scholarly journals Identification of a gene-expression signature for predicting lymph node metastasis in patients with early stage cervical carcinoma

Cancer ◽  
2011 ◽  
Vol 117 (15) ◽  
pp. 3363-3373 ◽  
Author(s):  
Long Huang ◽  
Min Zheng ◽  
Qing-Min Zhou ◽  
Mei-Ying Zhang ◽  
Wei-Hua Jia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cervical Carcinoma ◽  
Early Stage ◽  
Gene Expression Signature ◽  
Expression Signature ◽  
Node Metastasis

Validation of a Gene Expression Signature for Assessment of Lymph Node Metastasis in Oral Squamous Cell Carcinoma

2012 ◽  
Vol 30 (33) ◽  
pp. 4104-4110 ◽  
Author(s):  
Sander R. van Hooff ◽  
Frank K.J. Leusink ◽  
Paul Roepman ◽  
Robert J. Baatenburg de Jong ◽  
Ernst-Jan M. Speel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Squamous Cell Carcinoma ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Early Stage ◽  
Gene Expression Signature ◽  
Expression Signature ◽  
Node Metastasis

Purpose Current assessment of lymph node metastasis in patients with head and neck squamous cell carcinoma is not accurate enough to prevent overtreatment. The aim of this study was validation of a gene expression signature for distinguishing metastasizing (N+) from nonmetastasizing (N0) squamous cell carcinoma of the oral cavity (OSCC) and oropharynx (OPSCC) in a large multicenter cohort, using a diagnostic DNA microarray in a Clinical Laboratory Improvement Amendments/International Organization for Standardization–approved laboratory. Methods A multigene signature, previously reported as predictive for the presence of lymph node metastases in OSCC and OPSCC, was first re-evaluated and trained on 94 samples using generic, whole-genome, DNA microarrays. Signature genes were then transferred to a dedicated diagnostic microarray using the same technology platform. Additional samples (n = 222) were collected from all head and neck oncologic centers in the Netherlands and analyzed with the diagnostic microarray. Human papillomavirus status was determined by real-time quantitative polymerase chain reaction. Results The negative predictive value (NPV) of the diagnostic signature on the entire validation cohort (n = 222) was 72%. The signature performed well on the most relevant subset of early-stage (cT1-T2N0) OSCC (n = 101), with an NPV of 89%. Conclusion Combining current clinical assessment with the expression signature would decrease the rate of undetected nodal metastases from 28% to 11% in early-stage OSCC. This should be sufficient to enable clinicians to refrain from elective neck treatment. A new clinical decision model that incorporates the expression signature is therefore proposed for testing in a prospective study, which could substantially improve treatment for this group of patients.

O53. Multicenter validation of a lymph node metastasis gene expression signature for head and neck squamous cell carcinomas

Oral Oncology ◽  
2011 ◽  
Vol 47 ◽  
pp. S46
Author(s):  
F.K.J. Leusink ◽  
P.J. Slootweg ◽  
M.W.M. van den Brekel ◽  
R.J. Baatenburg de Jong ◽  
F.C.P. Holstege ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Head And Neck ◽  
Squamous Cell ◽  
Gene Expression Signature ◽  
Squamous Cell Carcinomas ◽  
Expression Signature ◽  
Node Metastasis ◽  
Multicenter Validation

Gene Expression Signature and the Prediction of Lymph Node Metastasis in Colorectal Cancer by DNA Microarray

2009 ◽  
Vol 52 (12) ◽  
pp. 1941-1948 ◽  
Author(s):  
Toshiaki Watanabe ◽  
Takashi Kobunai ◽  
Toshiaki Tanaka ◽  
Soichiro Ishihara ◽  
Keiji Matsuda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Dna Microarray ◽  
Gene Expression Signature ◽  
Expression Signature ◽  
Node Metastasis
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