scholarly journals Reply to Genomic profiles of nasopharyngeal carcinoma: The importance of histological subtyping and Epstein-Barr virus in situ assays

Cancer ◽  
2017 ◽  
Vol 124 (2) ◽  
pp. 435-436 ◽  
Author(s):  
Siraj M. Ali ◽  
Jeffrey S. Ross ◽  
Kai Wang
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

In situ Detection of Epstein-Barr Virus Markers in Nasopharyngeal Carcinoma Patients

Oncology ◽  
1989 ◽  
Vol 46 (5) ◽  
pp. 310-317 ◽  
Author(s):  
Maria Li Lung ◽  
Kwok Hung Chan ◽  
Wai Pang Lam ◽  
Sio Kei Kou ◽  
Damon Choy ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
In Situ Detection

Epstein-Barr Virus in Nasopharyngeal Carcinoma of Guatemalan and Brazilian Patients

2016 ◽  
Vol 25 (4) ◽  
pp. 304-309 ◽  
Author(s):  
Alicia Rumayor Piña ◽  
Harim Tavares dos Santos ◽  
Roman Carlos ◽  
Albina Altemani ◽  
Oslei Paes de Almeida
Keyword(s):  
In Situ Hybridization ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

Background. Nasopharyngeal carcinoma (NPC) is highly associated with Epstein-Barr virus (EBV), particularly the undifferentiated nonkeratinizing subtype. Prevalence of EBV in NPC in countries such as Guatemala and Brazil has not been studied. Methods. We analyzed 19 cases of NPC, 11 from Guatemala and 8 from Brazil, for the presence of EBV by in situ hybridization and immunohistochemistry. Additionally, 19 hyperplastic adenoids from children were analyzed for EBV by in situ hybridization, 12 from Guatemala and 7 from Brazil. Results. All the NPC cases from Guatemala and 5 from Brazil were of the undifferentiated nonkeratinizing type. EBV-negative cases comprised 2 keratinizing NPC and 1 differentiated nonkeratinizing NPC. All undifferentiated nonkeratinizing NPC from both samples showed intense positivity for EBER, while LMP-1 only focally and scarcely expressed. EBER was positive in 75% and 43% of the adenoids from Guatemala and Brazil, respectively. Conclusions. All undifferentiated nonkeratinizing NPC irrespective of origin from Guatemala or Brazil were highly associated with EBV.

Analysis of Prognostic Factors of Nasopharyngeal Carcinoma: Impact of in Situ Hybridization for Epstein-Barr Virus Encoded Small RNA 1

2006 ◽  
Vol 134 (4) ◽  
pp. 639-645 ◽  
Author(s):  
Kazunari Nakao ◽  
Masato Mochiki ◽  
Ken-Ichi Nibu ◽  
Masashi Sugasawa ◽  
Hiroshi Uozaki
Keyword(s):  
Prognostic Factors ◽  
In Situ Hybridization ◽  
Nasopharyngeal Carcinoma ◽  
Small Rna ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Detection of Epstein-Barr Virus in Nasopharyngeal Carcinoma in Sudanese by <i>in Situ</i> Hybridization

2014 ◽  
Vol 05 (06) ◽  
pp. 517-522 ◽  
Author(s):  
Ameera A. M. Adam ◽  
Nazik E. Abdullah ◽  
Lamyaa A. M. El Hassan ◽  
Elwaleed M. Elamin ◽  
Muntaser E. Ibrahim ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Determination of the Expression of Latent Epstein Barr Virus in Omani Nasopharyngeal Carcinoma Patients

2021 ◽  
Vol 14 (1) ◽  
pp. 257-265
Author(s):  
Sheikha Nasser said Al-Shidhani ◽  
Shadia Al-Sinawi ◽  
Maiya Al-Bahri ◽  
Masoud Al-Kindi ◽  
Mohamed Mabruk
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Tissue Samples ◽  
Barr Virus ◽  
Carcinoma Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Epstein Barr ◽  
Formalin Fixed

Background: Nasopharyngeal carcinoma(NPC) is a rare malignant carcinoma that develops in the epithelial lining of the nasopharyngeal mucosa It is the most common neoplasm of the Nasopharynxand it is associated with many risk factors; one of them is Epstein-Barr virus infection. An Epstein-Barr virus is a tumorigenic herpes virus that infects and persists in B-lymphocytes without causing disease. This virus is associated with significant pathological conditions, such as benign and malignant lymphoproliferation. Objectives: To determine Epstein -Barr encoded RNA 1&2 (EBER1,2) and latent membrane protein (LMP) expressionin formalin-fixed paraffin-embedded tissue samples obtained from Omani patients diagnosed with nasopharyngeal carcinoma.Also, to identify the pattern and the type(s) of cells infected with EBV in nasopharyngeal carcinoma tissue samples obtained from Omani patients. Moreover, to compare the sensitivity of Immuno histochemistry and in-situ Hybridization for the detection of EBV in the nasopharyngeal carcinoma tissue sample. Materials and Method:Thirteen formalin-fixed paraffin-embedded nasopharyngeal carcinoma tissue samples archived from the period 2010 to 2017, were obtained from the Pathology Departments of Sultan Qaboos University Hospital and the Armed Force Hospital. These tissue samples were processed using two different methods Immunohistochemistry (IHC) and In situ hybridization (ISH). Results:Eleven out of thirteen NPC Omani patients were positive for EBV (84.61%) by either LMP-IHC or EBER-ISH. All cells stained positive for EBV in NPC tissue samples was of malignant type rather than normal cell type. EBV is mostly detected in patients in the age group of less than 50 years old. Also out of the 13 NPC patients, seven females (58.34%), and six males (46.15%) were positive for EBV. Conclusion:This study may provide evidence indicating an association between EBV and nasopharyngeal carcinoma. In addition, the detection of EBV in NPC obtained from Omani patients may encourage the physician to consider using anti-herpes virus drugs in the treatment of EBV positive NPC patients as an additional tool for the treatment of this kind of malignancy.

Detection of Epstein-Barr Virus in Nasopharyngeal Carcinoma by In Situ Hybridization and Polymerase Chain Reaction

1997 ◽  
Vol 107 (4) ◽  
pp. 523-526 ◽  
Author(s):  
Shigeyuki Murono ◽  
Tomokazu Yoshizaki ◽  
Saichiro Tanaka ◽  
Hajime Takeshita ◽  
Chang-Soo Park ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr
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