expression ◽  
Lumbar Spinal
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