Distribution of androgen and estrogen receptor mRNA in the brain and reproductive tissues of the leopard gecko,Eublepharis macularius

2001 ◽  
Vol 437 (4) ◽  
pp. 385-397 ◽  
Author(s):  
Turk Rhen ◽  
David Crews
Keyword(s):  
Estrogen Receptor ◽  
Receptor Mrna ◽  
Reproductive Tissues ◽  
Eublepharis Macularius ◽  
Leopard Gecko ◽  
Estrogen Receptor Mrna ◽  
The Brain

Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes

1990 ◽  
Vol 10 (12) ◽  
pp. 6674-6682
Author(s):  
E A McKenzie ◽  
N A Cridland ◽  
J Knowland
Keyword(s):  
Estrogen Receptor ◽  
Xenopus Oocytes ◽  
Liver Cells ◽  
Receptor Protein ◽  
Estrogen Receptor Protein ◽  
Receptor Mrna ◽  
Estrogen Receptor Mrna ◽  
Vitellogenin Gene ◽  
Vitellogenin Genes

We generate pure estrogen receptor protein in Xenopus oocytes by injecting them with estrogen receptor mRNA synthesized in vitro. A chromosomal vitellogenin gene, which normally responds to estrogen only in liver cells, is activated. Primer extension shows that initiation is accurate, and ribonuclease mapping shows that the first exon is correctly spliced out of the initial transcript. Long transcripts are produced, one being equal in length to poly(A)- vitellogenin mRNA. Immunochemical estimates of receptor levels in the oocyte nuclei suggest that pure receptor, acting alone, cannot activate oocyte vitellogenin genes unless unusually large amounts are present. However, when a receptor-free extract from liver cells is also injected, the amount of receptor required is reduced. Such an extract, but not pure receptor, can also activate albumin genes in oocytes.

scholarly journals Activation of chromosomal vitellogenin genes in Xenopus oocytes by pure estrogen receptor and independent activation of albumin genes.

1990 ◽  
Vol 10 (12) ◽  
pp. 6674-6682 ◽  
Author(s):  
E A McKenzie ◽  
N A Cridland ◽  
J Knowland
Keyword(s):  
Estrogen Receptor ◽  
Xenopus Oocytes ◽  
Liver Cells ◽  
Receptor Protein ◽  
Estrogen Receptor Protein ◽  
Receptor Mrna ◽  
Estrogen Receptor Mrna ◽  
Vitellogenin Gene ◽  
Vitellogenin Genes

We generate pure estrogen receptor protein in Xenopus oocytes by injecting them with estrogen receptor mRNA synthesized in vitro. A chromosomal vitellogenin gene, which normally responds to estrogen only in liver cells, is activated. Primer extension shows that initiation is accurate, and ribonuclease mapping shows that the first exon is correctly spliced out of the initial transcript. Long transcripts are produced, one being equal in length to poly(A)- vitellogenin mRNA. Immunochemical estimates of receptor levels in the oocyte nuclei suggest that pure receptor, acting alone, cannot activate oocyte vitellogenin genes unless unusually large amounts are present. However, when a receptor-free extract from liver cells is also injected, the amount of receptor required is reduced. Such an extract, but not pure receptor, can also activate albumin genes in oocytes.

scholarly journals Estrogen receptor mRNA expression patterns in the liver and ovary of female rainbow trout over a complete reproductive cycle

2012 ◽  
Vol 178 (3) ◽  
pp. 556-561 ◽  
Author(s):  
James J. Nagler ◽  
Timothy D. Cavileer ◽  
Joseph S. Verducci ◽  
Irvin R. Schultz ◽  
Sharon E. Hook ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Mrna Expression ◽  
Reproductive Cycle ◽  
Expression Patterns ◽  
Receptor Mrna ◽  
Estrogen Receptor Mrna
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