scholarly journals Ribosomal DNA methylation in patients with endometrial carcinoma

Cancer ◽  
2002 ◽  
Vol 94 (11) ◽  
pp. 2941-2952 ◽  
Author(s):  
Matthew A. Powell ◽  
David G. Mutch ◽  
Janet S. Rader ◽  
Thomas J. Herzog ◽  
Tim Hui-Ming Huang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Endometrial Carcinoma ◽  
Ribosomal Dna

The occurrence and transmission of a pattern of DNA methylation in Xenopus laevis ribosomal DNA

1978 ◽  
Vol 283 (997) ◽  
pp. 325-327 ◽  
Keyword(s):  
Dna Methylation ◽  
Xenopus Laevis ◽  
Ribosomal Dna ◽  
Functional Role ◽  
Helical Structure ◽  
Double Helical Structure ◽  
Sufficient Detail ◽  
Methyl Cytosine ◽  
Multicellular Organisms ◽  
Adenine Thymine

Even before the double helical structure of DNA had been put forward, it was known that DNA often contains bases in addition to adenine, thymine, guanine and cytosine (Wyatt 1951). In multicellular organisms the rare base is invariably 5-methyl cytosine (5MC), and few species that have been examined in sufficient detail have failed to show its presence (Shapiro 1968). We now know that 5MC arises by direct methylation of cytosine within the DNA duplex (Sneider & Potter 1969; Burdon & Adams 1969), and that in animals the methylated C is next to G on its 3' side (Grippo, Iaccarino, Parisi & Scarano 1968). Beyond this our knowledge is rather limited, and the functional role played by DNA methylation remains a complete mystery.

scholarly journals DNA methylation and DNA methyltransferase (DNMT1) activity pattern in endometrial carcinoma.

2016 ◽  
Vol 87 (01) ◽  
pp. 6-10 ◽  
Author(s):  
Katarzyna Romanek-Piva ◽  
Krzysztof Gałczyński ◽  
Aneta Adamiak-Godlewska ◽  
Tomasz Rechberger ◽  
Krzysztof Postawski
Keyword(s):  
Dna Methylation ◽  
Endometrial Carcinoma ◽  
Activity Pattern ◽  
Dna Methyltransferase

scholarly journals Ageing European lobsters ( Homarus gammarus ) using DNA methylation of evolutionarily conserved ribosomal DNA

2021 ◽  
Vol 14 (9) ◽  
pp. 2305-2318
Author(s):  
Eleanor A. Fairfield ◽  
David S. Richardson ◽  
Carly L. Daniels ◽  
Christopher L. Butler ◽  
Ewen Bell ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Ribosomal Dna ◽  
Homarus Gammarus ◽  
Evolutionarily Conserved

scholarly journals DNA-methylation dependent regulation of embryo-specific 5S ribosomal DNA cluster transcription in adult tissues of sea urchin Paracentrotus lividus

Genomics ◽  
2013 ◽  
Vol 102 (4) ◽  
pp. 397-402 ◽  
Author(s):  
Daniele Bellavia ◽  
Eufrosina Dimarco ◽  
Flores Naselli ◽  
Fabio Caradonna
Keyword(s):  
Dna Methylation ◽  
Sea Urchin ◽  
Ribosomal Dna ◽  
Paracentrotus Lividus ◽  
Adult Tissues

Ribosomal DNA methylation in a flax genotroph and a crown gall tumour

1987 ◽  
Vol 8 (3) ◽  
pp. 217-225 ◽  
Author(s):  
Keith S. Blundy ◽  
Christopher A. Cullis ◽  
Angus G. Hepburn
Keyword(s):  
Dna Methylation ◽  
Ribosomal Dna ◽  
Crown Gall ◽  
Crown Gall Tumour

Immunocytochemical localization of β1-4 galactosyltransferase in endometrial carcinoma cells

1990 ◽  
Vol 48 (3) ◽  
pp. 944-945
Author(s):  
Ichiro Yamamoto ◽  
Toshiaki Tachibana ◽  
Hiroko Maruyama ◽  
Noriyuki Komatsu ◽  
Hiroyuki Kuramoto ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Cell Lines ◽  
Endometrial Adenocarcinoma ◽  
Protein A ◽  
Rabbit Antiserum ◽  
Carcinoma Cells ◽  
Affinity Column ◽  
Antibody Technique ◽  
Galactosyl Transferase ◽  
C Terminus

We have paid attention to the alteration of glycosyltransferase in carcinoma cells, because it might be related to the malignancy of the cells. In this connection, localization of β1-4 galactosyl transferase (β1-4 Gal T) in human endometrial carcinoma cells was examined immunocytochemically using two kinds of cell lines, each of which showed different degree of differentiation.An antibody was purified from the rabbit antiserum against the synthetic peptide, IFNRLVFRGMSC (W89) of human β1-4 Gal T coupled with KLH (keyhole limpet hemocyanine) by protein A column and peptide-affinity column chromatography. The anti-W89 serum reacts to the C-terminus of human β 1-4 Gal T and to both membrane-bound and soluble forms of the enzyme. Cell line of well differentiated endometrial adenocarcinoma (I) and that of poorly differentiated endometrial adenocarcinoma (50B) were cultivated respectively in MEM medium containing 15% FCS and 2 mM glutamine for 4 d at 37°C under 5% CO2. The cells were fixed in a mixture of 4% paraformaldehyde and 0.1% glutaraldehyde in 0.1 M Soerensen’s phosphate buffer (pH 7.4) at 4°C for 30 min, washed with PBS, then freezed and thawed. The indirect method of the peroxidase- labeled antibody technique was used for immunocytochemistry of both LM and TEM on the cell lines. The cells were dehydrated in ethanol and embedded in TAAB 812. Ultrathin sections were observed under a TEM, JEM-100S.

DNA methylation in satellite repeats disorders

2019 ◽  
Vol 63 (6) ◽  
pp. 757-771 ◽  
Author(s):  
Claire Francastel ◽  
Frédérique Magdinier
Keyword(s):  
Dna Methylation ◽  
Human Genome ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Satellite Repeats ◽  
Tremendous Progress ◽  
Genes Encoding ◽  
Dna Elements ◽  
Near Future

Abstract Despite the tremendous progress made in recent years in assembling the human genome, tandemly repeated DNA elements remain poorly characterized. These sequences account for the vast majority of methylated sites in the human genome and their methylated state is necessary for this repetitive DNA to function properly and to maintain genome integrity. Furthermore, recent advances highlight the emerging role of these sequences in regulating the functions of the human genome and its variability during evolution, among individuals, or in disease susceptibility. In addition, a number of inherited rare diseases are directly linked to the alteration of some of these repetitive DNA sequences, either through changes in the organization or size of the tandem repeat arrays or through mutations in genes encoding chromatin modifiers involved in the epigenetic regulation of these elements. Although largely overlooked so far in the functional annotation of the human genome, satellite elements play key roles in its architectural and topological organization. This includes functions as boundary elements delimitating functional domains or assembly of repressive nuclear compartments, with local or distal impact on gene expression. Thus, the consideration of satellite repeats organization and their associated epigenetic landmarks, including DNA methylation (DNAme), will become unavoidable in the near future to fully decipher human phenotypes and associated diseases.

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