Ultrastructure and immunocytochemistry of the isolated human erythrocyte membrane skeleton

1993 ◽  
Vol 25 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Jeanine A. Ursitti ◽  
James B. Wade
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Membrane Skeleton ◽  
Human Erythrocyte Membrane

Glycophorin is linked by band 4.1 protein to the human erythrocyte membrane skeleton

Nature ◽  
1984 ◽  
Vol 307 (5952) ◽  
pp. 655-658 ◽  
Author(s):  
Richard A. Anderson ◽  
Rex E. Lovrien
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Membrane Skeleton ◽  
Human Erythrocyte Membrane

The human erythrocyte membrane skeleton may be an ionic gel

1986 ◽  
Vol 13 (4) ◽  
pp. 219-233 ◽  
Author(s):  
B. T. Stokke ◽  
A. Mikkelsen ◽  
A. Elgsaeter
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Membrane Skeleton ◽  
Human Erythrocyte Membrane

scholarly journals Analysis of Integral Membrane Protein Contributions to the Deformability and Stability of the Human Erythrocyte Membrane

2001 ◽  
Vol 276 (50) ◽  
pp. 46968-46974 ◽  
Author(s):  
Heidi M. Van Dort ◽  
David W. Knowles ◽  
Joel A. Chasis ◽  
Gloria Lee ◽  
Narla Mohandas ◽  
...  
Keyword(s):  
Surface Area ◽  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Membrane Skeleton ◽  
Band 3 ◽  
Membrane Stability ◽  
Human Erythrocyte Membrane ◽  
Lipid Interactions ◽  
Membrane Spanning ◽  
The Impact

Three major hypotheses have been proposed to explain the role of membrane-spanning proteins in establishing/maintaining membrane stability. These hypotheses ascribe the essential contribution of integral membrane proteins to (i) their ability to anchor the membrane skeleton to the lipid bilayer, (ii) their capacity to bind and stabilize membrane lipids, and (iii) their ability to influence and regulate local membrane curvature. In an effort to test these hypotheses in greater detail, we have modified both the membrane skeletal and lipid binding interactions of band 3 (the major membrane-spanning and skeletal binding protein of the human erythrocyte membrane) and have examined the impact of these modifications on erythrocyte membrane morphology, deformability, and stability. The desired changes in membrane skeletal and protein-lipid interactions were induced by 1) reaction of the cells with 4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS), an inhibitor of band 3-mediated anion transport that dissociates band 3 into dimers (increasing its surface area in contact with lipid) and severs band 3 linkages to the membrane skeleton; 2) a fragment of ankyrin that ruptures the same ankyrin-band 3 bridge to the membrane skeleton, but drives the band 3 subunit equilibrium toward the tetramer (i.e.decreasing the band 3 surface area in contact with lipid); and 3) an antibody to the ankyrin-binding site on band 3 that promotes the same changes in band 3 skeletal and lipid interactions as the ankyrin fragment. We observed that although DIDS induced echinocytic morphological changes in the treated erythrocytes, it had little impact on either membrane deformability or stability. In contrast, resealing of either the ankyrin fragment or anti-band 3 IgG into erythrocytes caused spontaneous membrane fragmentation and loss of deformability/stability. Because these and other new observations cannot all be reconciled with any single hypothesis on membrane stability, we suggest that more than one hypothesis may be operative and provide an explanation of how each might individually contribute to net membrane stability.

The human erythrocyte membrane skeleton may be an ionic gel

1986 ◽  
Vol 13 (4) ◽  
pp. 203-218 ◽  
Author(s):  
B. T. Stokke ◽  
A. Mikkelsen ◽  
A. Elgsaeter
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Membrane Skeleton ◽  
Human Erythrocyte Membrane
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