Fluorescence in situ hybridization analysis of aneuploidization patterns in monoclonal gammopathy of undetermined significance versus multiple myeloma and plasma cell leukemia

Cancer ◽  
2003 ◽  
Vol 97 (3) ◽  
pp. 601-609 ◽  
Author(s):  
Rasillo Ana ◽  
Tabernero María Dolores ◽  
Sánchez María Luz ◽  
Pérez de Andrés Martín ◽  
Martín Ayuso Marta ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Monoclonal Gammopathy ◽  
Hybridization Analysis ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia ◽  
Undetermined Significance

Cytogenetic and fluorescence in situ hybridization studies in 60 patients with multiple myeloma and plasma cell leukemia

2004 ◽  
Vol 148 (1) ◽  
pp. 71-76 ◽  
Author(s):  
Elisabet Lloveras ◽  
Isabel Granada ◽  
Lurdes Zamora ◽  
Blanca Espinet ◽  
Lourdes Florensa ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia

scholarly journals The Ig Heavy Chain Gene Is Frequently Involved in Chromosomal Translocations in Multiple Myeloma and Plasma Cell Leukemia as Detected by In Situ Hybridization

Blood ◽  
1997 ◽  
Vol 90 (2) ◽  
pp. 526-534 ◽  
Author(s):  
Kazuhiro Nishida ◽  
Akiko Tamura ◽  
Naozo Nakazawa ◽  
Yutaka Ueda ◽  
Tatsuo Abe ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Plasma Cell ◽  
Heavy Chain ◽  
Plasma Cell Leukemia ◽  
Critical Event ◽  
Chain Gene ◽  
Cell Leukemia ◽  
Ig Heavy Chain

Abstract Chromosome rearrangement of 14q32.33 has recurrently occurred with variable partner sites, including 11q13.3, 8q24.1, 18q21.3, and 6p21.1 in multiple myeloma (MM). To assess the actual incidence of 14q32.33 translocation and to elucidate its implication in the pathogenesis of MM, we studied 42 patients with MM, plasma cell leukemia, or plasmacytoma and 5 with monoclonal gammopathy with undetermined significance (MGUS) by G-banding and molecular cytogenetic methods. Using double-color fluorescence in situ hybridization (DCFISH) with 2 Ig heavy chain (IgH) gene probes, a yeast artificial chromosome (YAC) clone containing variable region, and a phage clone containing γ constant region, 14q32.33 translocation was detected as split signals of the IgH gene in 31 patients with plasma cell malignancies and 3 with MGUS. In contrast, of 40 patients who were assessed by G-banding, 3 (7.5%) showed the 14q+ chromosome. DCFISH detected a split of the IgH gene on interphase nuclei in 34 (73.9%) of 46 patients analyzed, whereas on metaphase spreads, it was in 22 (51.2%) of 43 patients analyzed. Interphase DCFISH was particularly useful to detect 14q32.33 translocation in 17 (65.4%) of 26 patients with normal karyotypes. Donor sites were identified in 11 of 22 patients demonstrated as carrying 14q32.33 translocation by metaphase FISH. Chromosome t(11; 14)(q13.3; q32.33) was detected in 5 patients, t(8; 14)(q24.1; q32.33) in 2, t(14; 18)(q32.33; q21.3) in 2, and t(7; 14)(q32.1; q32.33) in 1. A complex 14q32.33 translocation involving 3q and 16q24 was detected in 1 patient. Myeloma cells with t(7; 14) showed myelomonocytoid surface antigen. Because rearrangements of 14q32.33 were closely associated with translocation of proto-oncogenes into the IgH gene, our findings indicate that 14q32.33 translocation with various partner chromosomes is a critical event in the pathogenesis of MM and MGUS.

Cytogenetic and Fluorescence In Situ Hybridization Studies in Four Cases of Plasma Cell Leukemia

2000 ◽  
Vol 121 (2) ◽  
pp. 163-166 ◽  
Author(s):  
Elisabet Lloveras ◽  
Francesc Solé ◽  
Blanca Espinet ◽  
Carles Besses ◽  
Antoni Asensio ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Plasma Cell ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia

scholarly journals The Ig Heavy Chain Gene Is Frequently Involved in Chromosomal Translocations in Multiple Myeloma and Plasma Cell Leukemia as Detected by In Situ Hybridization

Blood ◽  
1997 ◽  
Vol 90 (2) ◽  
pp. 526-534 ◽  
Author(s):  
Kazuhiro Nishida ◽  
Akiko Tamura ◽  
Naozo Nakazawa ◽  
Yutaka Ueda ◽  
Tatsuo Abe ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Plasma Cell ◽  
Heavy Chain ◽  
Plasma Cell Leukemia ◽  
Critical Event ◽  
Chain Gene ◽  
Cell Leukemia ◽  
Ig Heavy Chain

Chromosome rearrangement of 14q32.33 has recurrently occurred with variable partner sites, including 11q13.3, 8q24.1, 18q21.3, and 6p21.1 in multiple myeloma (MM). To assess the actual incidence of 14q32.33 translocation and to elucidate its implication in the pathogenesis of MM, we studied 42 patients with MM, plasma cell leukemia, or plasmacytoma and 5 with monoclonal gammopathy with undetermined significance (MGUS) by G-banding and molecular cytogenetic methods. Using double-color fluorescence in situ hybridization (DCFISH) with 2 Ig heavy chain (IgH) gene probes, a yeast artificial chromosome (YAC) clone containing variable region, and a phage clone containing γ constant region, 14q32.33 translocation was detected as split signals of the IgH gene in 31 patients with plasma cell malignancies and 3 with MGUS. In contrast, of 40 patients who were assessed by G-banding, 3 (7.5%) showed the 14q+ chromosome. DCFISH detected a split of the IgH gene on interphase nuclei in 34 (73.9%) of 46 patients analyzed, whereas on metaphase spreads, it was in 22 (51.2%) of 43 patients analyzed. Interphase DCFISH was particularly useful to detect 14q32.33 translocation in 17 (65.4%) of 26 patients with normal karyotypes. Donor sites were identified in 11 of 22 patients demonstrated as carrying 14q32.33 translocation by metaphase FISH. Chromosome t(11; 14)(q13.3; q32.33) was detected in 5 patients, t(8; 14)(q24.1; q32.33) in 2, t(14; 18)(q32.33; q21.3) in 2, and t(7; 14)(q32.1; q32.33) in 1. A complex 14q32.33 translocation involving 3q and 16q24 was detected in 1 patient. Myeloma cells with t(7; 14) showed myelomonocytoid surface antigen. Because rearrangements of 14q32.33 were closely associated with translocation of proto-oncogenes into the IgH gene, our findings indicate that 14q32.33 translocation with various partner chromosomes is a critical event in the pathogenesis of MM and MGUS.

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