scholarly journals Molecular testing in lung cancer: Fine-needle aspiration specimen adequacy and test prioritization prior to the CAP/IASLC/AMP Molecular Testing Guideline publication

2014 ◽  
Vol 122 (6) ◽  
pp. 454-458 ◽  
Author(s):  
Oana C. Rafael ◽  
Mohamed Aziz ◽  
Harry Raftopoulos ◽  
Oana E. Vele ◽  
Weisheng Xu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Molecular Testing ◽  
Fine Needle ◽  
Test Prioritization

scholarly journals Improving Adequacy of Small Biopsy and Fine-Needle Aspiration Specimens for Molecular Testing by Next-Generation Sequencing in Patients With Lung Cancer: A Quality Improvement Study at Dartmouth-Hitchcock Medical Center

2016 ◽  
Vol 141 (3) ◽  
pp. 402-409 ◽  
Author(s):  
Vijayalakshmi Padmanabhan ◽  
Heather B. Steinmetz ◽  
Elizabeth J. Rizzo ◽  
Amber J. Erskine ◽  
Tamara L. Fairbank ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Next Generation Sequencing ◽  
Fine Needle Aspiration ◽  
Medical Center ◽  
Needle Aspiration ◽  
Molecular Testing ◽  
Next Generation ◽  
Fine Needle ◽  
Site Evaluation ◽  
Generation Sequencing

Context.— At our medical center, cytopathologists perform rapid on-site evaluation for specimen adequacy of fine-needle aspiration and touch imprint of needle core biopsy lung cancer samples. Two years ago the molecular diagnostics laboratory at our institution changed to next-generation sequencing using the Ion Torrent PGM and the 50-gene AmpliSeq Cancer Hotspot Panel v2 for analyzing mutations in a 50-gene cancer hot spot panel. This was associated with a dramatic fall in adequacy rate (68%). Objective.— To improve the adequacy rate to at least 90% for molecular testing using next-generation sequencing for all specimens collected by rapid on-site evaluation by the cytology laboratory. Design.— After baseline data on adequacy rate of cytology specimens with rapid on-site evaluation for molecular testing had been collected, 2 changes were implemented. Change 1 concentrated all the material in one block but did not produce desired results; change 2, in addition, faced the block only once with unstained slides cut up front for molecular testing. Data were collected in an Excel spreadsheet and adequacy rate was assessed. Results.— Following process changes 1 and 2 we reached our goal of at least 90% adequacy rate for molecular testing by next-generation sequencing on samples collected by rapid on-site evaluation including computed tomography–guided needle core biopsies (94%; 17 of 18) and fine-needle aspiration samples (94%; 30 of 32). Conclusion.— This study focused on factors that are controllable in a pathology department and on maximizing use of scant tissue. Optimizing the adequacy of the specimen available for molecular tests avoids the need for a second procedure to obtain additional tissue.

scholarly journals Molecular testing guidelines for lung adenocarcinoma: Utility of cell blocks and concordance between fine-needle aspiration cytology and histology samples

CytoJournal ◽  
2014 ◽  
Vol 11 ◽  
pp. 12 ◽  
Author(s):  
Jonas J. Heymann ◽  
William A. Bulman ◽  
Roger A. Maxfield ◽  
Charles A. Powell ◽  
Balazs Halmos ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Adenocarcinoma ◽  
Fine Needle Aspiration ◽  
Clinical Decision Making ◽  
Needle Aspiration ◽  
Molecular Techniques ◽  
Small Samples ◽  
Driver Mutations ◽  
Molecular Testing ◽  
Fine Needle

Background: Lung cancer is a leading cause of mortality, and patients often present at a late stage. More recently, advances in screening, diagnosing, and treating lung cancer have been made. For instance, greater numbers of minimally invasive procedures are being performed, and identification of lung adenocarcinoma driver mutations has led to the implementation of targeted therapies. Advances in molecular techniques enable use of scant tissue, including cytology specimens. In addition, per recently published consensus guidelines, cytology-derived cell blocks (CBs) are preferred over direct smears. Yet, limited comparison of molecular testing of fine-needle aspiration (FNA) CBs and corresponding histology specimens has been performed. This study aimed to establish concordance of epidermal growth factor receptor (EGFR) and Kirsten rat sarcoma (KRAS) virus homolog testing between FNA CBs and histology samples from the same patients. Materials and Methods: Patients for whom molecular testing for EGFR or KRAS was performed on both FNA CBs and histology samples containing lung adenocarcinoma were identified retrospectively. Following microdissection, when necessary, concordance of EGFR and KRAS molecular testing results between FNA CBs and histology samples was evaluated. Results: EGFR and/or KRAS testing was performed on samples obtained from 26 patients. Concordant results were obtained for all EGFR (22/22) and KRAS (17/17) mutation analyses performed. Conclusions: Identification of mutations in lung adenocarcinomas affects clinical decision-making, and it is important that results from small samples be accurate. This study demonstrates that molecular testing on cytology CBs is as sensitive and specific as that on histology.

Risk of malignancy and neoplasia predicted by three molecular testing platforms in indeterminate thyroid nodules on fine-needle aspiration

2019 ◽  
Vol 47 (9) ◽  
pp. 853-862 ◽  
Author(s):  
Kristen L. Partyka ◽  
Karen Trevino ◽  
Melissa L. Randolph ◽  
Harvey Cramer ◽  
Howard H. Wu
Keyword(s):  
Fine Needle Aspiration ◽  
Thyroid Nodules ◽  
Needle Aspiration ◽  
Molecular Testing ◽  
Fine Needle ◽  
Risk Of Malignancy

scholarly journals Transesophageal Ultrasound-Guided Fine-Needle Aspiration for the Mediastinal Restaging of Non-small Cell Lung Cancer

2011 ◽  
Vol 6 (9) ◽  
pp. 1510-1515 ◽  
Author(s):  
Martin B. von Bartheld ◽  
Michel I.M. Versteegh ◽  
Jerry Braun ◽  
Luuk N.A. Willems ◽  
Klaus F. Rabe ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Small Cell ◽  
Small Cell Lung ◽  
Ultrasound Guided ◽  
Fine Needle

Minimally Invasive Mediastinal Staging of Non–Small-Cell Lung Cancer: Emphasis on Ultrasonography-Guided Fine-Needle Aspiration

2014 ◽  
Vol 21 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Cynthia L. Harris ◽  
Eric M. Toloza ◽  
Jason B. Klapman ◽  
Shivakumar Vignesh ◽  
Kathryn Rodriguez ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Minimally Invasive ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Small Cell ◽  
Small Cell Lung ◽  
Fine Needle ◽  
Mediastinal Staging

scholarly journals A Case of Metastatic Lung Cancer Accompanied by Needle Tract Seeding after CT Guided Fine Needle Aspiration Biopsy.

Haigan ◽  
1994 ◽  
Vol 34 (6) ◽  
pp. 931-936
Author(s):  
Tadafumi Shimizu ◽  
Kensuke Ashina ◽  
Miwako Doi ◽  
Ritsuo Matsui ◽  
Kozo Sueyoshi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Biopsy ◽  
Needle Aspiration ◽  
Metastatic Lung Cancer ◽  
Ct Guided ◽  
Fine Needle ◽  
Needle Tract ◽  
Needle Tract Seeding ◽  
Metastatic Lung
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