scholarly journals Identification of Broad-Spectrum Dengue/Zika Virus Replication Inhibitors by Functionalization of Quinoline and 2,6-Diaminopurine Scaffolds

ChemMedChem ◽  
2018 ◽  
Vol 13 (14) ◽  
pp. 1371-1376 ◽  
Author(s):  
Suzanne J. F. Kaptein ◽  
Paolo Vincetti ◽  
Emmanuele Crespan ◽  
Jorge I. Armijos Rivera ◽  
Gabriele Costantino ◽  
...  
Keyword(s):  
Virus Replication ◽  
Broad Spectrum ◽  
Zika Virus

scholarly journals Cover Feature: Identification of Broad-Spectrum Dengue/Zika Virus Replication Inhibitors by Functionalization of Quinoline and 2,6-Diaminopurine Scaffolds (ChemMedChem 14/2018)

ChemMedChem ◽  
2018 ◽  
Vol 13 (14) ◽  
pp. 1370-1370
Author(s):  
Suzanne J. F. Kaptein ◽  
Paolo Vincetti ◽  
Emmanuele Crespan ◽  
Jorge I. Armijos Rivera ◽  
Gabriele Costantino ◽  
...  
Keyword(s):  
Virus Replication ◽  
Broad Spectrum ◽  
Zika Virus ◽  
Feature Identification

The P-MAPA immunomodulator partially prevents apoptosis induced by Zika virus infection in THP-1 cells

2020 ◽  
Vol 21 ◽  
Author(s):  
Morganna C. Lima ◽  
Elisa A. N. Azevedo ◽  
Clarice N. L. de Morais ◽  
Larissa I. O. de Sousa ◽  
Bruno M. Carvalho ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Virus Infection ◽  
Virus Replication ◽  
Zika Virus ◽  
Mammalian Cells ◽  
Caspase 3 ◽  
Neurological Complications ◽  
Zika Virus Infection

Background: Zika virus is an emerging arbovirus of global importance. ZIKV infection is associated with a range of neurological complications such as the Congenital Zika Syndrome and Guillain Barré Syndrome. Despite the magnitude of recent outbreaks, there is no specific therapy to prevent or to alleviate disease pathology. Objective: To investigate the role of P-MAPA immunomodulator in Zika-infected THP-1 cells. Methods: THP-1 cells were subjected at Zika virus infection (Multiplicity of Infection = 0.5) followed by treatment with P-MAPA for until 96 hours post-infection. After that, the cell death was analyzed by annexin+/ PI+ and caspase 3/ 7+ staining by flow cytometry. In addition, the virus replication and cell proliferation were accessed by RT-qPCR and Ki67 staining, respectively. Results: We demonstrate that P-MAPA in vitro treatment significantly reduces Zika virus-induced cell death and caspase-3/7 activation on THP-1 infected cells, albeit it has no role in virus replication and cell proliferation. Conclusions: Our study reveals that P-MAPA seems to be a satisfactory alternative to inhibits the effects of Zika virus infection in mammalian cells.

scholarly journals Fludarabine Inhibits Infection of Zika Virus, SFTS Phlebovirus, and Enterovirus A71

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 774
Author(s):  
Chengfeng Gao ◽  
Chunxia Wen ◽  
Zhifeng Li ◽  
Shuhan Lin ◽  
Shu Gao ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Zika Virus ◽  
Therapeutic Agent ◽  
Viral Infections ◽  
Rna Virus ◽  
Emerging Viruses ◽  
Enterovirus A71 ◽  
Ic50 Values ◽  
High Doses ◽  
Severe Fever

Viral infections are one of the leading causes in human mortality and disease. Broad-spectrum antiviral drugs are a powerful weapon against new and re-emerging viruses. However, viral resistance to existing broad-spectrum antivirals remains a challenge, which demands development of new broad-spectrum therapeutics. In this report, we showed that fludarabine, a fluorinated purine analogue, effectively inhibited infection of RNA viruses, including Zika virus, Severe fever with thrombocytopenia syndrome virus, and Enterovirus A71, with all IC50 values below 1 μM in Vero, BHK21, U251 MG, and HMC3 cells. We observed that fludarabine has shown cytotoxicity to these cells only at high doses indicating it could be safe for future clinical use if approved. In conclusion, this study suggests that fludarabine could be developed as a potential broad-spectrum anti-RNA virus therapeutic agent.

Valosin‐containing protein ATPase activity regulates the morphogenesis of Zika virus replication organelles and virus‐induced cell death

2021 ◽  
Author(s):  
Anaïs Anton ◽  
Clément Mazeaud ◽  
Wesley Freppel ◽  
Claudia Gilbert ◽  
Nicolas Tremblay ◽  
...  
Keyword(s):  
Cell Death ◽  
Atpase Activity ◽  
Virus Replication ◽  
Zika Virus

Antiviral activity of pinocembrin against Zika virus replication

2019 ◽  
Vol 167 ◽  
pp. 13-24 ◽  
Author(s):  
Jia Le Lee ◽  
Marcus Wing Choy Loe ◽  
Regina Ching Hua Lee ◽  
Justin Jang Hann Chu
Keyword(s):  
Antiviral Activity ◽  
Virus Replication ◽  
Zika Virus

scholarly journals Variable Inhibition of Zika Virus Replication by Different Wolbachia Strains in Mosquito Cell Cultures

2017 ◽  
Vol 91 (14) ◽  
Author(s):  
Michaela J. Schultz ◽  
Sharon Isern ◽  
Scott F. Michael ◽  
Ronald B. Corley ◽  
John H. Connor ◽  
...  
Keyword(s):  
Virus Replication ◽  
Zika Virus ◽  
Western Hemisphere ◽  
Mosquito Cell ◽  
Mosquito Vector ◽  
Viral Inhibition ◽  
Virus Growth ◽  
Content Type ◽  
Bacterial Endosymbiont ◽  
Mosquito Cells

ABSTRACT Mosquito-borne arboviruses are a major source of human disease. One strategy to reduce arbovirus disease is to reduce the mosquito's ability to transmit virus. Mosquito infection with the bacterial endosymbiont Wolbachia pipientis wMel is a novel strategy to reduce Aedes mosquito competency for flavivirus infection. However, experiments investigating cyclic environmental temperatures have shown a reduction in maternal transmission of wMel, potentially weakening the integration of this strain into a mosquito population relative to that of other Wolbachia strains. Consequently, it is important to investigate additional Wolbachia strains. All Zika virus (ZIKV) suppression studies are limited to the wMel Wolbachia strain. Here we show ZIKV inhibition by two different Wolbachia strains: wAlbB (isolated from Aedes albopictus mosquitoes) and wStri (isolated from the planthopper Laodelphax striatellus) in mosquito cells. Wolbachia strain wStri inhibited ZIKV most effectively. Single-cycle infection experiments showed that ZIKV RNA replication and nonstructural protein 5 translation were reduced below the limits of detection in wStri-containing cells, demonstrating early inhibition of virus replication. ZIKV replication was rescued when Wolbachia was inhibited with a bacteriostatic antibiotic. We observed a partial rescue of ZIKV growth when Wolbachia-infected cells were supplemented with cholesterol-lipid concentrate, suggesting competition for nutrients as one of the possible mechanisms of Wolbachia inhibition of ZIKV. Our data show that wAlbB and wStri infection causes inhibition of ZIKV, making them attractive candidates for further in vitro mechanistic and in vivo studies and future vector-centered approaches to limit ZIKV infection and spread. IMPORTANCE Zika virus (ZIKV) has swiftly spread throughout most of the Western Hemisphere. This is due in large part to its replication in and spread by a mosquito vector host. There is an urgent need for approaches that limit ZIKV replication in mosquitoes. One exciting approach for this is to use a bacterial endosymbiont called Wolbachia that can populate mosquito cells and inhibit ZIKV replication. Here we show that two different strains of Wolbachia, wAlbB and wStri, are effective at repressing ZIKV in mosquito cell lines. Repression of virus growth is through the inhibition of an early stage of infection and requires actively replicating Wolbachia. Our findings further the understanding of Wolbachia viral inhibition and provide novel tools that can be used in an effort to limit ZIKV replication in the mosquito vector, thereby interrupting the transmission and spread of the virus.

scholarly journals Inhibition of rubella virus replication by the broad-spectrum drug nitazoxanide in cell culture and in a patient with a primary immune deficiency

2017 ◽  
Vol 147 ◽  
pp. 58-66 ◽  
Author(s):  
Ludmila Perelygina ◽  
Timo Hautala ◽  
Mikko Seppänen ◽  
Adebola Adebayo ◽  
Kathleen E. Sullivan ◽  
...  
Keyword(s):  
Immune Deficiency ◽  
Cell Culture ◽  
Virus Replication ◽  
Broad Spectrum ◽  
Rubella Virus ◽  
Primary Immune Deficiency

scholarly journals Human Sertoli cells support high levels of Zika virus replication and persistence

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Anil Kumar ◽  
Juan Jovel ◽  
Joaquin Lopez-Orozco ◽  
Daniel Limonta ◽  
Adriana M. Airo ◽  
...  
Keyword(s):  
Virus Replication ◽  
Sertoli Cells ◽  
Zika Virus

scholarly journals Toll-like receptor agonist R848 blocks Zika virus replication by inducing the antiviral protein viperin

Virology ◽  
2018 ◽  
Vol 522 ◽  
pp. 199-208 ◽  
Author(s):  
Bénédicte Vanwalscappel ◽  
Takuya Tada ◽  
Nathaniel R. Landau
Keyword(s):  
Virus Replication ◽  
Zika Virus ◽  
Receptor Agonist ◽  
Toll Like Receptor ◽  
Antiviral Protein
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