Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain

2007 ◽  
Vol 502 (6) ◽  
pp. 1098-1108 ◽  
Author(s):  
Chiaki Ohtaka-Maruyama ◽  
Akiko Miwa ◽  
Hitoshi Kawano ◽  
Masataka Kasai ◽  
Haruo Okado
Keyword(s):  
Zinc Finger ◽  
Mouse Brain ◽  
Transcriptional Repressor ◽  
Temporal Expression

Only two of the five zinc fingers of the eukaryotic transcriptional repressor PRDI-BF1 are required for sequence-specific DNA binding

1992 ◽  
Vol 12 (5) ◽  
pp. 1940-1949
Author(s):  
A D Keller ◽  
T Maniatis
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Specific Binding ◽  
Regulatory Element ◽  
Zinc Fingers ◽  
Transcriptional Repressor ◽  
Gene Promoter ◽  
Binding Activity ◽  
Necessary And Sufficient ◽  
Sequence Requirements

The eukaryotic transcriptional repressor PRDI-BF1 contains five zinc fingers of the C2H2 type, and the protein binds specifically to PRDI, a 14-bp regulatory element of the beta interferon gene promoter. We have investigated the amino acid sequence requirements for specific binding to PRDI and found that the five zinc fingers and a short stretch of amino acids N terminal to the first finger are necessary and sufficient for PRDI-specific binding. The contribution of individual zinc fingers to DNA binding was investigated by inserting them in various combinations into another zinc finger-containing DNA-binding protein whose own fingers had been removed. We found that insertion of PRDI-BF1 zinc fingers 1 and 2 confer PRDI-binding activity on the recipient protein. In contrast, the insertion of PRDI-BF1 zinc fingers 2 through 5, the insertion of zinc finger 1 or 2 alone, and the insertion of zinc fingers 1 and 2 in reverse order did not confer PRDI-binding activity. We conclude that the first two PRDI-BF1 zinc fingers together are sufficient for the sequence-specific recognition of PRDI.

Lack of zinc finger protein 521 upregulates dopamine β-hydroxylase expression in the mouse brain, leading to abnormal behavior

Life Sciences ◽  
2019 ◽  
Vol 231 ◽  
pp. 116559
Author(s):  
Nobutaka Ohkubo ◽  
Mamoru Aoto ◽  
Kazunori Kon ◽  
Noriaki Mitsuda
Keyword(s):  
Zinc Finger ◽  
Mouse Brain ◽  
Zinc Finger Protein ◽  
Abnormal Behavior ◽  
Finger Protein

scholarly journals A Cys2/His2 Zinc Finger Protein Acts as a Repressor of the Green Revolution Gene SD1/OsGA20ox2 in Rice (Oryza sativa L.)

2020 ◽  
Author(s):  
Min Duan ◽  
Xiao-Juan Ke ◽  
Hong-Xia Lan ◽  
Xi Yuan ◽  
Peng Huang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Zinc Finger ◽  
Growth And Development ◽  
Zinc Finger Protein ◽  
Green Revolution ◽  
Critical Role ◽  
Transcriptional Repressor ◽  
Fine Tuning ◽  
Plant Growth And Development ◽  
Finger Protein

Abstract Gibberellins (GAs) play important roles in the regulation of plant growth and development. The green revolution gene SD1 encoding gibberellin 20-oxidase 2 (GA20ox2) has been widely used in modern rice breeding. However, the molecular mechanism of how SD1/OsGA20ox2 expression is regulated remains unclear. Here, we report a Cys2/His2 zinc finger protein ZFP207 acting as a transcriptional repressor of OsGA20ox2. ZFP207 was mainly accumulated in young tissues and more specifically in culm nodes. ZFP207-overexpression (ZFP207OE) plants displayed semidwarfism phenotype and small grains by modulating cell length. RNA interference of ZFP207 caused increased plant height and grain length. The application of exogenous GA3 could rescue the semidwarf phenotype of ZFP207OE rice seedlings. Moreover, ZFP207 repressed the expression of OsGA20ox2 via binding to its promoter region. Taken together, ZFP207 acts as a transcriptional repressor of SD1/OsGA20ox2 and it may play a critical role in plant growth and development in rice through the fine-tuning of GA biosynthesis .

scholarly journals Tobacco ZFT1, a Transcriptional Repressor with a Cys2/His2 Type Zinc Finger Motif that Functions in Spermine-Signaling Pathway

2005 ◽  
Vol 59 (3) ◽  
pp. 435-448 ◽  
Author(s):  
Yukiko Uehara ◽  
Yoshihiro Takahashi ◽  
Thomas Berberich ◽  
Atsushi Miyazaki ◽  
Hideki Takahashi ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Zinc Finger ◽  
Transcriptional Repressor ◽  
Zinc Finger Motif
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