Glycinergic neurons expressing enhanced green fluorescent protein in bacterial artificial chromosome transgenic mice

2004 ◽  
Vol 482 (2) ◽  
pp. 123-141 ◽  
Author(s):  
Hanns Ulrich Zeilhofer ◽  
Barbara Studler ◽  
Dimitrula Arabadzisz ◽  
Claude Schweizer ◽  
Seifollah Ahmadi ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mice ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Artificial Chromosome ◽  
Green Fluorescent

BAC transgenic mice express enhanced green fluorescent protein in central and peripheral cholinergic neurons

2006 ◽  
Vol 27 (3) ◽  
pp. 391-397 ◽  
Author(s):  
Yvonne N. Tallini ◽  
Bo Shui ◽  
Kai Su Greene ◽  
Ke-Yu Deng ◽  
Robert Doran ◽  
...  
Keyword(s):  
Nervous System ◽  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Peripheral Nervous System ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Cholinergic Neurons ◽  
Autonomic Nerves ◽  
Organ Systems ◽  
Green Fluorescent

The peripheral nervous system has complex and intricate ramifications throughout many target organ systems. To date this system has not been effectively labeled by genetic markers, due largely to inadequate transcriptional specification by minimum promoter constructs. Here we describe transgenic mice in which enhanced green fluorescent protein (eGFP) is expressed under the control of endogenous choline acetyltransferase (ChAT) transcriptional regulatory elements, by knock-in of eGFP within a bacterial artificial chromosome (BAC) spanning the ChAT locus and expression of this construct as a transgene. eGFP is expressed in ChATBAC-eGFP mice in central and peripheral cholinergic neurons, including cell bodies and processes of the somatic motor, somatic sensory, and parasympathetic nervous system in gastrointestinal, respiratory, urogenital, cardiovascular, and other peripheral organ systems. Individual epithelial cells and a subset of lymphocytes within the gastrointestinal and airway mucosa are also labeled, indicating genetic evidence of acetylcholine biosynthesis. Central and peripheral neurons were observed as early as 10.5 days postcoitus in the developing mouse embryo. ChATBAC-eGFP mice allow excellent visualization of all cholinergic elements of the peripheral nervous system, including the submucosal enteric plexus, preganglionic autonomic nerves, and skeletal, cardiac, and smooth muscle neuromuscular junctions. These mice should be useful for in vivo studies of cholinergic neurotransmission and neuromuscular coupling. Moreover, this genetic strategy allows the selective expression and conditional inactivation of genes of interest in cholinergic nerves of the central nervous system and peripheral nervous system.

Enhanced green fluorescent protein as a useful tag for rapid identification of homozygous transgenic mice

2001 ◽  
Vol 17 (3) ◽  
pp. 83-85 ◽  
Author(s):  
Masahiro Sato ◽  
Toshiteru Watanabe ◽  
Akiko Oshida ◽  
Ayako Nagashima ◽  
Jun-ichi Miyazaki ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Rapid Identification ◽  
Green Fluorescent

scholarly journals Visualization of lymphatic vessels by Prox1-promoter directed GFP reporter in a bacterial artificial chromosome-based transgenic mouse

Blood ◽  
2011 ◽  
Vol 117 (1) ◽  
pp. 362-365 ◽  
Author(s):  
Inho Choi ◽  
Hee Kyoung Chung ◽  
Swapnika Ramu ◽  
Ha Neul Lee ◽  
Kyu Eui Kim ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Bacterial Artificial Chromosome ◽  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Lymphatic Vessels ◽  
Artificial Chromosome ◽  
Transgenic Animal ◽  
Lymphatic Development ◽  
Vasculogenesis And Angiogenesis ◽  
Green Fluorescent

Abstract Although the blood vessel-specific fluorescent transgenic mouse has been an excellent tool to study vasculogenesis and angiogenesis, a lymphatic-specific fluorescent mouse model has not been established to date. Here we report a transgenic animal model that expresses the green fluorescent protein under the promoter of Prox1, a master control gene in lymphatic development. Generated using an approximately 200-kb-long bacterial artificial chromosome harboring the entire Prox1 gene, this Prox1-green fluorescent protein mouse was found to faithfully recapitulate the expression pattern of the Prox1 gene in lymphatic endothelial cells and other Prox1-expressing organs, and enabled us to conveniently visualize detailed structure and morphology of lymphatic vessels and networks throughout development. Our data demonstrate that this novel transgenic mouse can be extremely useful for detection, imaging, and isolation of lymphatic vessels and monitoring wound-associated lymphangiogenesis. Together, this Prox1-green fluorescent protein transgenic mouse will be a great tool for the lymphatic research.

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