Active caspase-3 expression during postnatal development of rat cerebellum is not systematically or consistently associated with apoptosis

2004 ◽  
Vol 476 (2) ◽  
pp. 154-173 ◽  
Author(s):  
Sowmini Oomman ◽  
Velvetlee Finckbone ◽  
Janet Dertien ◽  
Jennifer Attridge ◽  
William Henne ◽  
...  
Keyword(s):  
Postnatal Development ◽  
Caspase 3 ◽  
Rat Cerebellum ◽  
Active Caspase

Immunoreactivity of Ki-67/β-tubulin and immunocolocalization with active caspase-3 in rat dentate gyrus during postnatal development

2012 ◽  
Vol 46 (1-2) ◽  
pp. 10-18 ◽  
Author(s):  
Lirong Chang ◽  
Xuejuan Zhang ◽  
Weiya Liu ◽  
Yizhi Song ◽  
Xianghong Gao ◽  
...  
Keyword(s):  
Dentate Gyrus ◽  
Postnatal Development ◽  
Caspase 3 ◽  
Ki 67 ◽  
Β Tubulin ◽  
Active Caspase

Non-lethal active caspase-3 expression in Bergmann glia of postnatal rat cerebellum

2005 ◽  
Vol 160 (2) ◽  
pp. 130-145 ◽  
Author(s):  
Sowmini Oomman ◽  
Howard Strahlendorf ◽  
VelvetLee Finckbone ◽  
Jean Strahlendorf
Keyword(s):  
Caspase 3 ◽  
Bergmann Glia ◽  
Rat Cerebellum ◽  
Active Caspase

scholarly journals Effects of Mitochondrial Uncoupling Protein 2 Inhibition by Genipin in Human Cumulus Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Hongshan Ge ◽  
Fan Zhang ◽  
Dan Shan ◽  
Hua Chen ◽  
Xiaona Wang ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Uncoupling Protein ◽  
Physiological Role ◽  
Cumulus Cells ◽  
Mitochondrial Uncoupling ◽  
Mitochondrial Uncoupling Protein ◽  
Cultural Medium ◽  
Cellular Autophagy ◽  
Significant Difference ◽  
Active Caspase

UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues. But the exact roles of UCP2 in cumulus cells are still not clear. Genipin, a special UCP2 inhibitor, was added into the cultural medium to explore the roles of UCP2 in human cumulus cells. There were no significant differences in ATP and mitochondrial membrane potential levels in cumulus cells from UCP2 inhibiting groups as compared with the control. The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin. However, the ratio of reduced GSH to GSSG significantly declined after treatment with Genipin. UCP2 inhibition by Genipin also resulted in obvious increase in the active caspase-3, which accompanied the decline of caspase-3 mRNA. The level of progesterone in culture medium declined obviously after Genipin treatment. But there was no significant difference in estradiol concentrations. This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality.

Active caspase-3 is required for osteoclast differentiation

2006 ◽  
Vol 209 (3) ◽  
pp. 836-844 ◽  
Author(s):  
K.H. Szymczyk ◽  
T.A. Freeman ◽  
C.S. Adams ◽  
V. Srinivas ◽  
M.J. Steinbeck
Keyword(s):  
Caspase 3 ◽  
Osteoclast Differentiation ◽  
Active Caspase

Postnatal Development of the Acetylcholine System in Different Parts of the Rat Cerebellum

1982 ◽  
Vol 39 (6) ◽  
pp. 1726-1732 ◽  
Author(s):  
P. Kása ◽  
K. Bánsághy ◽  
Z. Rakonczay ◽  
K. Gulya
Keyword(s):  
Postnatal Development ◽  
Rat Cerebellum ◽  
Different Parts

scholarly journals Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Yuanyuan Wu ◽  
Junlin Zhang ◽  
Feng Liu ◽  
Cheng Yang ◽  
Yufang Zhang ◽  
...  
Keyword(s):  
Cyclosporine A ◽  
Caspase 3 ◽  
Interstitial Fibrosis ◽  
Ischemia Reperfusion ◽  
Inflammatory Cells ◽  
Protective Effects ◽  
Tubulointerstitial Damage ◽  
Apoptosis And Inflammation ◽  
The Right ◽  
Active Caspase

Ischemia reperfusion (IR) and cyclosporine A (CsA) injuries are unavoidable in kidney transplantation and are associated with allograft dysfunction. Herein, the effect and mechanism of a novel tissue protective peptide, helix B surface peptide (HBSP) derived from erythropoietin, were investigated in a rat model. The right kidney was subjected to 45 min ischemia, followed by left nephrectomy and 2-week reperfusion, with or without daily treatment of CsA 25 mg/kg and/or HBSP 8 nmol/kg. Blood urea nitrogen was increased by CsA but decreased by HBSP at 1 week and 2 weeks, while the same changes were revealed in urinary protein/creatinine only at 2 weeks. HBSP also significantly ameliorated tubulointerstitial damage and interstitial fibrosis, which were gradually increased by IR and CsA. In addition, apoptotic cells, infiltrated inflammatory cells, and active caspase-3+ cells were greatly reduced by HBSP in the both IR and IR + CsA groups. The 17 kD active caspase-3 protein was decreased by HBSP in the IR and IR + CsA kidneys, with decreased mRNA only in the IR + CsA kidneys. Taken together, it has been demonstrated, for the first time, that HBSP effectively improved renal function and tissue damage caused by IR and/or CsA, which might be through reducing caspase-3 activation and synthesis, apoptosis, and inflammation.

Changes of Ganglioside Pattern during Postnatal Development of Rat Cerebellum

1975 ◽  
Vol 3 (5) ◽  
pp. 696-698 ◽  
Author(s):  
GUY VINCENDON ◽  
M. SAÏD GHANDOUR ◽  
JACQUES ROBERT ◽  
GIORGIO GOMBOS ◽  
GERARD REBEL
Keyword(s):  
Postnatal Development ◽  
Rat Cerebellum

scholarly journals B Cell–Activating Factor Promotes B Cell Survival in Ectopic Lymphoid Tissues in Nasal Polyps

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhe-Zheng Wang ◽  
Jia Song ◽  
Hai Wang ◽  
Jing-Xian Li ◽  
Qiao Xiao ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Cell Survival ◽  
Stromal Cells ◽  
Caspase 3 ◽  
Lymphoid Tissues ◽  
B Cell Activating Factor ◽  
B Cell Survival ◽  
Active Caspase

Ectopic lymphoid tissues (eLTs) characterized by B cell aggregation contribute to the local immunoglobulin production in nasal polyps (NPs). B cell-activating factor (BAFF) is vital for B cell survival, proliferation, and maturation. The purpose of this study is to investigate whether BAFF is involved in the B cell survival and eLT formation in NPs. The mRNA and protein levels of BAFF in NP tissues with and without eLTs were detected by PCR and ELISA assay, respectively. The cellular sources of BAFF and active caspase-3-positive B cells in NPs were studied by immunofluorescence staining. B cells purified from NP tissues were stimulated with BAFF and were analyzed by flow cytometry. Stromal cells purified from NP tissues were stimulated with lymphotoxin (LT) α1β2, and BAFF levels in culture supernatants were analyzed by ELISA. Compared with those in control tissues and NPs without eLTs, the BAFF levels were elevated in NPs with eLTs. Abundant BAFF-positive cells and few active caspase-3-positive apoptotic B cells were found in NPs with eLTs, in contrast to those in NPs without eLTs. There was a negative correlation between the numbers of BAFF-positive cells and frequencies of apoptotic B cells in total B cells in NP tissues. BAFF protected nasal polyp B cells from apoptosis in vitro. Stromal cells were an important cellular source of BAFF in NPs with eLTs. LTα1β2 induced BAFF production from nasal stromal cells in vitro. We propose that BAFF contribute to eLT formation in NPs by promoting B cell survival.

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