scholarly journals Immunohistochemical localization of Ih channel subunits, HCN1-4, in the rat brain

2004 ◽  
Vol 471 (3) ◽  
pp. 241-276 ◽  
Author(s):  
Takuya Notomi ◽  
Ryuichi Shigemoto
Keyword(s):  
Rat Brain ◽  
Immunohistochemical Localization ◽  
Ih Channel

Immunohistochemical localization of Ih channel HCN3 in the rat brain

2006 ◽  
Author(s):  
J. Liersch ◽  
C. Räder ◽  
T. Görcs ◽  
A. Scholten ◽  
E. Kremmer ◽  
...  
Keyword(s):  
Rat Brain ◽  
Immunohistochemical Localization ◽  
Ih Channel

Immunohistochemical Localization of GRF-Containing Neurons in Rat Brain

1986 ◽  
Vol 42 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Christine J. VandePol ◽  
John W. Leidy, Jr. ◽  
Thomas E. Finger ◽  
Richard J. Robbins
Keyword(s):  
Rat Brain ◽  
Immunohistochemical Localization

scholarly journals Immunohistochemical Localization of Lipocortin 1 in Rat Brain Is Sensitive to pH, Freezing, and Dehydration

1997 ◽  
Vol 45 (4) ◽  
pp. 527-538 ◽  
Author(s):  
James A. McKanna ◽  
Ming-Zhi Zhang
Keyword(s):  
Rat Brain ◽  
Protein Kinases ◽  
Brain Slice ◽  
Affinity Purification ◽  
Strong Acid ◽  
Immunohistochemical Localization ◽  
Molecular Properties ◽  
Annexin 1 ◽  
Contradictory Data

Lipocortin 1 (LC1, annexin 1) has received considerable attention as a substrate for protein kinases, as a Ca++- and phosphatidylserine-binding protein, and as a mediator of glucocorticoid anti-inflammatory effects. However, there has been confusion over localization of LC1 immunoreactivity (LC1-ir), which reportedly localizes to neurons and/or to astrocytes or microglia in rat brain. To test whether these contradictory data arise from unusual properties of the antigen, we developed a novel brain slice model to determine fixation and staining variables. The specificity of anti-LC1 sera was ensured by pre-absorption and affinity purification with immobilized recombinant LC1. Specific LC1-ir was detected in ramified microglia of brains perfused with acidified aldehydes and embedded in paraffin. However, commonly used immunohistochemical procedures have unexpected profound effects. LC1-ir was eliminated by fixation with neutral/alkaline aldehydes, by freezing before strong acid-aldehyde fixation, or by staining without partial de/rehydration before the primary serum. The sensitivity of LC1 epitopes to proton and water activities may reflect molecular properties important to LC1's roles in vivo. True LC1-ir was not detected in normal neurons or astrocytes.

Immunohistochemical localization of cytochrome P-450 in the rat brain

1988 ◽  
Vol 84 (2) ◽  
pp. 109-114 ◽  
Author(s):  
Christer Köhler ◽  
Lars G. Eriksson ◽  
Tiiu Hansson ◽  
Margaret Warner ◽  
Jan Åke-Gustafsson
Keyword(s):  
Rat Brain ◽  
Immunohistochemical Localization ◽  
Cytochrome P 450
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