Localization of sea urchin egg cytoplasmic dynein in mitotic apparatus studied by using a monoclonal antibody against sea urchin sperm flagellar 21S dynein

1987 ◽  
Vol 7 (2) ◽  
pp. 97-109 ◽  
Author(s):  
Shin-Ichi Hisanaga ◽  
Takeshi Tanaka ◽  
Tomoh Masaki ◽  
Hikoichi Sakai ◽  
Issei Mabuchi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sea Urchin ◽  
Cytoplasmic Dynein ◽  
Mitotic Apparatus ◽  
Sea Urchin Egg ◽  
Sea Urchin Sperm

scholarly journals A Quantitative Description of Protoplasmic Movement During Cleavage in the Sea-Urchin Egg

1958 ◽  
Vol 35 (2) ◽  
pp. 407-424
Author(s):  
Y. HIRAMOTO
Keyword(s):  
Cell Division ◽  
Sea Urchin ◽  
Quantitative Description ◽  
Motive Force ◽  
Mitotic Apparatus ◽  
Band Theory ◽  
Cytoplasmic Granules ◽  
Carbon Particles ◽  
Sea Urchin Egg ◽  
Protoplasmic Movement

1. Protoplasmic movements during cleavage in the eggs of the heart-urchin Clypeaster japonicus have been followed by tracing the movements of cytoplasmic granules and of carbon particles adhering to the surface. 2. These movements are quantitatively described in normal eggs and in eggs whose mitotic apparatus has been destroyed by colchicine. 3. The results obtained are qualitatively similar to those obtained by Spek and by Dan and his collaborators. 4. Endoplasmic movement and changes in the length and shape of the astral rays are readily explained by the contracting-ring (band) theory. 5. The location of the motive force of cell division is discussed.

Cyclic ADP-ribose signaling in sea urchin gametes: metabolism in spermatozoa

1997 ◽  
Vol 272 (2) ◽  
pp. C416-C420 ◽  
Author(s):  
E. N. Chini ◽  
M. A. Thompson ◽  
C. C. Chini ◽  
T. P. Dousa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sea Urchin ◽  
High Performance ◽  
Hplc Analysis ◽  
Sea Urchin Egg ◽  
Cyclic Adp Ribose ◽  
Ambient Content ◽  
Homologous Desensitization ◽  
Sea Urchin Sperm

The molecular mechanism that initiates Ca2+ signaling in sea urchin egg fertilization has not yet been clarified. To determine whether sea urchin sperm may generate and possibly supply cyclic ADP-ribose (cADPR) as a Ca2+-releasing factor in the course of sea urchin egg fertilization, we determined cADPR content and the capacity for cADPR synthesis in sea urchin sperm. cADPR content was determined using the sea urchin egg homogenate Ca2+-release bioassay combined with high-performance liquid chromatography (HPLC). We found that sperm homogenates synthesized cADPR from beta-NAD but did not synthesize cADPR when alpha-NAD was the substrate. The identity of cADPR generated by sperm homogenates was verified by HPLC analysis, use of specific Ca2+-release antagonists, and homologous desensitization of the sea urchin egg homogenate Ca2+-release bioassay. The ambient content of cADPR was approximately 0.3 nmol cADPR/g wet wt sea urchin sperm. Our results show that sperm can synthesize cADPR and that they contain cADPR levels comparable to other tissues.

A Ca-activated ATPase in the mitotic apparatus of the sea urchin egg (isolated by a new method)

1972 ◽  
Vol 70 (2) ◽  
pp. 325-332 ◽  
Author(s):  
D. Mazia ◽  
Chr. Petzelt ◽  
R.O. Williams ◽  
I. Meza
Keyword(s):  
Sea Urchin ◽  
New Method ◽  
Mitotic Apparatus ◽  
Sea Urchin Egg

scholarly journals SELECTIVE EXTRACTION OF ISOLATED MITOTIC APPARATUS

1971 ◽  
Vol 48 (2) ◽  
pp. 324-339 ◽  
Author(s):  
Thomas Bibring ◽  
Jane Baxandall
Keyword(s):  
Sea Urchin ◽  
Protein Extraction ◽  
Sedimentation Coefficient ◽  
Selective Extraction ◽  
Major Protein ◽  
Mitotic Apparatus ◽  
Sperm Tail ◽  
Microtubule Protein ◽  
Related Proteins ◽  
Sea Urchin Sperm

Mitotic apparatus isolated from sea urchin eggs has been treated with meralluride sodium under conditions otherwise resembling those of its isolation. The treatment causes a selective morphological disappearance of microtubules while extracting a major protein fraction, probably consisting of two closely related proteins, which constitutes about 10% of mitotic apparatus protein. Extraction of other cell particulates under similar conditions yields much less of this protein. The extracted protein closely resembles outer doublet microtubule protein from sea urchin sperm tail in properties considered typical of microtubule proteins: precipitation by calcium ion and vinblastine, electrophoretic mobility in both acid and basic polyacrylamide gels, sedimentation coefficient, molecular weight, and, according to a preliminary determination, amino acid composition. An antiserum against a preparation of sperm tail outer doublet microtubules cross-reacts with the extract from mitotic apparatus. On the basis of these findings it appears that microtubule protein is selectively extracted from isolated mitotic apparatus by treatment with meralluride, and is a typical microtubule protein.

scholarly journals Quantitative studies on the polarization optical properties of living cells II. The role of microtubules in birefringence of the spindle of the sea urchin egg.

1981 ◽  
Vol 89 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Y Hiramoto ◽  
Y Hamaguchi ◽  
Y Shóji ◽  
T E Schroeder ◽  
S Shimoda ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Sea Urchin ◽  
Preceding Paper ◽  
Living Cells ◽  
Mitotic Apparatus ◽  
Form Birefringence ◽  
Quantitative Studies ◽  
Sea Urchin Egg ◽  
Interpolar Distance

Birefringence of the mitotic apparatus (MA) and its change during mitosis in sea urchin eggs were quantitatively determined using the birefringence detection apparatus reported in the preceding paper (Hiramoto el al., 1981, J. Cell Biol. 89:115-120). The birefringence and the form of the MA are represented by five parameters: peak retardation (delta p), through retardation (delta t), interpolar distance (D1), the distance (D2) between chromosome groups moving toward poles, and the distance (D3) between two retardation peaks. Distributions of birefringence retardation and the coefficient of birefringence in the spindle were quantitatively determined in MAs isolated during metaphase and anaphase. The distribution of microtubules (MTs) contained in the spindle is attributable to the form birefringence caused by regularly arranged MTs. The distribution coincided fairly well with the distribution of MTs in isolated MAs determined by electron microscopy. Under the same assumption, the distribution of MTS in the spindle in living cells during mitosis was determined. The results show that the distribution of MTs and the total amount of polymerized tubulin (MTs) in the spindle change during mitosis, suggesting the assembly and disassembly of MTs as well as the dislocation of MTs during mitosis.

Substructure of sea urchin egg cytoplasmic dynein

1987 ◽  
Vol 195 (4) ◽  
pp. 919-927 ◽  
Author(s):  
Shin-ichi Hisanaga ◽  
Nobutaka Hirokawa
Keyword(s):  
Sea Urchin ◽  
Cytoplasmic Dynein ◽  
Sea Urchin Egg
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