scholarly journals Human pancreatic cancer fusion 2 (HPC2) 1-B3: A novel monoclonal antibody to screen for pancreatic ductal dysplasia

2012 ◽  
Vol 121 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Terry K. Morgan ◽  
Karin Hardiman ◽  
Christopher L. Corless ◽  
Sandra L. White ◽  
Robert Bonnah ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Monoclonal Antibody ◽  
Human Pancreatic Cancer

scholarly journals Human pancreatic cancer associated antigen detected by monoclonal antibody.

1986 ◽  
Vol 148 (2) ◽  
pp. 179-195 ◽  
Author(s):  
MASAO KOBARI ◽  
SEIKI MATSUNO ◽  
HIDEMI YAMAUCHI ◽  
TOSHIO SATO ◽  
TOSHIO KUDO ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Monoclonal Antibody ◽  
Human Pancreatic Cancer

scholarly journals Combining Fluorescent Cell Sorting and Single B Cell Amplification to Screen the Monoclonal Antibody Gene against Human Glypican-1 in Pancreatic Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Mi Huang ◽  
Yingying Ma ◽  
Xiaoyan Gao ◽  
Xinyang Li ◽  
Quan Ding ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Monoclonal Antibody ◽  
B Cell ◽  
Cell Sorting ◽  
Recombinant Antibody ◽  
Human Pancreatic Cancer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescent Cell ◽  
Novel Method ◽  
Antibody Gene

In this report, one novel method has been developed to screen the monoclonal antibody against human pancreatic cancer biomarker glypican-1 (GPC1) through the combination of fluorescent cell sorting and single B cell amplification. GPC1-positive B cells were sorted out from the peripheral blood mononuclear cells (PBMCs) by fluorescent cell sorting after the GPC1 immunization to the New Zealand white rabbit. Then, total RNA was extracted and reversely transcribed into cDNA, which was used as the template, and the variable region sequences of both heavy and light chains were amplified from the same B cell. Next, their recombinant antibody was expressed and purified from the human 293T cell after the antibody gene amplification and expression vector construction. The enzyme-linked immunosorbent assay (ELISA) and flow cytometry assays were used to determine the antibody affinity. The antibody named GPC-12 that we screened and obtained was proven to have natural heavy-light chain pairing information, and it was highly specific to the GPC1 antigen, and the affinity could reach 1 × 10−7 M. Overall, an effective and novel method has been successfully developed to screen the antibody by combining the fluorescent cell sorting and single-cell amplifying technologies, which was proved to be workable in our setting.

scholarly journals Radioimmunoscintigraphy of Human Pancreatic Cancer with 99mTc Labeled Monoclonal Antibody A7

1998 ◽  
Vol 31 (10) ◽  
pp. 2182-2182
Author(s):  
Hiroomi Matsumura ◽  
Eigo Otsuji ◽  
Shinichiro Kobayashi ◽  
Kazuma Okamoto ◽  
Kazuya Kitamura ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Monoclonal Antibody ◽  
Human Pancreatic Cancer ◽  
Monoclonal Antibody A7

Biodistribution of Murine and Chimeric Fab Fragments of the Monoclonal Antibody A7 in Human Pancreatic Cancer

Pancreas ◽  
1995 ◽  
Vol 10 (3) ◽  
pp. 265-273 ◽  
Author(s):  
Eigo Otsuji ◽  
Toshiharu Yamaguchi ◽  
Nobuki Yamaoka ◽  
Tatsuya Kotani ◽  
Makoto Kato ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Monoclonal Antibody ◽  
Human Pancreatic Cancer ◽  
Fab Fragments ◽  
Monoclonal Antibody A7
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