Correlated distribution of actin, myosin, and microtubules at the leading edge of migrating swiss 3T3 fibroblasts

1989 ◽  
Vol 14 (4) ◽  
pp. 527-543 ◽  
Author(s):  
Patricia A. Conrad ◽  
Michel A. Nederlof ◽  
Ira M. Herman ◽  
D. Lansing Taylor
Keyword(s):  
Leading Edge ◽  
3T3 Fibroblasts ◽  
Swiss 3T3 ◽  
Swiss 3T3 Fibroblasts

scholarly journals Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Michiko Sugawara ◽  
Hiromi Miyoshi ◽  
Takuya Miura ◽  
Hiroto Tanaka ◽  
Ken-ichi Tsubota ◽  
...  
Keyword(s):  
Focal Adhesions ◽  
Leading Edge ◽  
Stress Fibers ◽  
Lateral Region ◽  
3T3 Fibroblasts ◽  
Self Assembling ◽  
Swiss 3T3 ◽  
Cell Retraction ◽  
Swiss 3T3 Fibroblasts ◽  
Actin Stress Fibers

To understand the mechanism regulating the spontaneous change in polarity that leads to cell turning, we quantitatively analyzed the dynamics of focal adhesions (FAs) coupling with the self-assembling actin cytoskeletal structure in Swiss 3T3 fibroblasts. Fluorescent images were acquired from cells expressing GFP-actin and RFP-zyxin by laser confocal microscopy. On the basis of the maximum area, duration, and relocation distance of FAs extracted from the RFP-zyxin images, the cells could be divided into 3 regions: the front region, intermediate lateral region, and rear region. In the intermediate lateral region, FAs appeared close to the leading edge and were stabilized gradually as its area increased. Simultaneously, bundled actin stress fibers (SFs) were observed vertically from the positions of these FAs, and they connected to the other SFs parallel to the leading edge. Finally, these connecting SFs fused to form a single SF with matured FAs at both ends. This change in SF organization with cell retraction in the first cycle of migration followed by a newly formed protrusion in the next cycle is assumed to lead to cell turning in migrating Swiss 3T3 fibroblasts.

scholarly journals ROCK and mDia1 antagonize in Rho-dependent Rac activation in Swiss 3T3 fibroblasts

2002 ◽  
Vol 157 (5) ◽  
pp. 819-830 ◽  
Author(s):  
Takahiro Tsuji ◽  
Toshimasa Ishizaki ◽  
Muneo Okamoto ◽  
Chiharu Higashida ◽  
Kazuhiro Kimura ◽  
...  
Keyword(s):  
Focal Adhesions ◽  
Small Gtpase ◽  
Dominant Negative ◽  
Stress Fibers ◽  
Induced Membrane ◽  
3T3 Fibroblasts ◽  
C3 Exoenzyme ◽  
Swiss 3T3 ◽  
Swiss 3T3 Fibroblasts ◽  
Membrane Ruffle

The small GTPase Rho acts on two effectors, ROCK and mDia1, and induces stress fibers and focal adhesions. However, how ROCK and mDia1 individually regulate signals and dynamics of these structures remains unknown. We stimulated serum-starved Swiss 3T3 fibroblasts with LPA and compared the effects of C3 exoenzyme, a Rho inhibitor, with those of Y-27632, a ROCK inhibitor. Y-27632 treatment suppressed LPA-induced formation of stress fibers and focal adhesions as did C3 exoenzyme but induced membrane ruffles and focal complexes, which were absent in the C3 exoenzyme-treated cells. This phenotype was suppressed by expression of N17Rac. Consistently, the amount of GTP-Rac increased significantly by Y-27632 in LPA-stimulated cells. Biochemically, Y-27632 suppressed tyrosine phosphorylation of paxillin and focal adhesion kinase and not that of Cas. Inhibition of Cas phosphorylation with PP1 or expression of a dominant negative Cas mutant inhibited Y-27632–induced membrane ruffle formation. Moreover, Crk-II mutants lacking in binding to either phosphorylated Cas or DOCK180 suppressed the Y-27632–induced membrane ruffle formation. Finally, expression of a dominant negative mDia1 mutant also inhibited the membrane ruffle formation by Y-27632. Thus, these results have revealed the Rho-dependent Rac activation signaling that is mediated by mDia1 through Cas phosphorylation and antagonized by the action of ROCK.

cDNA-cloning, sequencing and expression in glucocorticoid-stimulated quiescent Swiss 3T3 fibroblasts of mouse lipocortin I

1989 ◽  
Vol 159 (1) ◽  
pp. 155-162 ◽  
Author(s):  
Christine Philipps ◽  
Stefan Rose-John ◽  
Gabriele Rincke ◽  
Gerhard Fürstenberger ◽  
Friedrich Marks
Keyword(s):  
Cdna Cloning ◽  
3T3 Fibroblasts ◽  
Swiss 3T3 ◽  
Sequencing And Expression ◽  
Swiss 3T3 Fibroblasts

The flightless I protein colocalizes with actin- and microtubule-based structures in motile Swiss 3T3 fibroblasts: evidence for the involvement of PI 3-kinase and Ras-related small GTPases

2001 ◽  
Vol 114 (3) ◽  
pp. 549-562 ◽  
Author(s):  
D.A. Davy ◽  
H.D. Campbell ◽  
S. Fountain ◽  
D. de Jong ◽  
M.F. Crouch
Keyword(s):  
Specific Inhibitor ◽  
Small Gtpases ◽  
Small Gtpase ◽  
Actin Binding ◽  
Serum Stimulation ◽  
3T3 Fibroblasts ◽  
Swiss 3T3 ◽  
Green Fluorescent ◽  
Cytoskeletal Rearrangements ◽  
Swiss 3T3 Fibroblasts

The flightless I protein contains an actin-binding domain with homology to the gelsolin family and is likely to be involved in actin cytoskeletal rearrangements. It has been suggested that this protein is involved in linking the cytoskeletal network with signal transduction pathways. We have developed antibodies directed toward the leucine rich repeat and gelsolin-like domains of the human and mouse homologues of flightless I that specifically recognize expressed and endogenous forms of the protein. We have also constructed a flightless I-enhanced green fluorescent fusion vector and used this to examine the localization of the expressed protein in Swiss 3T3 fibroblasts. The flightless I protein localizes predominantly to the nucleus and translocates to the cytoplasm following serum stimulation. In cells stimulated to migrate, the flightless I protein colocalizes with beta-tubulin- and actin-based structures. Members of the small GTPase family, also implicated in cytoskeletal control, were found to colocalize with flightless I in migrating Swiss 3T3 fibroblasts. LY294002, a specific inhibitor of PI 3-kinase, inhibits the translocation of flightless I to actin-based structures. Our results suggest that PI 3-kinase and the small GTPases, Ras, RhoA and Cdc42 may be part of a common functional pathway involved in Fliih-mediated cytoskeletal regulation. Functionally, we suggest that flightless I may act to prepare actin filaments or provide factors required for cytoskeletal rearrangements necessary for cell migration and/or adhesion.

A voltage-dependent calcium current in mouse Swiss 3T3 fibroblasts

1988 ◽  
Vol 411 (5) ◽  
pp. 554-557 ◽  
Author(s):  
A. Peres ◽  
R. Zippel ◽  
E. Sturani ◽  
G. Mostacciuolo
Keyword(s):  
Calcium Current ◽  
3T3 Fibroblasts ◽  
Voltage Dependent ◽  
Swiss 3T3 ◽  
Swiss 3T3 Fibroblasts
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