High hydrostatic pressure effects in vivo: Changes in cell morphology, microtubule assembly, and actin organization

1988 ◽  
Vol 10 (3) ◽  
pp. 380-390 ◽  
Author(s):  
Brenda Bourns ◽  
Samuel Franklin ◽  
Lynne Cassimeris ◽  
E. D. Salmon
Keyword(s):  
Hydrostatic Pressure ◽  
Cell Morphology ◽  
High Hydrostatic Pressure ◽  
Pressure Effects ◽  
Microtubule Assembly ◽  
Actin Organization

High hydrostatic pressure effects on Listeria monocytogenes and L. innocua : Evidence for variability in inactivation behaviour and in resistance to pediocin bacHA-6111-2

2017 ◽  
Vol 64 ◽  
pp. 226-231 ◽  
Author(s):  
Carolina Bruschi ◽  
Norton Komora ◽  
Sónia Marília Castro ◽  
Jorge Saraiva ◽  
Vânia Borges Ferreira ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Pressure Effects

scholarly journals Preparation of a Nanoscaled Poly(vinyl alcohol)/Hydroxyapatite/DNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Tsuyoshi Kimura ◽  
Yoichi Nibe ◽  
Seiichi Funamoto ◽  
Masahiro Okada ◽  
Tsutomu Furuzono ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Vinyl Alcohol ◽  
Transgene Expression ◽  
Transfection Efficiency ◽  
Endosomal Escape ◽  
Poly Vinyl Alcohol ◽  
Dna Nanoparticles ◽  
Novel Method

Our previous research showed that poly(vinyl alcohol) (PVA) nanoparticles incorporating DNA with hydrogen bonds obtained by high hydrostatic pressurization are able to deliver DNA without any significant cytotoxicity. To enhance transfection efficiency of PVA/DNA nanoparticles, we describe a novel method to prepare PVA/DNA nanoparticles encapsulating nanoscaled hydroxyapatites (HAps) prepared by high hydrostatic pressurization (980 MPa), which is designed to facilitate endosomal escape induced by dissolving HAps in an endosome. Scanning electron microscopic observation and dynamic light scattering measurement revealed that HAps were significantly encapsulated in PVA/HAp/DNA nanoparticles. The cytotoxicity, cellular uptake, and transgene expression of PVA/HAp/DNA nanoparticles were investigated using COS-7 cells. It was found that, in contrast to PVA/DNA nanoparticles, their internalization and transgene expression increased without cytotoxicity occurring. Furthermore, a similar level of transgene expression between plasmid DNA and PVA/HAp/DNA nanoparticles was achieved using in vivo hydrodynamic injection. Our results show a novel method of preparing PVA/DNA nanoparticles encapsulating HAp nano-crystals by using high hydrostatic pressure technology and the potential use of HAps as an enhancer of the transfection efficiency of PVA/DNA nanoparticles without significant cytotoxicity.

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