Immunoreactivity against choline acetyltransferase, ?-aminobutyric acid, histamine, octopamine, and serotonin in the larval chemosensory system ofDosophila melanogaster

2002 ◽  
Vol 453 (2) ◽  
pp. 157-167 ◽  
Author(s):  
Fran�ois Python ◽  
Reinhard F. Stocker
Keyword(s):  
Choline Acetyltransferase ◽  
Aminobutyric Acid ◽  
Chemosensory System

scholarly journals Dietary Folic Acid and The Activity of Brain Cholinergic and γ-Aminobutyric Acid (GABA) Enzymes

1980 ◽  
Vol 7 (2) ◽  
pp. 133-134 ◽  
Author(s):  
M.I. Botez ◽  
Jocelyne Bachevalier ◽  
G. Tunnicliff
Keyword(s):  
Folic Acid ◽  
Choline Acetyltransferase ◽  
Glutamate Decarboxylase ◽  
Dietary Treatment ◽  
Folate Deficiency ◽  
Aminobutyric Acid ◽  
Male Rats ◽  
Folate Supplementation ◽  
Acetylcholine Metabolism ◽  
And Control

SUMMARY:Charles River CD male rats were randomly divided into 3 groups of five each and placed on folate deficient, folate excess, and control diets respectively. Glutamate decarboxylase GA D y-aminohutyrate aminotransferase (GABA-T), choline acetyltransferase (ChAc), and acetylcholinesterase (AChE) were assayed in the rat brains after 6 weeks of dietary treatment. Neither folate deficiency nor folate supplementation influenced the enzymes associated with GABA and acetylcholine metabolism.

scholarly journals A novel cholinergic neural pathway and its role in the drug relapse

2021 ◽  
Author(s):  
Teng He ◽  
Wenwen Chen ◽  
Yu Fan ◽  
Xing Xu ◽  
Zilin Wang ◽  
...  
Keyword(s):  
Food Intake ◽  
Conditioned Place Preference ◽  
Choline Acetyltransferase ◽  
Aminobutyric Acid ◽  
Central Nucleus ◽  
Parabrachial Nucleus ◽  
Neural Pathway ◽  
Lateral Parabrachial Nucleus ◽  
Drug Relapse ◽  
Cholinergic Projection

The lateral parabrachial nucleus (LPB) is critical hub implicated in the control of food intake, reward and aversion. Here, we identified a novel cholinergic projection from choline acetyltransferase (ChAT)-positive neurons in external portion of the lateral parabrachial nucleus (eLPBChAT) to γ-aminobutyric acid (GABA) neurons in central nucleus of amygdala (CeAGABA), activation of which could block methamphetamine (METH)-primed conditioned place preference (CPP) in mice.

Synthesis of acetylcholine by excitatory motoneurons in central nervous system of the leech

1977 ◽  
Vol 40 (2) ◽  
pp. 453-460 ◽  
Author(s):  
P. B. Sargent
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Choline Acetyltransferase ◽  
Cell Types ◽  
The Other ◽  
Aminobutyric Acid ◽  
Identified Neurons ◽  
Gamma Aminobutyric Acid ◽  
Motor Cells ◽  
Retzius Cells

1. A study was made of the synthesis of acetylcholine (ACh) and other transmitters by the cell bodies of functionally identified neurons in leech segmental ganglia. 2. Choline acetyltransferase, the synthetic enzyme for ACh, was detected in excitatory motoneurons but not in mechanosensory cells or Retzius cells. The ability of motoneurons to synthesize ACh was also demonstrated by their accumulation of [3H]ACh following incubation of segmental ganglia with [3H]choline. [3H]ACh was not detected in the other cell types. When eserine was included in [3H]choline incubations, the amount of [3H]ACh in motoneurons increased severalfold and small amounts of [3H]ACh (1% that in motor cells) appeared in extracts of sensory and Retzius cells. 3. In addition to [3H]ACh segmental ganglia synthesized [3H]5-HT, [3H]gamma-aminobutyric acid, [3H]dopamine, and [3H]octopamine from exogenous, labeled precursors. None of these labeled transmitters was detected in identified neurons except [3H]5-HT, which was found in Retzius cells. 4. These results provide biochemical evidence that excitatory motoneurons in the leech are cholinergic, but leave open the identity of the sensory transmitter(s).

Ultrastructural Localization of Acetylcholinesterase in the Arcuate Nucleus and Median Eminence of the Rat

1977 ◽  
Vol 35 ◽  
pp. 440-441
Author(s):  
K.A. Carson ◽  
C.B. Nemeroff ◽  
M.S. Rone ◽  
J.S. Kizer ◽  
J.S. Hanker
Keyword(s):  
Choline Acetyltransferase ◽  
Median Eminence ◽  
Arcuate Nucleus ◽  
Cholinergic Neurons ◽  
Ultrastructural Localization ◽  
Male Rats ◽  
Neonatal Rats ◽  
Good Marker ◽  
Chemical Studies ◽  
High Doses

Biochemical, physiological, pharmacological, and more recently enzyme histo- chemical data have indicated that cholinergic circuits exist in the hypothalamus. Ultrastructural correlates of these pathways such as acetylcholinesterase (AchE) positive neurons in the arcuate nucleus (ARC) and stained terminals in the median eminence (ME) have yet to be described. Initial studies in our laboratories utilizing chemical lesioning and microdissection techniques coupled with microchemical and light microscopic enzyme histo- chemical studies suggested the existence of cholinergic neurons in the ARC which project to the ME (1). Furthermore, in adult male rats with Halasz deafferentations (hypothalamic islands composed primarily of the isolated ARC and the ME) choline acetyltransferase (ChAc) activity, a good marker for cholinergic neurons, was not significantly reduced in the ME and was only somewhat reduced in the ARC (2). Treatment of neonatal rats with high doses of monosodium 1-glutamate (MSG) results in a lesion largely restricted to the neurons of the ARC.

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