scholarly journals Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies

2012 ◽  
Vol 7 (3) ◽  
pp. 302-307 ◽  
Author(s):  
P. Danhier ◽  
G. De Preter ◽  
S. Boutry ◽  
I. Mahieu ◽  
P. Leveque ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Iron Oxide ◽  
Cell Labeling ◽  
Oxide Content ◽  
Iron Oxide Content ◽  
Paramagnetic Resonance ◽  
Sensitive Tool ◽  
Electron Paramagnetic ◽  
In Cells

scholarly journals Concentration of Fe(3+)-Triapine in BEAS-2B Cells

2019 ◽  
Vol 20 (12) ◽  
pp. 3062 ◽  
Author(s):  
William E. Antholine ◽  
Charles R. Myers
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Tumor Cells ◽  
Iron Uptake ◽  
Antitumor Agent ◽  
Epr Spectrum ◽  
Paramagnetic Resonance ◽  
Epr Method ◽  
First Time ◽  
Electron Paramagnetic ◽  
In Cells

An electron paramagnetic resonance (EPR) method was used to determine the concentration of the antitumor agent Triapine in BEAS-2B cells when Triapine was bound to iron (Fe). Knowledge of the concentration of Fe-Triapine in tumor cells may be useful to adjust the administration of the drug or to adjust iron uptake in tumor cells. An EPR spectrum is obtained for Fe(3+)-Triapine, Fe(3+)(Tp)2+, in BEAS-2B cells after addition of Fe(3+)(Tp)2+. Detection of the low spin signal for Fe(3+)(Tp)2+ shows that the Fe(3+)(Tp)2+ complex is intact in these cells. It is proposed that Triapine acquires iron from transferrin in cells including tumor cells. Here, it is shown that iron from purified Fe-transferrin is transferred to Triapine after the addition of ascorbate. To our knowledge, this is the first time that the EPR method has been used to determine the concentration of an iron antitumor agent in cells.

Electron paramagnetic resonance as an effective method for a characterization of functionalized iron oxide

2014 ◽  
Vol 75 (5) ◽  
pp. 594-598 ◽  
Author(s):  
Bernadeta Dobosz ◽  
Ryszard Krzyminiewski ◽  
Grzegorz Schroeder ◽  
Joanna Kurczewska
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Iron Oxide ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

scholarly journals Iron Oxide Nanoparticle Uptake in Mouse Brachiocephalic Artery Atherosclerotic Plaque Quantified by T2-Mapping MRI

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 279
Author(s):  
Rik P. M. Moonen ◽  
Bram F. Coolen ◽  
Judith C. Sluimer ◽  
Mat J. A. P. Daemen ◽  
Gustav J. Strijkers
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Iron Oxide ◽  
Atherosclerotic Plaque ◽  
Ex Vivo ◽  
T2 Mapping ◽  
Atherosclerotic Plaques ◽  
Brachiocephalic Artery ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

The purpose of our study was to monitor the iron oxide contrast agent uptake in mouse brachiocephalic artery (BCA) atherosclerotic plaques in vivo by quantitative T2-mapping magnetic resonance imaging (MRI). Female ApoE−/− mice (n = 32) on a 15-week Western-type diet developed advanced plaques in the BCA and were injected with ultra-small superparamagnetic iron oxides (USPIOs). Quantitative in vivo MRI at 9.4 T was performed with a Malcolm-Levitt (MLEV) prepared T2-mapping sequence to monitor the nanoparticle uptake in the atherosclerotic plaque. Ex vivo histology and particle electron paramagnetic resonance (pEPR) were used for validation. Longitudinal high-resolution in vivo T2-value maps were acquired with consistent quality. Average T2 values in the plaque decreased from a baseline value of 34.5 ± 0.6 ms to 24.0 ± 0.4 ms one day after injection and partially recovered to an average T2 of 27 ± 0.5 ms after two days. T2 values were inversely related to iron levels in the plaque as determined by ex vivo particle electron paramagnetic resonance (pEPR). We concluded that MRI T2 mapping facilitates a robust quantitative readout for USPIO uptake in atherosclerotic plaques in arteries near the mouse heart.

scholarly journals Tracking Conformational Changes in Calmodulin in vitro, in Cell Extract, and in Cells by Electron Paramagnetic Resonance Distance Measurements

ChemPhysChem ◽  
2019 ◽  
Vol 20 (14) ◽  
pp. 1860-1868 ◽  
Author(s):  
Arina Dalaloyan ◽  
Andrea Martorana ◽  
Yoav Barak ◽  
Diana Gataulin ◽  
Eitan Reuveny ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Conformational Changes ◽  
Cell Extract ◽  
Distance Measurements ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic ◽  
In Cells
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