Immunization with a Synthetic Human MUC1 Glycopeptide Vaccine against Tumor‐Associated MUC1 Breaks Tolerance in Human MUC1 Transgenic Mice

ChemMedChem ◽  
2017 ◽  
Vol 12 (17) ◽  
pp. 1424-1428 ◽  
Author(s):  
Natascha Stergiou ◽  
Markus Glaffig ◽  
Helmut Jonuleit ◽  
Edgar Schmitt ◽  
Horst Kunz
Keyword(s):  
Transgenic Mice ◽  
Human Muc1

Reversal of tolerance to human MUC1 antigen in MUC1 transgenic mice immunized with MUC1-C3d fusion protein

2007 ◽  
Vol 44 (1-3) ◽  
pp. 221
Author(s):  
Rieko Ohta ◽  
Masaki Imai ◽  
Chun He ◽  
Ted M. Ross ◽  
Stephen Tomlinson
Keyword(s):  
Transgenic Mice ◽  
Fusion Protein ◽  
Human Muc1

scholarly journals Reversal of tolerance to human MUC1 antigen in MUC1 transgenic mice immunized with fusions of dendritic and carcinoma cells

1998 ◽  
Vol 95 (11) ◽  
pp. 6279-6283 ◽  
Author(s):  
J. Gong ◽  
D. Chen ◽  
M. Kashiwaba ◽  
Y. Li ◽  
L. Chen ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Carcinoma Cells ◽  
Human Muc1

MUC1-specific immune responses in human MUC1 transgenic mice immunized with various human MUC1 vaccines

2000 ◽  
Vol 48 (10) ◽  
pp. 588-594 ◽  
Author(s):  
Bruce Acres ◽  
Vasso Apostolopoulos ◽  
Jean-Marc Balloul ◽  
Danny Wreschner ◽  
Pei-Xiang Xing ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Immune Responses ◽  
Human Muc1

scholarly journals Analysis Of Lung Injury Models By Using Human MUC1 Transgenic Mice

2012 ◽  
Author(s):  
Mizu Sakai ◽  
Tetsuya Kubota ◽  
Takashi Yamane ◽  
Naoki Shiota ◽  
Hiroshi Ohnishi ◽  
...  
Keyword(s):  
Lung Injury ◽  
Transgenic Mice ◽  
Human Muc1 ◽  
Injury Models

Lung Injury Model Using Human MUC1 Transgenic Mice

2011 ◽  
Author(s):  
Mizu Sakai ◽  
Tetsuya Kubota ◽  
Mayuka Isaka ◽  
Takashi Yamane ◽  
Naoki Shiota ◽  
...  
Keyword(s):  
Lung Injury ◽  
Transgenic Mice ◽  
Injury Model ◽  
Human Muc1 ◽  
Lung Injury Model

Quantitative Microscopic Comparison of the Organization of the Lung in Transgenic Mice Expressing Transforming Growth Factor-α (TGF-α)

1996 ◽  
Vol 54 ◽  
pp. 14-15
Author(s):  
C. G. Plopper ◽  
C. Helton ◽  
A. J. Weir ◽  
J. A. Whitsett ◽  
T. R. Korfhagen
Keyword(s):  
Growth Factor ◽  
Pulmonary Fibrosis ◽  
Transgenic Mice ◽  
Blood Vessels ◽  
Lung Parenchyma ◽  
Lung Maturation ◽  
Alveolar Air ◽  
Parenchymal Tissue ◽  
Air Space ◽  
Conducting Airways

A wide variety of growth factors are thought to be involved in the regulation of pre- and postnatal lung maturation, including factors which bind to the epidermal growth factor receptor. Marked pulmonary fibrosis and enlarged alveolar air spaces have been observed in lungs of transgenic mice expressing human TGF-α under control of the 3.7 KB human SP-C promoter. To test whether TGF-α alters lung morphogenesis and cellular differentiation, we examined morphometrically the lungs of adult (6-10 months) mice derived from line 28, which expresses the highest level of human TGF-α transcripts among transgenic lines. Total volume of lungs (LV) fixed by airway infusion at standard pressure was similar in transgenics and aged-matched non-transgenic mice (Fig. 1). Intrapulmonary bronchi and bronchioles made up a smaller percentage of LV in transgenics than in non-transgenics (Fig. 2). Pulmonary arteries and pulmonary veins were a smaller percentage of LV in transgenic mice than in non-transgenics (Fig. 3). Lung parenchyma (lung tissue free of large vessels and conducting airways) occupied a larger percentage of LV in transgenics than in non-transgenics (Fig. 4). The number of generations of branching in conducting airways was significantly reduced in transgenics as compared to non-transgenic mice. Alveolar air space size, as measured by mean linear intercept, was almost twice as large in transgenic mice as in non-transgenics, especially when different zones within the lung were compared (Fig. 5). Alveolar air space occupied a larger percentage of the lung parenchyma in transgenic mice than in non-transgenic mice (Fig. 6). Collagen abundance was estimated in histological sections as picro-Sirius red positive material by previously-published methods. In intrapulmonary conducting airways, collagen was 4.8% of the wall in transgenics and 4.5% of the wall in non-transgenic mice. Since airways represented a smaller percentage of the lung in transgenics, the volume of interstitial collagen associated with airway wall was significantly less. In intrapulmonary blood vessels, collagen was 8.9% of the wall in transgenics and 0.7% of the wall in non-transgenics. Since blood vessels were a smaller percentage of the lungs in transgenics, the volume of collagen associated with the walls of blood vessels was five times greater. In the lung parenchyma, collagen was 51.5% of the tissue volume in transgenics and 21.2% in non-transgenics. Since parenchyma was a larger percentage of lung volume in transgenics, but the parenchymal tissue was a smaller percent of the volume, the volume of collagen associated with parenchymal tissue was only slightly greater. We conclude that overexpression of TGF-α during lung maturation alters many aspects of lung development, including branching morphogenesis of the airways and vessels and alveolarization in the parenchyma. Further, the increases in visible collagen previously associated with pulmonary fibrosis due to the overexpression of TGF-α are a result of actual increases in amounts of collagen and in a redistribution of collagen within compartments which results from morphogenetic changes. These morphogenetic changes vary by lung compartment. Supported by HL20748, ES06700 and the Cystic Fibrosis Foundation.

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