Survival and death of mature avian motoneurons in organotypic slice culture: Trophic requirements for survival and different types of degeneration

2007 ◽  
Vol 501 (5) ◽  
pp. 669-690 ◽  
Author(s):  
Núria Brunet ◽  
Olga Tarabal ◽  
Manel Portero-Otín ◽  
Ronald W. Oppenheim ◽  
Josep E. Esquerda ◽  
...  
Keyword(s):  
Slice Culture ◽  
Organotypic Slice Culture ◽  
Different Types

Organotypic slice culture of the suprachiasmatic nucleus

1994 ◽  
Vol 18 (4) ◽  
pp. 601-604 ◽  
Author(s):  
Keiko Tominaga ◽  
Hitoshi Okamura ◽  
Shin-Ichi T. Inouye
Keyword(s):  
Suprachiasmatic Nucleus ◽  
Slice Culture ◽  
Organotypic Slice Culture

Synaptic Organization of an organotypic slice culture of the mammalian retina

1996 ◽  
Vol 13 (4) ◽  
pp. 759-771 ◽  
Author(s):  
Marco Sassoè-Pognetto ◽  
Andreas Feigenspan ◽  
Joachim Bormann ◽  
Heinz Wässle
Keyword(s):  
Hot Spots ◽  
Plexiform Layer ◽  
Slice Culture ◽  
Inner Plexiform Layer ◽  
Synaptic Organization ◽  
Glycine Receptors ◽  
Ribbon Synapses ◽  
Organotypic Slice Culture ◽  
Transmitter Receptors

AbstractVertical Slices of postnatal day 6 (P6) rat retina were cut and cultured using the roller-tube technique. The organotypic differentiation during a culture period of up to 30 days has been described in a previous study (Feigenspan et al., 1993a). Here we concentrated on the synaptic organization in the retinal slice culture. Electron microscopy revealed the presence of ribbon synapses in the outer plexiform layer and conventional and ribbon syanpses in the inner plexiform layer. Immunofluroscence with antibodies that recognize specific subunits of GABAA or glycine receptors revealed a punctuate distribution of the receptors. They were aggregated in “hot spots” that correspond to a concentration of receptors at postsynaptic sites. Different isoforms of GABAA and glycine receptors occured in the slice cultures. The experiments show that there is a differentiation of synapses and a diversity of transmitter receptors in the slice cultures that is comparable to the in vivo retina.

scholarly journals Heme oxygenase-1 contributes to pathology associated with thrombin-induced striatal and cortical injury in organotypic slice culture

2010 ◽  
Vol 1347 ◽  
pp. 170-178 ◽  
Author(s):  
Masatoshi Ohnishi ◽  
Hiroshi Katsuki ◽  
Kazuhiro Unemura ◽  
Yasuhiko Izumi ◽  
Toshiaki Kume ◽  
...  
Keyword(s):  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Slice Culture ◽  
Cortical Injury ◽  
Organotypic Slice Culture

scholarly journals Contribution of NPY Y5 Receptors to the Reversible Structural Remodeling Of Basolateral Amygdala Dendrites in Male Rats Associated with NPY-mediated Stress Resilience

2019 ◽  
Author(s):  
Sheldon D. Michaelson ◽  
Ana Pamela Miranda Tapia ◽  
Amanda McKinty ◽  
Heika Silveira Villarroel ◽  
James P. Mackay ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Structural Changes ◽  
Basolateral Amygdala ◽  
Excitatory Input ◽  
Male Rats ◽  
Repeated Treatment ◽  
Slice Culture ◽  
Stress Resilience ◽  
Organotypic Slice Culture ◽  
Vulnerability To Stress

ABSTRACTEndogenous neuropeptide Y (NPY) and corticotrophin-releasing factor (CRF) modulate the responses of the Basolateral amygdala (BLA) to stress, and are associated respectively with the development of stress resilience and vulnerability. We characterized the persistent effects of repeated NPY and CRF treatment on the structure and function of BLA principal neurons (PN) in a novel organotypic slice culture (OTC) model of rat BLA, and examined the contributions of specific NPY receptor subtypes to these neural and behavioral effects. In BLA principal neurons within the OTCs, repeated NPY treatment caused persistent attenuation of excitatory input and induced dendritic hypotrophy via Y5 receptors; conversely, CRF increased excitatory input and induced hypertrophy of BLA PNs. Repeated treatment of OTCs with NPY followed by an identical treatment with CRF, or vice versa inhibited or reversed all structural changes in OTCs. These structural responses to NPY or CRF required calcineurin or CaMKII, respectively. Finally, repeated intra-BLA injections of NPY or a Y5 receptor agonist increased social interaction and recapitulated structural changes in BLA neurons seen in OTCs, while a Y5 receptor antagonist prevented NPY’s effects both on behavior and on structure. These results implicate the Y5 receptor in the long-term, anxiolytic-like effects of NPY in the BLA, consistent with an intrinsic role in stress buffering, and highlight a remarkable mechanism by which BLA neurons may adapt to different levels of stress. Moreover, BLA OTCs offer a robust model to study mechanisms associated with resilience and vulnerability to stress in BLA.Significance StatementWithin the basolateral amygdala (BLA), Neuropeptide Y (NPY) is associated with buffering the neural stress response induced by CRF, and promoting stress resilience. We used a novel organotypic slice culture (OTC) model of BLA, complemented with in vivo studies, to examine the cellular mechanisms associated with the actions of NPY. In OTCs, repeated NPY treatment reduces the complexity of the dendritic extent of anxiogenic BLA principal neurons, making them less excitable. NPY, via activation of Y5 receptors, additionally inhibits and reverses the increases in dendritic extent and excitability induced by the stress hormone, corticotropin releasing factor (CRF). This NPY-mediated neuroplasticity indicates that resilience or vulnerability to stress may thus involve neuropeptide-mediated dendritic remodeling in BLA PNs.

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