scholarly journals Molecular characterization of 54 cases of false-negative fine-needle aspiration among 1347 papillary thyroid carcinomas

2014 ◽  
Vol 122 (10) ◽  
pp. 751-759 ◽  
Author(s):  
Agnese Proietti ◽  
Nicla Borrelli ◽  
Riccardo Giannini ◽  
Rossana Romani ◽  
Giancarlo Di Coscio ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Fine Needle Aspiration ◽  
False Negative ◽  
Needle Aspiration ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Thyroid Carcinomas

Reduction of False-Negative Papillary Thyroid Carcinomas by the Routine Analysis of BRAFT1799A Mutation on Fine-Needle Aspiration Biopsy Specimens

2012 ◽  
Vol 255 (5) ◽  
pp. 986-992 ◽  
Author(s):  
Marisa Cañadas-Garre ◽  
Patricia Becerra-Massare ◽  
Martín López de la Torre-Casares ◽  
Jesús Villar-del Moral ◽  
Susana Céspedes-Mas ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
False Negative ◽  
Needle Aspiration ◽  
Aspiration Biopsy ◽  
Routine Analysis ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Thyroid Carcinomas ◽  
Biopsy Specimens

scholarly journals MicroRNA expression profile helps to distinguish benign nodules from papillary thyroid carcinomas starting from cells of fine-needle aspiration

2012 ◽  
Vol 167 (3) ◽  
pp. 393-400 ◽  
Author(s):  
Patrizia Agretti ◽  
Eleonora Ferrarini ◽  
Teresa Rago ◽  
Antonio Candelieri ◽  
Giuseppina De Marco ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Mirna Expression ◽  
Thyroid Nodules ◽  
Expression Profiles ◽  
False Negative ◽  
Needle Aspiration ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Thyroid Carcinomas ◽  
Benign Nodules

ObjectiveMicroRNAs (miRNAs) are small endogenous noncoding RNAs that pair with target messengers regulating gene expression. Changes in miRNA levels occur in thyroid cancer. Fine-needle aspiration (FNA) with cytological evaluation is the most reliable tool for malignancy prediction in thyroid nodules, but cytological diagnosis remains undetermined for 20% of nodules.DesignIn this study, we evaluated the expression of seven miRNAs in benign nodules, papillary thyroid carcinomas (PTCs), and undetermined nodules at FNA.MethodsThe prospective study included 141 samples obtained by FNA of thyroid nodules from 138 patients. miRNA expression was evaluated by quantitative RT-PCR and statistical analysis of data was performed. Genetic analysis of codon 600 of BRAF gene was also performed.ResultsUsing data mining techniques, we obtained a criterion to classify a nodule as benign or malignant on the basis of miRNA expression. The decision model based on the expression of miR-146b, miR-155, and miR-221 was valid for 86/88 nodules with determined cytology (97.73%), and adopting cross-validation techniques we obtained a reliability of 78.41%. The prediction was valid for 31/53 undetermined nodules with 16 false-positive and six false-negative predictions. The mutated form V600E of BRAF gene was demonstrated in 19/43 PTCs and in 1/53 undetermined nodules.ConclusionsThe expression profiles of three miRNAs allowed us to distinguish benign from PTC starting from FNA. When the assay was applied to discriminate thyroid nodules with undetermined cytology, a low sensitivity and specificity despite the low number of false-negative predictions was obtained, limiting the practical interest of the method.

scholarly journals CHARACTERISTICS OF SUBCLONAL STRUCTURE IN THYROCYTE POPULATION IN RADIOIODINE-REFRACTORY METASTASES OF PAPILLARY THYROID CANCER

2019 ◽  
Vol 3 ◽  
pp. 3-9
Author(s):  
Anna Zelinskaya
Keyword(s):  
Thyroid Cancer ◽  
Papillary Thyroid Cancer ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Cystic Degeneration ◽  
Papillary Thyroid ◽  
Radioiodine Uptake ◽  
Fine Needle ◽  
Thyroid Carcinomas ◽  
Cellular Population

The aim of the study was cytological and immunocytochemical researches of thyrocyte populations in fine-needle aspiration smears of radioiodine-refractory metastases and their comparison with radioiodine-avid metastases and corresponding primary papillary carcinoma of the thyroid. Materials and Methods. The cytomorphological and immunocytochemical researches were conducted on the fine needle aspiration smears of 60 papillary thyroid carcinomas and 104 metastases, which were detected in the postoperative period. We applied the indirect immunoperoxidase technique using primary monoclonal mouse antibodies against leukocyte-common antigen, macrophage antigen, thyroglobulin, epithelial cell adhesion molecule, cytokeratines 7.8, polyclonal rabbit antibodies against calcitonin. Results. It was demonstrated, that radioiodine-avid metastases and corresponding primary papillary thyroid carcinoma smears had first type of cellular population with a relatively regular location of thyrocytes. Unlike them, radioiodine-refractory metastases smears had the second type of cellular population with irregularly location of thyrocytes among which showed up two cellular phenotypes. In our investigated smears some special cellular complexes were found – in 21 % of radioiodine-refractory metastases, 1 % in radioiodine-avid metastases and none of it in corresponding primary papillary thyroid. The cytological sign of cystic degeneration was found in 58 % of radioiodine-refractory metastases, but in radioiodine-avid metastases – was absent. Conclusion. The radioiodine-refractory metastases of papillary thyroid cancer demonstrated their distinction from radioiodine-uptake metastases with a presence of phenotypic heterogeneity. It is shown, that an appearance of certain cell subpopulations, special cellular complexes and cystic degeneration in fine-needle aspiration smears of radioiodine-refractory metastases, which in radioiodine-uptake metastases and corresponding primary papillary thyroid carcinomas were not found, can be used during the preoperative period to forecast the impossibility of radioiodine treatment.

BRAFV600Emutation in fine-needle aspiration aspirates: Association with poorer prognostic factors in larger papillary thyroid carcinomas

Head & Neck ◽  
2012 ◽  
Vol 35 (4) ◽  
pp. 548-553 ◽  
Author(s):  
Ji In Lee ◽  
Hye Won Jang ◽  
Sun Wook Kim ◽  
Jong-Won Kim ◽  
Young Lyun Oh ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Thyroid Carcinomas

scholarly journals Male sex, single nodularity, and young age are associated with the risk of finding a papillary thyroid cancer on fine-needle aspiration cytology in a large series of patients with nodular thyroid disease

2010 ◽  
Vol 162 (4) ◽  
pp. 763-770 ◽  
Author(s):  
T Rago ◽  
E Fiore ◽  
M Scutari ◽  
F Santini ◽  
G Di Coscio ◽  
...  
Keyword(s):  
Fine Needle Aspiration ◽  
Multiple Logistic Regression Analysis ◽  
False Negative ◽  
Needle Aspiration ◽  
Male Gender ◽  
Individual Risk ◽  
Papillary Thyroid ◽  
Fine Needle ◽  
Younger Age ◽  
Serum Tsh

ObjectiveTo evaluate the risk of papillary thyroid carcinoma (PTC) at fine-needle aspiration (FNA) cytology in 34 120 patients.ResultsFalse positive and false negative rates of FNA cytology were 1.2 and 1.8% in comparison with the histology in 3406 nodules from 3004 patients who underwent surgery. PTC (901 cases) was more frequent in solitary nodule (SN; 446/13 549, 3.3%) than in multinodular goiter (MNG; 411/19 923, 2%,χ2=48.8;P<0.0001), and in males (209/6382, 3.3%) than in females (648/26 945, 2.40%,χ2=15.58;P<0.0001). PTC prevalence in Graves' disease (GD; 13/286, 4.5%) and Hashimoto's thyroiditis (HT; 31/508, 6.1%) was higher than in SN, this difference being significant in HT (χ2=8.7;P=0.003), but not in GD (χ2=1.6;P=0.2).Using the multiple logistic regression analysis, independent risk predictors of PTC were determined, which were younger age (odds ratio (OR)=0.97, confidence interval (CI) 0.964–0.974;P<0.0001), male gender (OR=1.44, CI 1.231–1.683;P<0.0001), and SN versus MNG (OR=0.63, CI 0.547–0.717;P<0.0001). The individual risk predictivity was highly improved by including serum TSH in the prediction model, which was measured at FNA in 11 919 patients.ConclusionA cytology suspicious or indicative of PTC was associated with younger age, male gender, and solitary versus multiple nodularity. These clinical parameters, together with serum TSH, may allow formulation of an algorithm that could be usefully applied to predict the risk of PTC in individual patients when cytology does not give a diagnostic result.

Role of fine needle aspiration cytology in the preoperative diagnosis of malignancy in parotid and submandibular gland neoplasm

2014 ◽  
Vol 19 (2) ◽  
pp. 110-118
Author(s):  
Mohammad Amzad Hossain ◽  
Md Zahedul Alam ◽  
Md Rojibul Haque ◽  
Md Nazmul Haque ◽  
KM Nurul Alam ◽  
...  
Keyword(s):  
Submandibular Gland ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
False Negative ◽  
Needle Aspiration ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Medical College ◽  
Preoperative Diagnostic ◽  
Needle Aspiration Cytology

Objective: To evaluate the role of Fine Needle Aspiration Cytology in the preoperative diagnosis of malignancy in parotid and submandibular gland neoplasm. Methods: This cross sectional study on 50 cases was conducted in the Department of Otolaryngology and Head-Neck Surgery of Sir Salimullah Medical College Mitford Hospital and Dhaka Medical College Hospital from January’2009 to June 2010. Results: Fine needle aspiration cytology findings of our all 50 cases were compared with postoperative histopathological reports. Out of the 50 cases, in 36(72%) cases of benign neoplasm and 7(14%) cases of malignant neoplasm, pre-operative FNAC findings and post operative histopathological findings were same. In 7 cases, FNAC and post operative histopathological findings did not matched. These were 2 (4%) false positive and 5 (10%) false negative result.In our study sensitivity of FNAC for reporting malignancy was 58.33%, specificity to rule out malignancy was 94.73% and overall accuracy in detecting malignant tumour was 86%. Positive predictive value and negative value were 77.77% and 87.80% respectively. It can be concluded that fine needle aspiration cytology is a safe, cheap and useful preoperative diagnostic tool in the diagnosis of malignancy in parotid and submandibular gland, but as fine needle aspiration cytology partly depends on operator skill, it may give false negative and false positive result. Conclusion: FNAC is a useful preoperative diagnostic tool for malignant parotid and submandibular glands with high specificity and sensitivity. DOI: http://dx.doi.org/10.3329/bjo.v19i2.17634 Bangladesh J Otorhinolaryngol 2013; 19(2): 110-118

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