Fluorescence microscopic localization of actin in pollen tubes: Comparison of actin antibody and phalloidin staining

1989 ◽  
Vol 12 (4) ◽  
pp. 216-224 ◽  
Author(s):  
Xiaojing Tang ◽  
Susan A. Lancelle ◽  
Peter K. Hepler
Keyword(s):  
Pollen Tubes ◽  
Phalloidin Staining ◽  
Microscopic Localization

The electron microscopic localization of nephrotoxic antibodies in isolated glomeruli

Pathology ◽  
1976 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
I.P. McCausland ◽  
R.N. Seelye ◽  
J.B. Gavin ◽  
P.B. Herdson
Keyword(s):  
Electron Microscopic ◽  
Isolated Glomeruli ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

scholarly journals Villin controls the formation and enlargement of punctate actin foci in pollen tubes

2020 ◽  
Vol 133 (6) ◽  
pp. jcs237404 ◽  
Author(s):  
Wanying Zhao ◽  
Xiaolu Qu ◽  
Yuhui Zhuang ◽  
Ludi Wang ◽  
Maurice Bosch ◽  
...  
Keyword(s):  
Pollen Tubes

Keeping pollen tubes on track

Nature Plants ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 51-52 ◽  
Author(s):  
Yan Ju ◽  
Sharon A. Kessler
Keyword(s):  
Pollen Tubes

scholarly journals Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth

2021 ◽  
Vol 40 (2) ◽  
pp. 205-222
Author(s):  
Monica Scali ◽  
Alessandra Moscatelli ◽  
Luca Bini ◽  
Elisabetta Onelli ◽  
Rita Vignani ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Membrane Trafficking ◽  
Tip Growth ◽  
Male Gametophyte ◽  
Pollen Tubes ◽  
Structural Features ◽  
Two Dimensional Gel Electrophoresis ◽  
Depth Analysis

AbstractPollen tube elongation is characterized by a highly-polarized tip growth process dependent on an efficient vesicular transport system and largely mobilized by actin cytoskeleton. Pollen tubes are an ideal model system to study exocytosis, endocytosis, membrane recycling, and signaling network coordinating cellular processes, structural organization and vesicular trafficking activities required for tip growth. Proteomic analysis was applied to identifyNicotiana tabacumDifferentially Abundant Proteins (DAPs) after in vitro pollen tube treatment with membrane trafficking inhibitors Brefeldin A, Ikarugamycin and Wortmannin. Among roughly 360 proteins separated in two-dimensional gel electrophoresis, a total of 40 spots visibly changing between treated and control samples were identified by MALDI-TOF MS and LC–ESI–MS/MS analysis. The identified proteins were classified according to biological processes, and most proteins were related to pollen tube energy metabolism, including ammino acid synthesis and lipid metabolism, structural features of pollen tube growth as well modification and actin cytoskeleton organization, stress response, and protein degradation. In-depth analysis of proteins corresponding to energy-related pathways revealed the male gametophyte to be a reliable model of energy reservoir and dynamics.

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