Morphological properties of mouse retinal ganglion cells during postnatal development

2007 ◽  
Vol 503 (6) ◽  
pp. 803-814 ◽  
Author(s):  
Julie L. Coombs ◽  
Deborah Van Der List ◽  
Leo M. Chalupa
Keyword(s):  
Postnatal Development ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Morphological Properties ◽  
Retinal Ganglion

scholarly journals Cellular properties of intrinsically photosensitive retinal ganglion cells during postnatal development

2019 ◽  
Author(s):  
Jasmine A. Lucas ◽  
Tiffany M. Schmidt
Keyword(s):  
Postnatal Development ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
System Development ◽  
Retinal Development ◽  
Morphological Properties ◽  
Retinal Ganglion ◽  
Whole Cell Patch Clamp ◽  
Visual System Development ◽  
Retinofugal Projections

AbstractBackgroundMelanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) respond directly to light and have been shown to mediate a broad variety of visual behaviors in adult animals. ipRGCs are also the first light sensitive cells in the developing retina, and have been implicated in a number of retinal developmental processes such as pruning of retinal vasculature and refinement of retinofugal projections. However, little is currently known about the properties of the six ipRGC subtypes during development, and how these cells act to influence retinal development. We therefore sought to characterize the structure, physiology, and birthdate of the most abundant ipRGC subtypes, M1, M2, and M4, at discrete postnatal developmental timepoints.MethodsWe utilized whole cell patch clamp to measure the electrophysiological and morphological properties of ipRGC subtypes through postnatal development. We also used EdU labeling to determine the embryonic timepoints at which ipRGC subtypes terminally differentiate.ResultsOur data show that ipRGC subtypes are distinguishable from each other early in postnatal development. Additionally, we find that while ipRGC subtypes terminally differentiate at similar embryonic stages, the subtypes reach adult-like morphology and physiology at different developmental timepoints.ConclusionsThis work provides a broad assessment of ipRGC morphological and physiological properties during the postnatal stages at which they are most influential in modulating retinal development, and lays the groundwork for further understanding of the specific role of each ipRGC subtype in influencing retinal and visual system development.

Expression of Hsp27 in retinal ganglion cells of the rat during postnatal development

2004 ◽  
Vol 478 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Erin L. Hawkes ◽  
Anne Marie R. Krueger-Naug ◽  
Philip E.B. Nickerson ◽  
Tanya L. Myers ◽  
R. William Currie ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion

scholarly journals Subtype-dependent postnatal development of direction- and orientation-selective retinal ganglion cells in mice

2014 ◽  
Vol 112 (9) ◽  
pp. 2092-2101 ◽  
Author(s):  
Hui Chen ◽  
Xiaorong Liu ◽  
Ning Tian
Keyword(s):  
Postnatal Development ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Light Response ◽  
Orientation Selectivity ◽  
Mouse Retina ◽  
Retinal Ganglion ◽  
Tuning Width ◽  
Eye Opening ◽  
Synaptic Mechanisms

The direction-selective ganglion cells (DSGCs) and orientation-selective ganglion cells (OSGCs) encode the directional and the orientational information of a moving object, respectively. It is unclear how DSGCs and OSGCs mature in the mouse retina during postnatal development. Here we investigated the development of DSGCs and OSGCs after eye-opening. We show that 1) DSGCs and OSGCs are present at postnatal day 12 (P12), just before eye-opening; 2) the fractions of both DSGCs and OSGCs increase from P12 to P30; 3) the development of DSGCs and OSGCs is subtype dependent; and 4) direction and orientation selectivity are two separate features of retinal ganglion cells (RGCs) in the mouse retina. We classified RGCs into different functional subtypes based on their light response properties. Compared with P12, the direction and orientation selectivity of ON-OFF RGCs but not ON RGCs became stronger at P30. The tuning width of DSGCs for both ON and ON-OFF subtypes decreased with age. For OSGCs, we divided them into non-direction-selective (non-DS) OSGCs and direction-selective OSGCs (DS&OSGCs). For DS&OSGCs, we found that there was no correlation between the direction and orientation selectivity, and that the tuning width of both ON and ON-OFF subtypes remained unchanged with age. For non-DS OSGCs, the tuning width of ON but not ON-OFF subtype decreased with development. These findings provide a foundation to reveal the molecular and synaptic mechanisms underlying the development of the direction and orientation selectivity in the retina.

Large-scale morophological survey of rat retinal ganglion cells

2002 ◽  
Vol 19 (4) ◽  
pp. 483-493 ◽  
Author(s):  
WENZHI SUN ◽  
NING LI ◽  
SHIGANG HE
Keyword(s):  
Retinal Ganglion Cells ◽  
Large Scale ◽  
Ganglion Cells ◽  
Field Size ◽  
Morphological Properties ◽  
Cell Group ◽  
Retinal Ganglion ◽  
Dendritic Field ◽  
Coated Particle ◽  
Soma Size

Ganglion cells in an isolated wholemount preparation of the rat retina were labeled using the “DiOlistic” labeling method (Gan et al., 2000) and were classified according to their morphological properties. Tungsten particles coated with a lipophilic dye (DiI) were propelled into the wholemount retina using a gene gun. When a dye-coated particle contacted the cell membrane, the entire cell was labeled. The ganglion cells were classified into four types based on their soma size, dendritic-field size, branching pattern, and level of stratification. Broadly monostratified cells were classified into three types: RGA cells (large soma, large dendritic field); RGB cells (small- to medium-sized soma, small- to medium-sized dendritic field); and RGC cells (small- to medium-sized soma, medium-to-large dendritic field). Bistratified cells were classified as RGD. Several subtypes were identified within each ganglion cell group. A number of new subtypes were discovered and added into the existing catalog, among them were two types of bistratified cells. This study therefore represents the most complete morphological classification of rat retinal ganglion cells available to date.

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