Cytoskeletal dynamics in rabbit synovial fibroblasts: I. Effects of acrylamide on intermediate filaments and microfilaments

1990 ◽  
Vol 16 (2) ◽  
pp. 110-120 ◽  
Author(s):  
Judith Aggeler ◽  
Keith Seely
Keyword(s):  
Intermediate Filaments ◽  
Synovial Fibroblasts ◽  
Cytoskeletal Dynamics

scholarly journals Vimentin intermediate filaments stabilize dynamic microtubules by direct interactions

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Laura Schaedel ◽  
Charlotta Lorenz ◽  
Anna V. Schepers ◽  
Stefan Klumpp ◽  
Sarah Köster
Keyword(s):  
Intermediate Filaments ◽  
Cell Mechanics ◽  
Physical Nature ◽  
Cellular Functions ◽  
Interaction Forces ◽  
In Vitro System ◽  
Direct Mechanism ◽  
Cytoskeletal Dynamics ◽  
Insight Into

AbstractThe cytoskeleton determines cell mechanics and lies at the heart of important cellular functions. Growing evidence suggests that the manifold tasks of the cytoskeleton rely on the interactions between its filamentous components—actin filaments, intermediate filaments, and microtubules. However, the nature of these interactions and their impact on cytoskeletal dynamics are largely unknown. Here, we show in a reconstituted in vitro system that vimentin intermediate filaments stabilize microtubules against depolymerization and support microtubule rescue. To understand these stabilizing effects, we directly measure the interaction forces between individual microtubules and vimentin filaments. Combined with numerical simulations, our observations provide detailed insight into the physical nature of the interactions and how they affect microtubule dynamics. Thus, we describe an additional, direct mechanism by which cells establish the fundamental cross talk of cytoskeletal components alongside linker proteins. Moreover, we suggest a strategy to estimate the binding energy of tubulin dimers within the microtubule lattice.

scholarly journals Vimentin Intermediate Filaments Stabilize Dynamic Microtubules by Direct Interactions

2020 ◽  
Author(s):  
Laura Schaedel ◽  
Charlotta Lorenz ◽  
Anna V. Schepers ◽  
Stefan Klumpp ◽  
Sarah Köster
Keyword(s):  
Intermediate Filaments ◽  
Cell Mechanics ◽  
Physical Nature ◽  
Cellular Functions ◽  
In Vitro System ◽  
Direct Mechanism ◽  
Cytoskeletal Dynamics ◽  
Novel Strategy ◽  
Insight Into

AbstractThe cytoskeleton determines cell mechanics and lies at the heart of important cellular functions. Growing evidence suggests that the manifold tasks of the cytoskeleton rely on the interactions between its filamentous components, known as actin filaments, intermediate filaments and microtubules. However, the nature of these interactions and their impact on cytoskeletal dynamics are largely unknown. Here, we show in a re-constituted in vitro system that vimentin intermediate filaments stabilize microtubules against depolymerization and support microtubule rescue. To understand these stabilizing effects, we directly measure the interaction forces between individual microtubules and vimentin filaments. Combined with numerical simulations, our observations provide detailed insight into the physical nature of the interactions and how they affect microtubule dynamics. Thus, we describe an additional, direct mechanism for cells to establish the fundamental cross-talk of cytoskeletal components alongside linker proteins. Moreover, we suggest a novel strategy to estimate the binding energy of tubulin dimers within the microtubule lattice.

Filaments and membranes in the mitotic apparatus

1983 ◽  
Vol 41 ◽  
pp. 544-547
Author(s):  
Kent McDonald
Keyword(s):  
Intermediate Filaments ◽  
Mitotic Spindle ◽  
Mitotic Apparatus ◽  
Light Microscope Level ◽  
Microtubule Associated Proteins ◽  
Recent Developments ◽  
Associated Proteins ◽  
Force Generator ◽  
Spindle Function ◽  
Antibody Technology

At the light microscope level the recent developments and interest in antibody technology have permitted the localization of certain non-microtubule proteins within the mitotic spindle, e.g., calmodulin, actin, intermediate filaments, protein kinases and various microtubule associated proteins. Also, the use of fluorescent probes like chlorotetracycline suggest the presence of membranes in the spindle. Localization of non-microtubule structures in the spindle at the EM level has been less rewarding. Some mitosis researchers, e.g., Rarer, have maintained that actin is involved in mitosis movements though the bulk of evidence argues against this interpretation. Others suggest that a microtrabecular network such as found in chromatophore granule movement might be a possible force generator but there is little evidence for or against this view. At the level of regulation of spindle function, Harris and more recently Hepler have argued for the importance of studying spindle membranes. Hepler also believes that membranes might play a structural or mechanical role in moving chromosomes.

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